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The Effects of Concentration of Sugar on the Respiration Rate of Yeast Investigating the effect of concentration of sugar on the respiration rate of yeast We did an investigation to find how different concentrations of sugar effect the respiration rate of yeast and which type of concentration works best. Respiration is not breathing in and out; it is the breakdown of glucose to make energy using oxygen. Every living cell in every living organism uses respiration to make energy all the time. Plants respire (as well as photosynthesise) to release energy for growth, active uptake, etc…. They can also respire anaerobically (without oxygen) to produce ethanol and carbon dioxide as by-products. This reaction is shown in the equation: Glucose Ethanol + Carbon Dioxide + Energy C6H12O6 2C2H5OH + 2CO2 Anaerobic respiration by yeast is generally called fermentation. Yeast is a living organism that produces enzymes. These enzymes break down glucose (by colliding with each other) to be able to respire anaerobically. I predict that the rate of fermentation will increase proportionally as the concentration of sugar increases but only up to a certain point were it will begin to decrease and eventually stop. I believe this because the more sugar added to the yeast the more glucose broken down producing ethanol and carbon dioxide. The rate of carbon dioxide produced in a minute will also increase because the higher the concentration of sugar the more heat energy produced and so the more the molecules will move around and collide. Also the higher quantity of glucose molecules the higher chance of them colliding with the enzymes. I believe the reaction will slow down and eventually stop when the sugar reaches a certain concentration because the yeast will be killed by either: 1. The high concentration of ethanol produced as a by-product. 2. The temperature of the reaction, as some of the energy produced converts into heat energy. At really high temperatures the reaction will stop because the heat will have denatured the enzymes.
In this investigation, the concentration of enzyme will be inversely proportional to the time taken for starch to be digested, until at a certain point where it will level out. It will level out because, all the substrates would have been used up, therefore there will be no more substrates for the enzymes to work on. In effect, the concentration of the substrate will act as a limiting factor. However, enzyme concentration will be directly proportional to the rate of reaction.
Investigating the Rate of Reaction between Amylase and Starch. Plan Aim: To be able to The aim of this investigation is to find out whether the volume of amylase affects the rate of reaction between amylase and starch. Prediction: I predict that the greater the volume of amylase then the faster the rate of reaction between the starch and amylase. I predict this because of the lock and key hypothesis.
Introduction / Background Information. This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. In this experiment I will be using yeast as a source of catalase. Enzymes are catalysts which speed up specific reactions. Enzymes such as catalase are protein molecules, which speed up a specific reaction within the cell.
All living organisms require energy. In order to obtain energy, cells within the organisms must go through the processes of cellular respiration and/or fermentation. The way in which “oxidation of glucose leads to ATP production” is emphasized in cellular respiration (Freeman et al., 2014).
Individual yeast cells are invisible to the naked eye, and are carried in air current. When they grow on a suitable food source (for example fruit, such as grapes and plums) they form ‘colonies’ of cells. (These can be seen as a fine white powdery film on the skins of the fruit). Yeast can feed on a variety of sugars, converting them into energy in order to grow and multiply. When it first grows, the yeast cells need a supply of oxygen in the same way a animals do when they convert sugar into the carbon dioxide and energy.
The Effect of Temperature on the Rate of Respiration in Yeast I have chosen to investigate the affect temperature has on the rate of respiration in yeast. I will use an experiment to determine whether the yeast's rate of respiration will be quicker, slower or if it does not change when the temperature is varied. Scientific Knowledge The first thing to say about enzymes is that they are proteins and they are found in all types of organisms from humans to viruses.
cork borer and a ruler. I will keep the potato chips the same size in
higher amounts of gas than vegetables and other foods. Yeast, in the presence of sugar (sucrose) and oxygen, uses aerobic respiration to create water and the gas carbon dioxide. According to the article Science of Bread Yeast-air Balloons Activity, “Yeast is tiny: Just one gram holds about 25 billion cells. That amount of fungi can churn out a significant amount of carbon dioxide, provided it has the simple sugars it uses as food” (Exploratorium, 2015). Without the presence of water, yeast can use enzymes in itself to break down the sugar, called anaerobic respiration.
Yeast Respiration Experiment Temperature (°C) [IMAGE]Number of Bubbles 10 0 20 14 30 17 40 17 50 19 60 24 70 35 80 48 Data Analysis and Conclusion The data shows a clear rise in yeast respiration as the temperature is raised. Although an optimum temperature is not evident, it can be seen that temperatures exceeding 60°C speed up the reaction. This shows the general rule that reactions become faster when the temperature is increased. Unfortunately this data does not seem to show an optimum temperature for the enzymes in the yeast to function properly, which would be expected normally.
At this level there is little activity, as there is little heat and therefore energy for successful collisions. As the heat increases so does the number of collisions and the volume of CO2 produced also increases. From the graph we can see that yeast production does not occur in a linear fashion, but behaves exponentially; as the temperature rises the rate of reaction
Cellular respiration is a process of simple reactions that allow organisms to get energy from food. Cellular Respiration requires oxygen and glucose to produces carbon dioxide, water, and energy. Since it requires oxygen, it is aerobic. The process of cellular respiration is broken into three stages. The three stages of cellular respiration are glycolysis, the Krebs’s cycle, and the electron transport chain. These stages allow for energy to be obtained in an organism. The process of the stages include breaking down food into energy molecules, breaking and rearranging molecules, and transporting them throughout the cell.
Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide
Although not shown in the fermentation reaction, numerous other end products are formed during the course of fermentation Simple Sugar → Ethyl Alcohol + Carbon Dioxide C6 H12 O6 → 2C H3 CH2 OH + 2CO2 The basic respiration reaction is shown below. The differences between an-aerobic fermentation and aerobic respiration can be seen in the end products. Under aerobic conditions, yeasts convert sugars to
This lab attempted to find the rate at which Carbon dioxide is produced when five different test solutions: glycine, sucrose, galactose, water, and glucose were separately mixed with a yeast solution to produce fermentation, a process cells undergo. Fermentation is a major way by which a living cell can obtain energy. By measuring the carbon dioxide released by the test solutions, it could be determined which food source allows a living cell to obtain energy. The focus of the research was to determine which test solution would release the Carbon Dioxide by-product the quickest, by the addition of the yeast solution. The best results came from galactose, which produced .170 ml/minute of carbon dioxide. Followed by glucose, this produced .014 ml/minute; finally, sucrose which produced .012ml/minute of Carbon Dioxide. The test solutions water and glycine did not release Carbon Dioxide because they were not a food source for yeast. The results suggest that sugars are very good energy sources for a cell where amino acid, Glycine, is not.
The process of alcoholic fermentation begins with the use of enzymes. The enzymes begin to break down the long chains in starch molecules, a polysaccharide that consists of a large quantity of glucose molecules (C6H12O6) joined by glycosidic bonds as seen in figure 1, into single glucose molecules, a monosaccharide with six carbons and five hydroxyl groups. After the starch has become sugar, the enzymes are used once again, this time to convert the sugars into ethyl alcohol and carbon dioxide, CO2, as seen in figure 2 (World of Scientific Discovery, 2007). The carbon dioxide produced is released into the atmosphere, leaving water and ethanol, the alcohol, behind. Ethanol is a colorless flammable liquid with a molecular formula of C2H6O, giving it a molar mass of 46.07 grams per mole. Ethanol is also characterized by a melting point of -114°C or 159 K.