Experiment to Demonstrate the Action of Amylase on Starch Solution

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Recommended: Discussion on investigating the effect of amylase concentration on its activity

Experiment to Demonstrate the Action of Amylase on Starch Solution

Plan

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Scientific knowledge: Substances called catalysts speed up many

chemical reactions. Catalysts called enzymes control the metabolic

reactions in the body. Amylase is an enzyme; it is present in the

digestive systems of many animals. Amylase speeds up the breakdown of

long chain starch molecules. I know that the more common enzymes work

best at around 37.c as this is our natural body temperature. If this

temperature begins to rise the reactions slow down, this will continue

to happen until the enzyme is denatured. It becomes denatured at

around 60.c, this happens because the enzyme becomes misshapen. This

means that the enzyme will no longer work because it will no longer

fit. When the two join then you get the chemical reaction.

Preliminary work: I watched someone else do this experiment and saw

how it was to be done. This helped me see how the experiment worked

and how to do things correctly. I have studied enzymes so this has

helped me understand what is happening.

Apparatus.

2 test tubes

test tube rack

Timer

3 Pipettes

2 syringes

Dotted tile

Starch solution

Amylase

Iodine solution

Thermometer

Bunsen burner

Tripod

Gauze

Water bath

Procedure

[IMAGE][IMAGE][IMAGE][IMAGE][IMAGE]Two drops of iodine solution is put

into each of the dimples.

[IMAGE][IMAGE][IMAGE][IMAGE]

Two drops of starch solution are put into the first

[IMAGE][IMAGE][IMAGE][IMAGE] dimple as a control.

10ml of starch solution and 1ml of amylase is measured using the

syringe. The Starch is put into a test tube, and then the amylase is

added, and the stop clock is

immediately started. With a pipette, 3 drops of the starch and amylase

solution are added to each dimple every thirty seconds. When the

solution has turned back to the original colour of the iodine solution

the stop clock is stopped. I will take 8 results and repeat all of

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