Identifying An Unknown Bacterium From A Mixed Culture, By Conducting Different Biochemical Tests

Tesfahunegn Z, Asrat D, Woldeamanuel Y, Estifanos K (2009) Bacteriology of surgical site and catheter related urinary tract infections among patients admitted in Mekelle Hospital, Mekelle, Tigray, Ethiopia. Ethiop Med J. 47(2):117-27. Vegas AA, Jodra VM, García ML (1993) Nosocomial infection in surgery wards: a controlled study of increased duration of hospital stays and directs cost of hospitalization. Eur J Epidemiol.

Microbiology is the study of microscopic organisms such as virus, Protista and bacteria. It’s important to know and to identify what kind of bacteria and how we can treat it since is found everywhere in society. Also to know what kind of bacteria it is by the performing different biochemical test and be able to differentiate the bacteria. This is use in the medial field where is important to know what kind of bacteria there dealing with and know how to treat it. The purpose of the study was to identify what are unknown bacteria by applying all the methods that we have learn in microbiology for the identification of are unknown.

Introduction The objective of this lab was to identify unknown bacteria culture by using various differential tests. There are many reasons for knowing the identity of microorganisms including to find the correct antibiotic to treat infections the bacteria may have caused. All the methods and techniques used to identify unknown bacterium #79 was learned in the microbiology laboratory. Test Result Unknown# 79 was grown on TSA slant in a 370 c temperature after two days, the microbe replicated to more than 100 microorganisms, which has cream pigments, and convex elevations. The microbe entire margins and convex elevations.

After selecting the unknown sample # 2, the gram stain technique was performed in order to correctly identify the microbes. Gram staining is considered a differential media because staining differentiates between gram negative and gram positive bacteria. The purpose of the gram stain is to give crucial information as to whether the bacteria has a positive or negative cell wall, provides rapid results to physicians, and identifies the shape and arrangement of the organisms. The gram stain of sample # 2 reveals that one of the unknown microbes is a gram negative bacillus/rod and the other is a gram negative coccus. The EMB plate was used to isolate the gram negative rod because the EMB plate is selective for gram negative rods, inhibiting the growth of gram positive organisms.

Microbiology is the biological study of organisms that are too small to be seen with the naked eye (1). Microbiology includes examining a variety of different microbes such as bacteria, archaea, protozoa, fungi and viruses (1). By examining these microbes, their relationship with people and the environment they grow in, is better understood. These examinations of microbes have played a major role in comprehending how microbes can be helpful and harmful, and how they should best be dealt with. With new and emerging discoveries in microbiology, medical treatments have expanded.

Enteric Fever-Like Syndrome Caused by Raoultella ornithinolytica (Klebsiella ornithinolytica). Journal of Clinical Microbiology, 47(3), 868-869. Nakasone, E. S., Kaneshiro, R., Min, K., & Tokeshi, J. (2015). Emergence of Raoultella ornithinolytica on O'ahu: A Case of Community-acquired R. ornithinolytica Urinary Tract Infection.

In this experiment, the most studied bacterium, Escherichia Coli, is utilized as a model bacterium for genetic transformation. E. Coli is a model bacterium since it can extensively be studied to understand a specific phenomenon. The benefits of E. Coli refer back to the observation that it can survive varied conditions; is a single-celled bacterium; is able to rapidly reproduce and grow; most E Coli strains are harmless; E Coli can easily be genetically transformed. Diving more in depth in this experiment, genetic transformation can occur through projectile bombardment, electroporation or heat shock. Genetic transformation requires the availability of technology in order for a cell to take up and express a foreign DNA, from another organism

The isolation of Microbes in a laboratory and clinical settings are of the up most importance and due to the isolation of different microbes from each other various procedures become open for the researcher to use like six fold dilutions that allow for the quarantined microbe to be reduced down in number to ensure that the test are being run are being tested on just a single type of organism. But before dilutions can occur the microbe must first be liberated from the soil and streaked onto an Agar plate to grow. There are various methods that can be used once the organism has grown onto the agar plate to ensure that the sample collected would o... ... middle of paper ... ...actors due to the large or small amount of colonies present on my agar plated media. This could be accounted for by stating that there was a very large amount of microbes present in the soil and the dilutions did not dilute enough of the microorganism out or human error could also account for Not having any statistically significant plates to use. The bacterium that I replated was obtained from the YPD 10-3 media and it gave rise to bacterium 1 & 3, The other bacterium that I obtained came from LB 10-5 .

Microorganisms that can produce genes to combat antibiotics survive and reproduce, and those that cannot die, leaving only the resistant bacteria. Resistance is very versatile and can come in many forms, including preventing the entry of the antibiotic, exporting the antibiotic, or producing enzymes that can degrade the antibiotic. Resistance also includes the ability to modify the antibiotic target, thereby rendering it useless. An example of the inactivation of antimicrobial drugs can be seen in the resistance of bacteria with a beta lactam ring structure; these bacteria have developed enzymes such as beta-lactamases that degrade and inactivate antibiotics targeting their ring structure. Some evidence exists supporting the claim that antimicrobial substances exist naturally in the environment, contributing to resistance; however, there is more evidence supporting the claim that overuse of drugs, agriculture, and many other human uses are feeding resistance.

It is concluded that E. coli cannot utilize citrate as its sole carbon source and it cannot convert urea into ammonia and carbon dioxide. Introduction It is important in microbiology to be able to identify an unknown microorganism. Many tests that characterize functions of bacteria have been developed to accomplish this task. The unknown bacterium that was tested was discovered to be Escherichia coli. E. coli is a Gram-negative, bacillus bacterium.