Flavonoids Essay

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2.2 Flavonoid in Pharmaceutical
Flavonoids are widely distributed in plants, fulfilling many functions. Flavonoids are the most important in plant pigments for flower coloration, producing yellow, red, or blue pigmentation in plants designed to attract pollinators. In higher plants, flavonoids are involved in UV filtration, symbiotic nitrogen fixation and floral pigmentation. They may also act as chemical messengers, physiological regulators, and cell cycle inhibitors. Flavonoids, which are polyphenolic compounds, are a class of plant secondary metabolites possessing a broad spectrum of pharmacological activity including anti-cancer activities. Among the various natural products, flavonoids have attracted much attention due to their remarkable spectrum of pharmacological activities such as antioxidant, antimutagenic, antibacterial, antiangiogenic,anti-inflammatory, antiallergic, modulators of enzymatic activities and anti-cancer activity. Flavonoids have been found act as protein kinase inhibitors which can prevent alterations in phosphorylation that result in uncontrolled cell division, inhibition of apoptosis, and other abnormalities. Flavonoids can be known as angiogenesis agents. Angiogenesis is a physiological process resulting in formation of new blood vessels from pre-existing vessels. Antigiogenesis cause rapid growth of solid tumor and tumor metasta-sis are promoted by uncontrolled tumor angiogenesis. The flavonoids can control this growth of tumor. Besides that, flavonoids also can act as antioxidants which known as substances that delay, prevent or remove oxidative damage to a target molecule (Ravishankar et al., 2013).

2.3 Flavonoid in extraction
Flavonoids are universal within the plant kingdom and they are the most ...

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...tioxidants and free radical scavenging. Different sample preparation techniques such as SFE extraction, pressurized hot water extraction and solid- phase extraction have also been utilized prior to analysis. High-speed counter-current Chromatography has been used for the preparative separation and purification of baicalin and wogonin from Scutellariae radix. Crude baicalin was obtained by extraction with methanol with water (70:30) from Scutellaria baicalensis. The separation was performed in two steps with a two phase solvent system composed of n-butanol with water (1:1). In which the lower phase was used as the mobile phase at a flowrate of 1.0 ml/min in the head to- tail elution mode. The simultaneous separation and purification of active components in Scutellaria baicalensis can be analysed by High-speed counter-current Chromatography (Sujuan Wu et al., 2005).

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