Finding the Best Temperature for the Enzyme Amylase

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Finding the Best Temperature for the Enzyme Amylase Aim: The aim for this experiment is to find out the best temperature for the enzyme amylase and at what temperature of water it works the fastest. I plan to collect evidence to prove that the enzyme amylase will work best at the temperature 37ËšC. I will choose a range of temperatures two below 37ËšC and two above 37ËšC so I will be able to determine what temperature it works best at. Prediction: I think that the enzyme amylase will work the best and fastest at 37Ëš. I think this because this is at body temperature so amylase works at its best to digest large insoluble food molecules into smaller simpler and more soluble molecules to be digested. Scientific knowledge: The scientific knowledge I know is that amylase digests insoluble starch molecules into smaller soluble molecules of sugar (glucose). Enzymes are biological catalysts. They speed up the rate of a reaction without themselves being used up. Enzymes are individual proteins which controls a particular reaction or type of reaction. They are specific e.g. amylase always acts on starch. Enzymes can be used over and over again. They can work up to temperatures of 40ËšC but if the temperature goes above 45ËšC the enzymes become denatured and cannot work anymore. Enzymes have a 3D shape which has to be intact for the enzyme to function correctly. If the 3D shape is altered or destroyed the enzyme will not function and will become denatured. The 3D shape is held to together by bonds and if these bonds are broken, the 3D shape is lost and the enzyme is denatured. The optimum temperature for body enzymes is 37ËšC. Amylase will only work in an alkaline condition. Lock and Key [IMAGE] Apparatus list: Five test tubes Dimpled tile Starch solution Matches Amylase solution Pipettes (1/gp) Stop watches Splints 250ml beaker H20 bath set at 37ËšC 10ml measuring cylinder Iodine solution Thermometer
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