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Importance of gram staining
Quiz on gram staining
Quiz on gram staining
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Gram Staining
What is gram staining?
Gram staining can also be called Gram’s Method because of the biologist it was named after, Hans Christian Gram. It is a procedure to make bacteria easier to see under a microscope
(as they are transparent) and also to differentiate between the two types of bacteria cells based on the composition of their cell wall. These two types are called Gram-positive and Gramnegative.
Gram-positive cells will appear purple and Gram-negative will appear red after the staining process.
What is involved in Gram staining?
The staining process has many steps in which the bacteria on the slide is stained so that biologists can differentiate the bacteria and therefore name the bacteria and see it clearer.
Many things are
The Gram positive bacteria has been nicknamed Posi. The Gram positive species’ morphology includes having an opaque opacity with a smooth margin. The moisture content of the Gram positive species is shiny and the pigmentation is gold. The Gram positive species grows at an optimal temperature of 37°C. The shape of the Gram positive species is a cocci, with an arrangement of grapelike clusters. The Gram positive species’ size ranges from .5-1.5 µm. Oxygen requirement of the Gram positive species is facultative, and has complete lysis of red blood cells. All results are summarized in Table
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
electrophoresis. The way the PCR method works is by first mixing a solution containing the
Phenotypic methods of classifying microorganisms describe the diversity of bacterial species by naming and grouping organisms based on similarities. The differences between Bacteria, Archaea and Eukaryotes are basic. Bacteria can function and reproduce as single cells but often combine into multicellular colonies. Bacteria are also surrounded by a cell wall. Archaea differ from bacteria in their genetics and biochemistry. Their cell membranes are made with different material than bacteria. Just like bacteria, archaea are also single cell and are surrounded by a cell wall. Eukaryotes, unlike bacteria and archaea, contain a nucleus. And like bacteria and archaea, eukaryotes have a cell wall. The Gram stain is a system used to characterize bacteria based on the structural characteristics of their cell walls. A Gram-positive cell will stain purple if cell walls are thick and a Gram-negative cell wall appears pink. Most bacteria can be classified as belonging to one of four groups (Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci, and Gram-negative bacilli) (Phenotypic analysis. (n.d.).
During this time, it could only be used in a lab with semi-intense supervision. Now, fast forward a few decades and there are D.I.Y. at home kits. The process of Electrophoresis starts with an electric current being run through a gel containing the molecules of interest. The molecules will then travel through the gel in different directions and speeds, based on their size and charge, allowing them to be separated from each other. Dyes, fluorescent tags, and radioactive labels can all enable the molecules on the gel to be seen after they have been separated. Because of these identification markers, they appear as a band across the top of the gel. Electrophoresis can be used for many different things. It is used to identify and study DNA or DNA fragments, and helps us to better understand the molecular components of both living and deceased organisms. Electrophoresis can also be used to test for genes related to specific diseases and life altering diagnoses such as Multiple Sclerosis, Down’s Syndrome, kidney disease, and some types of cancer. Electrophoresis also plays a major role in the testing of antibiotics. It can be used to determine the purity and concentration of one specific type of antibiotic or several general antibiotics at a time. Electrophoresis is also extremely useful in the creation and testing of
Bacteria play a large role in our health, the environment, and most aspects of life. They can be used in beneficial ways, such as decomposing wastes, enhancing fertilizer for crops, and breaking down of substances that our bodies cannot. However, many bacteria can also be very harmful by causing disease. Understanding how to identify bacteria has numerous applications and is incredibly important for anyone planning to enter the medical field or begin a career in research. Having the background knowledge of identifying an unknown bacteria may one day aid healthcare professionals diagnose their patient with a particular bacterial infection or help researchers determine various clinical, agricultural, and numerous other uses for bacteria.
Cells are grouped into three categories namely plant, animal, and prokaryotic cells. In addition, the shape and size of cells range from a few millimeter to microns. The size of a cell is indicative of its function(s). The shape of cells in living organisms may range from concave, to spherical, oval, rectangular, flat, oval, or rod-shaped. The cells can be viewed with the aid of a microscope. Every living organism possesses multicellular and unicellular cells. At the same time, the different types of cells display common structural properties. Examples include the plasma membrane and genetic composition (Jan,
As it became my hobby to study quite a few microscopic and gross preparations for hours every day. Working under a fine supervision of my pathology professor Dr. Bekhtereva, made me aware of my ability to identify and follow a specific pattern in a slide. My mentor emphasized how important it is to be able to combine this innate visual ability with rigorous scientific
The first thing to consider is the process of cleaning and coating of the boards. This particular process involves the set-up of the machines, the loading of the boards, and the actual cleaning and coating of the said boards by the machines.
The Wright stained lymphocytes were dark pink and pale pink in color. The size of the cells varied, some were quite large, and others were smaller. Some were clumped together in "swirls" and "lines" of cells, while other areas of the slide looked like a polka dot pattern.
- can be used to stain cell nuclei red (the cytoplasm will be unstained or yellow)
The abnormal presence of bacterial growth can be inspected under a microscope. If the organism inspected is not the bacteria used in the experiment, it means that the growth of the bacterial culture investigated is absent. By using this method, contamination by foreign substances in the surrounding air can be ruled out and the results would be more accurate.
Staining is used in a variety of ways in order to color the background of a cell, discern types of cells and to discern structures of a cell. A differential stain is when multiple dyes are used to stain a cell that take advantage of chemical differences in a cell. Gram staining is a type of differential stain that works by distinguishing gram positive and gram negative cells by coloring them violet or red, respectively. Gram positive cells contain a thick cell wall of peptidoglycan and a single membrane. Gram negative cells contain a thin cell wall which is located between two membrane layers. There are four reagents used in gram staining which include crystal violet, iodine, ethanol and basic fuchsin. Crystal violet is a primary methyl
The primary pitfall of the using the Gram-stain technique is human error. During this 4-step process, it’s easy to overheat during fixation, over wash or