The Beer Lambert Law

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When white light passes through a prism, it forms a continuous spectrum of colors found in the visible region of the electromagnetic spectrum. The color of the compound is a blend of wavelengths that are transmitted and not absorbed by the sample. Beer- Lambert’s Law is applied in order determine the concentration of a colored species in solution. The concentration of the colored species is directly proportional to it absorbance at a wavelength. A= εbc which A is the absorbance, ε is the molar absorptivity, b is the cell path length in cm, and c is the concentration of colored species it makes a straight line where y=mx+b (Patterson 2).

The Beer Lambert law which is applied to optics is named after Pierre Bouguer, Johann Heinrich Lambert and August Beer. Beer’s law is correlated with the aspect of light absorption through the material in which the light travels. This mathematical relationship demonstrated as:

A = εbc

Beer- Lambert’s law has applications toward the evaluation of a mixture by the application of spectrometry. This eliminates the requisite for the extensive preparation of the sample which is being reviewed. The objective of this laboratory report is to confirm the Beer Lambert law empirically. The diverse solution with are applied in this experiment are water which is combined with food coloring (Zitewitz 17).

The Beer Lambert law demonstrates the correlation which is present between the molar absorptivity, the saturation of a material and the luminescent absorption of an object. The Beer Lambert law delineates that there is a linear relationship which is present between the saturation of a solution and the luminescent absorption of a solution. In the circumstance of the existence of a linear corr...

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...hstanding, the hypothesis which was initially proposed was confirmed graphically (Clark 1).
In the field of spectroscopy, absorbance is defined as the optical density (OD) of a substance as a logarithmic proportion of the luminescent radiation which contacts a material to the luminescent radiation which is transported through a substance (Zitzewitz 395). The λ maximum of a substance was empirically predicted by the Woodward – Fraser rules. These rules have the intention of forecasting the absorption spectrum of a material and its wavelength (Woodward 1123). Spectrophotometry is defined as the empirical and quantitative assessment of the reflection of luminescence conveyance of a substance in relation to the wavelength. Spectrophotometry normally incorporates the application of a spectrophotometer. This device assesses the robustness of the luminescence (Schwedt 16).

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