Aim-
• To investigate the osmotic effect of changing the concentration of sucrose solution; distilled water, 20% sucrose solution, 40% sucrose solution, 60% sucrose solution on the change in mass of potato cylinder after 30 minutes of being in solution.
Introduction-
Osmosis is the movement of water molecules from a region of high
Concentration to a region of low concentration through a semi-permeable membrane.
If a plant cell is places in a hypotonic solution the cell has a lower water concentration to that of the solution. Water will move into the cell by osmosis from a high water concentration outside the cell to a lower water concentration inside the cell through a selectively permeable membrane. The cell becomes turbid
If an animal
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copybook.)
Hypothesis-
If the concentration of sucrose increases, then the mass of the potato will decrease. However, if the concentration of the solution in the beaker is less than that of the potato (such as distilled water), then the mass of the potato will increase. So, as the concentration of sucrose increases the rate of osmosis increases.
Variables-
Independent variable: the concentration of sucrose solution in which the potato will be placed: o Distilled water o 20% sucrose solution o 40% sucrose solution o 60% sucrose solution
Dependent variable: the change in mass of the potato after the experiment by osmosis.
Control variable:
Temperature- all of the test tubes will be kept in room temperature
Size of potato-will be the same as it will be cut using a cork and borer which cuts them all to the same diameter.
Type of tissue- this will be controlled by using only one type of tissue, which is the potato
Materials list-
• Range of sucrose solution and distilled water
• Distilled water
• 20% sucrose solution
• 40% sucrose solution
• 60% sucrose solution
• 4 Potato cylinders
• Cork borer
• Scalpel
• 4 Test tubes
• Test tube rack
• Stop watch
• Weighting scale + plastic plate
• Ruler
• Paper
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When doing this experiment I was able to see the effect of different concentrations on the rate of osmosis, each was done by measuring the initial mass and length of the potato cylinder and after osmosis, the results were conducted to show that as the sucrose concentration increases the rate of osmosis also increases as I said in my hypothesis thusly making a direct decrease in mass.
A good example would be the experiments with the concentrations of 20% to 60% concentration results to show the change. In the 20% concentration I noticed a decrease in the percentage change in mass for the experiment the percentage was _____ while for the concentration of sucrose 60% the change was _____ we can see that the percentage change in mass is decreasing thus we can conclude that the difference of the initial from the final and as it gets greater that means the mass of the potato changed massively.
According to osmosis theory as the concentration of the sucrose solution increases the particles water potential increases and becomes higher than the particles that are in the
Investigating the Effects of Varying Sugar Concentration on the Amount of Osmotic Activity Between the Solution and Potato Tubes
Investigating the Effect of Sucrose Concentration on the Conversion into Glucose and Fructose by Invertase
My hypotheses stated that when an egg is placed in corn syrup or distilled water, osmosis will occur. They also said that when an egg is placed in corn syrup, the osmosis will occur from the water in the egg to the corn syrup while when the egg is placed in water, the osmosis will occur from water outside of the egg into the egg itself. The final part of my hypotheses states that the egg’s mass will decrease when it is placed in corn syrup, but when an egg is placed in water, its mass will increase. My reasoning when constructing my first hypothesis was that the structure of the corn syrup is too thick to travel through the cell membranes of the egg, but the water in the egg will be able to travel through the egg’s membranes and into the corn
This occurrence can be explained through the process of osmosis. The increase in mass as well as the increase in turgidity, in the potato tubes immersed in low sodium chloride concentration solutions is understood to be a hypertonic. Since the solution is hypertonic, the water molecules will diffuse into the area of lower water concentration (the potato tube) in order to achieve equilibrium. Alternatively, the decrease in mass in the potato tube submerged in highly concentrated sodium chloride solutions can be explained by its immersion in a hypotonic solution. As sodium chloride solution is less concentrated in water molecules than the potato tubes, the decrease in mass and loss of turgidity results from the net movement of water leaving the potato tube, which is higher in osmotic pressure, and diffusing into the solution.
The experiment is aimed at giving a better understatement of osmosis process and the different conditions in which osmosis occurs.
Osmosis is the facilitated diffusion of water across the cell membrane of a cell. The inside layer of the cell membrane is hydrophilic, meaning water cannot easily pass through the membrane. The cell membrane has to have aquaporins, which are water channel proteins, that move the water across the membrane. If there is a water and salt solution outside the cell, the salt can enter the cell by diffusion, but the cell membrane is not permeable to the water. Because there is more solute solution inside the cell, there is less water. The aquaporins move the water across the membrane until equilibrium is reached.
the same way as it does potato. I would also widen the range of sugar
... els of sucrose solution to distilled water (see molarity table above) and I placed each of the mixtures into the correct beakers. 5. Then I weighed all the potato chips on an electronic balance (see results) and recorded the results. 6. I placed 5 pieces of potato into each beaker and left them for approx 36 hrs. 7. After this time I drained out the solutions from the beakers and I carefully placed them in order of molarity on a paper towel. 8. I dried the potato chips gently and then weighed each potato piece and recorded the results. 9. As I had extra time I made a second experiment and also recorded those Results: Molarity (M) Starting Weight (g) (Results 1) Final Weight (g) (Results 1) Percentage Change (%) (Results 1) Start Weight (g) (Results 2) Final Weight (g) (Results 2) Percentage Change (%) (Results 2) These values clearly support my prediction, and even though there are some anomalous results, there is an overall negative trend across the whole set of results, proving them accurate. Analysis of Results: The sucrose concentration of the solution into which the potato tissue is placed affects to what degree it grows or shrinks. As you can see from the graph the results show a clear negative correlation, a very obvious inversely proportional trend. From this a conclusion can be drawn. When the water concentration is high, the potato gains water, as seen by the 0.0 molarity solution. When the water concentration is low, the potato loses weight and therefore decreases in mass, as seen in the 1.0 concentration solution. This proves my hypothesis correct. The results were quite widely ranged, as seen on the graph, wit ......
The materials needed are three small beakers (150 or 250 ml), a potato, a knife to cut the potato into pieces, a ruler to measure the potato, something to weigh the potato pieces, a timer, a calculator, and three solutions: distilled water, 10% sucrose, and 50% sucrose. The point of this experiment is to calculate the percent change in the mass before and after soaking the potato in the three different solutions. Create your own hypothesis before beginning the experiment. My hypothesis is that the potato soaked in water will have a higher mass after soaking, and that the potato soaked in the 10% sucrose and 50% sucrose will remain the same. Make sure to keep up with your measurements since they are needed to determine the mass percent change. The best way to accomplish this is to use the table provided at the end of this sheet to record your results. The first thing you need to cut the potato into three pieces of about two cubic centimeters (cm^3) in length. The second step you need to take is weighing each potato piece and writing down its mass. Next, label the three beakers with the three different solutions used. Then, you need to pour distilled water over one piece of potato, 10% sucrose on another, 50% sucrose on the last piece of potato; each solution needs to be poured on each potato piece until they are completely submerged. After they are submerged: set your timer for an
10% - 15%. I intend to place a predefined weight of potato cells (0.15g) in varying concentrations of sucrose solution (0%, 20%, 40%, 60%, 80%), to see the effects of osmosis in the cells of a potato in varying levels of sucrose solution. The potato cores will be prepared, weighed and then placed in the solution and left for a certain amount of time, they will then be removed, re-weighed and the difference in weights calc...
Investigation of the Concentration and the Effect of Sucrose on Osmosis in Apple and Potato Tissues
water in the potato, then the water will go out of the potato and into
potato cells. In order to find the best way to do this experiment I am
The variables I could change would be the temperature of the solution. The higher the temperature of the solution the more osmosis would occur. The surface area of the potato strip, the larger the surface area the more osmosis would occur as there would be more area exposed for osmosis to take place. The concentration of the solution will affect osmosis, the higher the concentration the less osmosis would take place. The duration of the experiment also affects how much water is gained/lost by osmosis as the longer the duration is, the more osmosis would take place (osmosis would stop as soon as it reaches an equilibrium of water content inside and outside the cell.
Prediction I think that when the potato is placed in distilled water the potato mass will increase. This is because water, has, if pure, a weaker concentration than the potato, and the water molecules move in through a partially permeable membrane by osmosis. If the potato is in a high concentration salt solution, the potato mass will decrease because the potato is less concentrated than the salt solution, and the water will move through the partially permeable membrane into the stronger solution. However, if the potato mass stays the same after the experiment, this means that the water/salt solution and the potato must be of equal concentration.