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Effect of osmosis in plant
Effect of osmosis in plant
Effect of osmosis in plant
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Investigating the Effect of Sugar Solution on the Weight and Size of Potato Cells
Aim:
To investigate whether the different concentration of sugar solution
will affect the weight and size of the potato cells.
General background information:
Osmosis is defined as the movement of water or any other solution's
molecules from an area in which they are highly concentrated to a
region in which they are less concentrated. This movement must take
place across a partially permeable membrane such as a cell wall, which
lets smaller molecules (E.g. water) through but does not allow larger
solute molecules to pass through. The molecules will continue to
diffuse until the area in which the molecules are found to reach a
state of equilibrium, meaning that the molecules are equally
distributed throughout the cell, with no area having a higher or lower
concentration than any other hence equal.
Hypothesis/prediction:
For this particular investigation I believe that the lower the
concentration of the sugar solution in the test tube the mass of the
potato will be greater and the longer the potato cells will be. This
is because the water molecules pass from a low concentration to a high
concentration, (in the potato chip). Therefore, the chips in low
concentrations will gain mass and have a greater length height than in
higher concentrations such as 1molar of sugar solution. If there is no
gain or loss in height or mass then these will be the isotonic points
of the potato cells. The isotonic point is where the cells are not
increasing or decreasing in size and is known to be in a state of
equilibrium.
Apparatus:
1. Potato
2. Standard sugar solution (1molar)
3. Wash bottle
4. Test tubes and test - tube rack
5. Cork borer-size (4mm diameter)
6. Scalpel
7. Ruler
8. Wooden Board
9. Measuring Cylinder-size (250cm3)
10. Top-pan Balance
11. Paper Towels
Variables:
To enable fair test certain aspects of the experiment will have to be
Materials: 3 400 ml glass beakers, 3 whole tablets of Alka Seltzer, thermometer, stopwatch/timer, thermal glove, hot plate, ice bath
way. I will put each piece of potato into the 5 test tubes and add the
Osmosis Experiment Planning Aim: The main subject that I will be planning to investigate is the effects of a concentrated sucrose solution on potato cells on the basis of the Osmosis theory. Background knowledge: The plant cell and its structure To understand osmosis in detail I will need to explain the plant cell (which is the cell included in the osmosis experiment) and its cell membrane. Below I have a diagram of a plant cell: [IMAGE] Osmosis is about the movement of particles from a higher concentrated solution to a lower concentrated solution to create an ethical balance via a partially or semi permeable cell membrane. Osmosis in simple terms is the exchange of particles between the cytoplasm inside the cell and the solution outside the cell. What makes this exhange is the partially permable cell membrane.
I blended on high to make the potatoes more liquid-like. I grabbed the cheesecloth and placed on the top of the blender. I poured the potato extract on the container and labeled it. I found out that I have to make 1% sugar solution so I grabbed the sugar and measured into 5 grams on the scale. I added 5 grams of sugar on 250 ml graduated cylinder and poured the water into the cylinder. I mixed the sugar with water and poured it into the saucepan. I refilled the water into the graduated cylinder and poured into the saucepan. I turned on the heat of the stove and saw the sugar dissolved. I poured into a container and labeled 1% sugar solution. I repeated the same thing with 1% salt solution by using 1 gram of salt and filled the water into graduated cylinder by 100 ml. I answered question three. In the first experiment, I grabbed four transfer pipets and used it to put solutions into the test tubes by 3ml. I labeled it and placed into the plastic cups so it can stand upright. I grabbed each test tube and poured 2 ml of catalase solution into it. I also tapped and swirled to measure the bubbles by using the ruler. I wrote the numbers into the lab report. In the second experiment, I labeled the room
Osmosis in Potato Tubes Osmosis: Osmosis is the movement of water molecules through a semi-permeable membrane from a high concentration to a low concentration. Diagram: [IMAGE] [IMAGE] Aim: To see the effects of different concentration of sugar solution on Osmosis in potato tubes. Key factor: In the investigation we change the sugar solution from: 0%-10%-20%-30%-40%-50% this is the independent variable; the dependant variable is the change in mass. Prediction: I predict that all the potato tubes in pure water or low concentration sugar solution will swell because water enters their cells by osmosis.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
Influence of Temperature on the Activity of Potato Catalase Hypothesis That the higher the temperature the higher the reaction rate of potato catalyse to a point were denaturing occurs in the enzyme and the reaction rate of the potato catalase drops off. Prediction The rate of Catalase activity will be faster at higher temperatures until a point, because at higher temperatures there are more chances of collisions between the enzyme's (Catalase) active site and the substrate (hydrogen peroxide). However the rate depends on the active site being able to join with the substrate, and at higher temperatures the enzyme can be denatured, which changes the shape of the active site which thus prevents the reaction from happening. At first, as the temperature increases the activity of the Potato catalase also increases this is because the collision rate of the enzyme with the hydrogen peroxide is increased.
The Effect of Water Concentration on the Mass of Potato Tissue Aim: The aim of this investigation is to discover the effect of water concentration on the mass of potato tissue, and also to investigate the movement of osmosis through potato tissue. Scientific Knowledge: When a substance such as a sugar dissolves in water, the sugar molecules attract some of the water molecules and stop them moving freely. This, in effect, reduces the concentration of water molecules.
cork borer and a ruler. I will keep the potato chips the same size in
When doing this experiment I was able to see the effect of different concentrations on the rate of osmosis, each was done by measuring the initial mass and length of the potato cylinder and after osmosis, the results were conducted to show that as the sucrose concentration increases the rate of osmosis also increases as I said in my hypothesis thusly making a direct decrease in mass.
At point A the graph shows that no change in mass, of the potato, would have have occurred had we used a 0.2 (m) sucrose solution. This suggests that the concentration of water inside the potato would have been equal to the solution outside the potato. At point B (plain water), there is no indication that the cell is increasing in mass. This is because the cell is fully turgid and no more water can enter.
repeat the process 3 times in total to ensure a fair test. At the end
= == In my investigation to find out how salt solution concentration affect the mass of potatoes, I will investigate how much the mass of a potato changes if I leave it in a beaker of water with a specified salt concentration for half an hour. I will change the salt concentration after each experiment. Background Knowledge --------------------
The Effect of Solute Concentration on the Rate of Osmosis Aim: To test and observe how the concentration gradient between a potato and water & sugar solution will affect the rate of osmosis. Introduction: Osmosis is defined as, diffusion, or net movement, of free water molecules from high to low concentration through a semi-permeable membrane. When a substance, such as sugar (which we will be using in the experiment we are about to analyse), dissolves in water, it attracts free water molecules to itself, and in doing so, stops them from moving freely. The effect of this, is that the concentration of (free) water molecules in that environment goes down. There are less free water molecules, and therefore less water molecules to pass across a semi-permeable membrane, through which sugar molecules and other molecules attached to them are too big to diffuse across with ease.
there would be no flow of water into or out of the cell so the cell