Do nanoliposomes/chitosan blend scaffolds have cyto-friendly architectures for mesenchymal stem cells culture?

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Chitosan thin films, elaborated by solvent casting, have been functionalized by incorporate nanoliposomes based on natural vegetable and marine lecithin. The physical-chemistry properties were characterized by water contact angle, swelling kinetic test, and Torsional Harmonic Atomic Force Microscopy analysis (TH–AFM). The surface wettability, swelling ratio, roughness and local stiffness of the chitosan thin films can be modifiedand controlled by adding of nanoliposomes. The water contact angle decreases from 75° (±2°) to 50° (±1°) and 38° (±1°) when the amount of soya and salmon nanoliposomes increased in chitosan films, respectively.At the same time, the films attain equilibrium state after seven daysin the presence of phosphate buffer saline. The surface topography of films are identical in terms of asymmetry and amplitude distribution of roughness measurements but showed a significant increase of asperity height when incorporating soya nanoliposomes. This variation induces a decrease of contact rigidity (0.6 Gpa) and of adhesive force value (11.7 nN). One of principal objective is to understand the underlying cell-matrix interactions. For this purpose human mesenchymal stem cells (hMSCs) were seeded on the films, the results of in vitro biocompatibility analysis showed that nanoliposomes/chitosan blend thin films are not toxic after 24 h cells seeding. The filmsfavour the cell adhesion and proliferation when the soya and salmon nanoliposomes concentration inferior to 2 mg/mL and 4 mg/mL, respectively. The salmon nanoliposomes/chitosan blend films especially showed a best cell proliferation in hMSCs culture with the concentration of 1 mg/mL. Nanoliposomes functionalized chitosan thin films on certain concentrations could offer ... ... middle of paper ... ...w surface energy like polymers. The Owens-Wendt theory divides the surface energy into two components: one due to dispersive interactions and one due to polar interactions 46. Owens and Wendt proved that the total surface energy of a solid, can be expressed as the sum of contributions from dispersive and polar force components. These can be determined from the contact angle, θ, data of polar and non-polar liquids with known dispersive and polar parts of their surface energy, via the following relations: For these measurements, two liquids (water and diiodomethane) were used. The same Digidrop meter apparatus was used for surface energymeasurement of chitosan and blend films. The total surface energy, polar component and dispersion component of chitosan and nanoliposomes blend thin films before had been determined from the water and diiodomethane contact angle.

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