Enzyme Activity in the Catalase

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The purpose of this experiment was to see if there would be enzyme activity that is in catalase, which is found in potatoes. How my partner and me did the experiment was very simple, we put the filter paper filled with catalase concentration in to five sets of hydrogen peroxide. We found that the more concentration of hydrogen peroxide there was the faster it would take for the filter paper to reach the top, and the lower to no concentration level of hydrogen peroxide was the slower it would go to reach the top. The results of our experiment showed us that the higher or lower level of hydrogen peroxide would determine how long it would take to float to the top. Introduction: According to the textbook Biology Life on Earth with Physiology 10th edition enzymes are biological catalysts that are made of protein, and they speed up the rate of a specific biological reaction. (Audsirk, Audsirk, and Byers 100-105) and chemical reaction rates go up without changing the chemical equilibrium between reactants and products. (Cooper, 2000). All enzymes have a pocket, which is called an Active Site where reactants, which are called substrates, were substrates could enter. (Audsirk, Audsirk, and Byers 100-105) Enzymes regulate both exergonic and endergonic reactions, and enzymes are found in almost all cells (Audsirk, Audsirk, and Byers 100-105). Enzymes are regulated by three different regulations; Allosteric Enzyme Regulation, Competitive Inhibition, and Competitive Inhibition. (Audsirk, Audsirk, and Byers 100-105). In Allosteric Enzyme Regulation a molecule that is inhibited causes an active site to change shape. In Competitive Inhibition the enzyme and substrate are binding normally, and in noncompetitive inhibition a substrate imposter ... ... middle of paper ... ...rst trial it took the filter paper filled with catalase liquid 22 seconds to reach the top of the test tube, and for our second trial it took the filter paper filled with catalase liquid 25 seconds to reach the top of the test tube. This pH had a low reaction because it had the same pH as baking soda. Overall for the experiments the higher the dosages of hydrogen peroxide the quicker reaction, and the lower to none the dosage of hydrogen peroxide, the less quick reaction. Results: Our results were about the same each time, yet some were higher than some or some had no reaction at all. Our first variable had an average of 48 seconds time to float with the maximum second being 115.9 seconds and the minimum being 0 seconds. It had a faster time rising up with 3% H2O2 because of its high enzyme concentration, and it took longer or not at all because of the low H2O2.
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