Lactase Test Lab Report

Data table 1
Well plate Contents Glucose concentration
A 3 drops 5% sucrose + 3 drops distilled water Negative
B 3 drops milk+3 drops distilled water Negative
C 3 drops 5% sucrose +3 drops lactase Negative
D 3 drops milk +3 drops lactase 15+
E 3 drops 20% glucose +3 drops distilled water 110 ++

Questions
B. In this exercise, five reactions were performed. Of those reactions, two were negative controls and one was a positive control. Describe which of the reactions were controls and if they provided the expected results. Use the data in Data Table 1 to support your answer.
Answer- In this exercise the positive control was solution E because it contained glucose which would test positive for sugar. The negative controls would be solutions

Describe the relationship between temperature and the enzymatic activity of lactase. Of the three temperatures tested, which is the optimal temperature for enzyme activity? Use the results in Data Table 2 to support your answer. Hypothesize how the structure of the lactase relates to the results in Data Table 2.
Answer- After conducting this experiment and collecting the data I would have to say that the optimal temperature for enzyme activity would have to be room temperature which in my experiment was thirty-four degrees Celsius. I came to this answer because the glucose test strip showed that at room temperature there was more glucose concentration that at either of the other temperatures.
Due to temperature extremes in the boiling water the enzymes could no longer function because the breakdown of lactose stopped. The cold water also hindered the breakdown of the lactose but as the water warmed the enzymes were more active which can be seen in the results for the cold water at 20 minutes
B. Describe the relationship between pH and the enzymatic activity of lactase. Of the pH values tested, which is the optimal pH? Use the results in Data Table 3 to support your answer. Hypothesize how the structure of the lactase relates to the results in Data Table