Cortisol Experiments

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In a third experiment ninety-seven undergraduate participants volunteered and of them fifty-nine were women and thirty-eight were men. Fifty-three of them were in the stress group and forty-four were in the control group. Participants with surgery and medical conditions where stress inductions would be inadvisable were excluded from participation. During session one, participants sat in a small testing room that contained a chair and a desk with a computer. The computer presented the words in each list for one second each, and the order of lists and words within lists was randomised. There was a forty-five- second break between lists. After the final list, the computer instructed participants to inform the experimenter that they …show more content…

To reduce variability due to diurnal changes in cortisol, all participants were tested between the hours of 12 and 5 p.m. Participants were contacted via e-mail the day before the experiment and asked to refrain from drinking alcohol for 12 hours prior to the experiment and from eating a large meal or consuming products with high dairy, acidity, or caffeine 60 minutes prior to the experiment, as these factors have been shown to influence cortisol samples (King, Munisamy, de Wit, & Lin, 2006; Lemmens, Born, Martens, Martens, & Westerterp-Plantenga, 2011; Lovallo et al., 2005; Nicolson, 2007; Witbracht, Van Loan, Adams, Keim, & Laugero, 2013). After signing the consent form, participants washed their hands and rinsed out their mouths with water. Participants then placed a synthetic salivette swab under their tongue for two minutes to saturate the swab with saliva. They then placed the swab in the salivette tube, which was stored at 208C until it was assayed. As menstrual cycle and oral contraceptive use has been shown to affect cortisol reactivity to stress (see Kudielka & Kirschbaum, 2005, for a review), women were asked additional questions (such as the date of their last menstruation, typical menstrual cycle length, and oral contraceptive use) to determine menstrual cycle stage. Next, participants entered a small testing booth. A …show more content…

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