The Effect of a Catalase on the Breakdown of Hydrogen Peroxide

1121 Words3 Pages

The Effect of a Catalase on the Breakdown of Hydrogen Peroxide

Aim

To follow the progress of a catalysed reaction by measuring the volume

of gas produced as the reaction proceeds. Using the initial rates of a

series of experiments I will be able to find the orders of the

reaction with respect to enzyme and substrate. Also to find out if

concentration has an effect on the reaction when an enzyme is used to

accelerate the breakdown of hydrogen peroxide.

Introduction / Background Information.

This is an experiment to examine how the concentration of the

substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the

enzyme Catalase. In this experiment I will be using yeast as a source

of catalase.

Enzymes are catalysts which speed up specific reactions. Enzymes such

as catalase are protein molecules, which speed up a specific reaction

within the cell. They are all very specific as each enzyme just

performs one particular reaction.

Catalase is an enzyme found in food such as potato and liver, (in this

case I will be using yeast as my source) It is used for removing

hydrogen peroxide from cells. This need to be done as hydrogen

peroxide is the poisonous by product of the metabolism. Catalase

speeds up the decomposition of hydrogen peroxide into water and oxygen

as shown in the equation below.

2H2O(aq) à 2H2O(l) + O2 (g)

It is able to speed up the decomposition of hydrogen peroxide because

the shape of its active site matches the shape of the hydrogen

peroxide molecule. This type of reaction were a molecule is broken

down into smaller pieces is called an anabolic reaction.

I will be studying the effect of concentration of the catalase in this

reaction.

Hypothesis

Hydrogen peroxide will breakdown to oxygen in water in the presence of

catalase. The reaction will increase with the increasing enzyme

concentration when the molecules of hydrogen peroxide are freely

available. The more concentrated the catalase the more chance of the

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