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     GRAM STAINING  Gram staining is common techniques consumed to distinguish two huge groups of bacteria depend on their various cell wall contents. The gram staining procedure differentiates among gram negative and gram-positive groups by colouring these cells violet or red. Gram positive bacteria stain violet because they having thick layer of peptidoglycan in their cell wall. Which contain the crystal violet these cells are stain with, instead of gram negative bacteria stain pink or red, which

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    Gram Staining Essay

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    Introduction Staining is used in a variety of ways in order to color the background of a cell, discern types of cells and to discern structures of a cell. A differential stain is when multiple dyes are used to stain a cell that take advantage of chemical differences in a cell. Gram staining is a type of differential stain that works by distinguishing gram positive and gram negative cells by coloring them violet or red, respectively. Gram positive cells contain a thick cell wall of peptidoglycan

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    Gram Staining

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    The samples unknow bacteria from cut toenail swabbed and inoculate a tube of Tryptone Soy Broth (TSB). The tube at 30o C for 24 to 36 hours with shaked. When it passed 24 to 36 hours then perform Gram stain to find identify Gram positive and negative. Obtain a BBLTM Gram slide with ten squares with two different squares marked C for control of Gram positive and negative. Smear a small amount of bacteria from toenail in TSB into squares #1 and waited for the slide to completely dry. Then added a drop

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    Introduction Gram staining was developed by Christian Gram in the 1800’s, a Danish bacteriologist. (Smith and Hussey, 2005) It was the first differential staining technique and most common used in microbiology. Furthermore, bacteria are transparent and cannot be seen through the microscope. For that reason, Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on

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    After selecting the unknown sample # 2, the gram stain technique was performed in order to correctly identify the microbes. Gram staining is considered a differential media because staining differentiates between gram negative and gram positive bacteria. The purpose of the gram stain is to give crucial information as to whether the bacteria has a positive or negative cell wall, provides rapid results to physicians, and identifies the shape and arrangement of the organisms. The gram stain of sample

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    bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive. Introduction The purpose of this lab was to view the different characteristics of bacteria by applying various staining techniques. It is important to know the

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    This technique has a very little contrast which is mostly provided by staining specimens. • LIGHT MICROSCOPY It is the technique that uses visible light as a source of illumination to view small objects which are in finer detail than the ability of naked eye. HISTORY Antony van leewenhoek invented simple microscope in 1670

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    Essay On Teeth Staining

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    Bad Breath and Teeth Staining Halitosis is the medical term for Bad Breath. When people think of bad breath they automatically think that food is the cause of the bad odor. When in reality there are many factors that can lead up to bad breath. Yes food is one of them but there are other reasons as to why a person may be experiencing bad breath or teeth staining. A person may experience bad breath or teeth staining because they may have a health problem that is causing the odor other factors are

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    3 tests to identify Escherichia coli

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    In order to identify an unknown bacterium a variety of tests can be performed. The unknown bacterium that underwent a few of these tests was determined to be Escherichia coli. A Gram stain, citrate utilization test using Simmons citrate agar, and a urease detection test with phenol red were performed on the assigned bacterium. The unknown bacterium was determined to be E. coli because the tests concluded that the specimen was Gram-negative, bacilli, citrate utilization negative, and urease production

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    Teeth Staining Essay

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    Benefits of Drinking Coke?, 3 Oct. 2017, www.livestrong.com ›+… ›+Diet+and+Nutrition ›+Beverages+and+Health. Accessed November 7, 2017 VanVoorst, Jenny Fretland. Take a Closer Look at Your Teeth. The Child's World, 2014. Woudstra, Wendy J. “Teeth Staining.” How Does Coffee Stain Teeth?, 1 Feb. 2013, www.colgate.com/en/us/oc/oral-health/cosmetic-dentistry/teeth-whitening/article/sw-281474979067209. Accessed November 7,

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