The Effect of Temperature on the Activity of the Enzyme Catalase

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The Effect of Temperature on the Activity of the Enzyme Catalase Introduction: The catalase is added to hydrogen peroxide (H²0²), a vigorous reaction occurs and oxygen gas is evolved. This experiment investigates the effect of temperature on the rate at which the enzyme works by measuring the amount of oxygen evolved over a period of time. The experiment was carried out varying the temperature and recording the results. It was then repeated but we removed the catalase (potato) and added Lead Nitrate in its place, we again tested this experiment at two different temperatures and recorded the results. Once all the experiments were calculated, comparisons against two other groups were recorded. With this information we were able to identify any patterns and similarities. Hypothesis: The higher the temperature of water, potato and H²O², the rate at which the Enzyme will work will be faster therefore producing more oxygen. The reaction will be the same without the catalase (potato). Therefore in both experiments the Enzyme will work more rapidly and produce more oxygen. Aim: To test the hypothesis. Method: [IMAGE] Equipment needed: Ruler Measuring Cylinder Scalpel Tongs Pipette Thermometer Tri-pod Stop-clock Gauze Delivery Tube Bunsen Burner Beaker Matches/Lighter Potato Hydrogen Peroxide Solution (20%) Water Lead Nitrate The skin of the potato was removed using a scalpel and then cut into 1cm², using a ruler to measure the size of each cube, four cubes are required for each experiment, and therefore at least 36 cubes are required for the full experiment to take place. Fill a beaker half way with water, and place a thermometer in the water. Allow the thermometer to warm to room temperature to gather an accurate reading, and measure the temperature, using the thermometer. A measuring cylinder was used to put 10ml of Hydrogen Peroxide Solution into a

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