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Study on the effect of sucrose concentration on potato cells original study
Study on the effect of sucrose concentration on potato cells original study
Effect of sucrose concentration on a potato
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Investigating the Effect of Different Concentrations of Sucrose Solutions on Potato Tiessue
Planning
For my GCSE biology coursework, I am going to conduct an investigation
into the effect of different concentrations of sucrose solutions on
potato tissue.
Text Box: A measuring cylinder
Apparatus
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18 test tubes
[IMAGE][IMAGE]
Text Box: 1M sucrose solution
[IMAGE]A test tube rack
[IMAGE]
Cork borer
Text Box: Distilled water[IMAGE]
[IMAGE]
A potato
Text Box: White tile
[IMAGE]
[IMAGE]
Balance
Text Box: Scalpel[IMAGE]
[IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]
Prediction
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As the concentration of the sucrose solution increases, the mass of
the potato cylinders will decrease. This is because osmosis is taking
place. Osmosis is the diffusion of water from a higher concentration
of water to a lower concentration of water through a selectively
permeable membrane. When the potato in is put into distilled water, it
will get bigger as water will enter its cells by osmosis. This is
because the water is more concentrated outside the potato rather than
inside the potato. The water will stop when the potato cells are
turgid. When the potato is put into the 1M sucrose solution, it will
get smaller as water will leave its cells by osmosis. This is because
the water in more concentrated inside the potato rather than outside
the potato. Cells with a little water in them are known as flaccid.
When a cell becomes too flaccid, it could be destroyed. A process
called plasmolysis would occur – this is where the cell membrane comes
away from the cell wall so there are big empty spaces between them.
The graph will have a negative correlation because as the
concentration of the sucrose solution increases; the mass of the
potato cylinders will decrease.
Preliminary work
================
Concentration of
sucrose solution (M)
Length of potato (cm)
Before
After
Difference
0.0
5.
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
Investigating Osmosis in Potatoes Aim: Investigate the movement of osmosis through a selectively permeable membrane, in this case potato. Introduction: Osmosis is the movement of water through a semi permeable membrane. The water passes from an area of high concentration to an area of low concentration, until the two concentrations are equal in concentrations of water. Many cell membranes behave as semi permeable membranes, and osmosis is a vital part in the movement of liquids in living organisms, for example, in the transport of water from the soil to the roots in plants.
Purpose: This lab gives the idea about the enzyme. We will do two different experiments. Enzyme is a protein that made of strings of amino acids and it is helping to produce chemical reactions in the quickest way. In the first experiment, we are testing water, sucrose solution, salt solution, and hydrogen peroxide to see which can increase the bubbles. So we can understand that enzyme producing chemical reactions in the speed. In the second experiment, we are using temperature of room, boiling water, refrigerator, and freezer to see what will effect the enzyme.
We then cut our potato tubes with the cork borer and cut them with the scalpel so they were the same length and weighed them. We then put one potato tube in each test tube and then added the same amount sugar solution in to each tube. The concentration of sugar solutions varied in each test tube.
How the Concentration of the Substrate Affects the Reaction in the Catalase Inside Potato Cells
The purpose of this experiment is to investigate the effect of changing the concentration of sodium chloride solution on the rate of osmosis in tubes of potatoes. This was maintained using equal measurements of the potato tubes and applying them into the different concentrations of sodium chloride, 0%, 2%, 5%, 10%, 20% and 26%, in beakers then measuring the change in mass of the potato tubes afterwards. The time taken for all potato tubes to be placed in solution was 15 minutes. Can the concentration gradient of the sodium chloride solution influence on the rate of osmotic diffusion undergone by the potato tubes?
Influence of Temperature on the Activity of Potato Catalase Hypothesis That the higher the temperature the higher the reaction rate of potato catalyse to a point were denaturing occurs in the enzyme and the reaction rate of the potato catalase drops off. Prediction The rate of Catalase activity will be faster at higher temperatures until a point, because at higher temperatures there are more chances of collisions between the enzyme's (Catalase) active site and the substrate (hydrogen peroxide). However the rate depends on the active site being able to join with the substrate, and at higher temperatures the enzyme can be denatured, which changes the shape of the active site which thus prevents the reaction from happening. At first, as the temperature increases the activity of the Potato catalase also increases this is because the collision rate of the enzyme with the hydrogen peroxide is increased.
To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
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Equipment Potato, Borer, Beakers, Measuring Cylinder, Stopclocks, Distilled Water, Electronic Balance, Salt solutions of various concentrations. Diagram [IMAGE] [IMAGE] [IMAGE] When we leave the potato in the solution for the allocated time, water
...e the graph falls more steeply, flattening off at higher concentrations. I would also like to conduct an investigation, in conjunction with this experiment, to calculate the concentration of sap within the vacuole.
there would be no flow of water into or out of the cell so the cell
For my preliminary work, I used a 50 mm piece of potato. It was easy
potato cells. In order to find the best way to do this experiment I am