ASEPTIC TECHNIQUE
Aseptic technique is a combination of principles and practices used during experiments to prevent or reduce the possibility of unwanted microorganisms from getting into Cell cultures, sterile solutions and supplies. This is more serious when working with human cell lines. Good aseptic technique is very important and basic for successful results in cell and tissue cultures or any other experiment. When a very good aseptic technique is followed, these principles and practices provides many benefits for your cultures such as Protecting the cell line from microbial and cellular cross contamination, Preventing compromise of the cell line by misidentification, Protecting the value of your cell line, experiments and cell culture processes. So here are some aseptic techniques;
PERSONAL HYGIENE AND PROTECTION:
→ Wear clean lab coats; for additional protection in the hood use a fresh, closed front lab coat with gloves that overlap the cuffs. Protective eyewear should be used when appropriate. Lab coats used for cell culture should not leave the cell culture area.
→ wearing clean gloves during aseptic procedures because if we have dry skin and if this skin somehow gets in to your experiment, your result will be affected. This is because flakes of dry skin are loaded with bacteria. The gloves can also protect you.
→ take care when handling sharp equipment this is to avoid the risk of broken or splintered glass.
→ Hair should be tied back or covered up because hair draws dirt/dust and it can cause contamination.
CULTURE AREA:
→ clean your table or culturing area before starting; this is because microorganisms can easily attach to dust particles, so cleaning the table will help us reducing the amount of dust and dirt in the culture area. Also do not open windows or use window fans that allow in outside air during your experiment If possible, this also to reduce the amount of dirt carried inside.
→ avoid water baths if possible because wetting the outside of a bottle with contaminated water before bringing it into a use is never a good idea.
→ Flame the neck of the bottles you are using before and after pouring because this will prevent air bulb contamination getting into the tube.
→ Always use separate media bottles to reduce both potential contaminations; limits the spread of contamination and cross contamination with another cell line if the bottle of medium becomes contaminated.
→ Avoid sharing bottles of media or other solutions with class mates because this can cause cross contamination and also lack of responsibility is started from sharing with others.
Gloves worn during treatment can also cause musculoskeletal disorders if they do not fit properly. Gloves that are too loose could cause the hygienist to lose control of instruments. Gloves that fit too tightly could cut of the hygienist’s blood circulation and cause pain in the muscles. Textured gloves are recommended for use because they increase grip strength of
· Rinse out mixture in flask and leave water running to get rid of the
One study evaluates the gloving procedure in the health care field in relation to virus transfer (Casanova, 2012). After the worldwide SARS outbreak, the CDC proposed a re-evaluation of PPE protocols to minimize patient exposure to infections. Casanova’s group states that CDC’s proposed protocol was “not empirically validated.” They suggest using model systems to develop this data. A previous study using a model system is cited and Casanova suggests alternatives to CDC’s protocol derived from the cited study. “One such alternative is double-gloving… When removing PPE, the outer pair of gloves is removed first, followed by the rest of the PPE items, and the inner pair of gloves is removed last” (Casanova, 2012). Casanova’s group used the model system to evaluate the viability of this double-gloving procedure. This study concluded that double-gloving does decrease the risk of disease transmission and suggests incorporating this into PPE procedures for health care workers.
After the addition of the media, we insert an aeration tube inside and cover the lid with a cotton plug and start giving them aeration. This preparation has to be put on for 3 days under proper sunlight and 25-30 degree Celsius to observe if the culture is healthy/ potent or not depending on the color each culture portrays (The nanochloropsis culture should have a grass-green color to be seen as potent and the isochrysis culture should have a dark brown color to be seen as potent), if the colors seem dull and light, then that might mean that the culture is impotent.
The extern washing her hands before setting up the examination room is important because she will be touching sterilized equipment, and in order to prevent cross contamination, gloves and hand hygiene is not only important but necessary.
There are several benefits to double-gloving that have been supported by recent studies [1-4]. By double-gloving, it has been shown that the transfer of virus to healthcare workers’ hands is significantly decreased, as compared to single gloving [1]. Perhaps the incidence of nosocomial infections could be reduced if double-gloving were a requirement in healthcare settings. In the operating room (OR), double-gloving has been shown to provide superior protection against potential exposure to blood-borne pathogens [2]. Breaking the barrier between an OR nurses’ hands and the external environment was shown to occur in 8.9% of cases in one study in which single-gloving was practiced during surgical procedures [2]. Remarkably, in the same study, it was shown that when double-gloving was practiced, there was not a single case of both layers of gloves being perforated [2]. From the evidence provided in that study, one could assume that when double-gloving is practiced, it is virtually impossible for the hands of a healthcare worker to become exposed to the external environment during a sur...
More than 375,000 nail technicians face possible health hazards everyday (“Health Hazards”). Today’s salons are investing in the latest products. Sanitation is the most important in any salon to prevent disease or injury to cosmetologists and for clients. The ingredients in these hair products are becoming stronger for some clients to handle. Cosmetologists need to understand how to keep ourselves and our clients safe. Tools, implements, and cleaning furniture is the most important. Customers are always going to be coming in and out of the salon. Cosmetologist will not always know who the person is in the salon. Wash and sanitize your hands before and after a client. Wearing personal protective equipment (PPE) will also help prevent disease (Frangie). To prevent disease and bacteria, cosmetologists need to sanitize the tools and furniture before and after they give a service to a client.
There is a reason that meticulous hand hygiene is a standard of care for all patients-it saves lives. Hand hygiene is basic task that has many benefits. It is common knowledge that hand hygiene is a “good” habit. Knowing that information, we must now explore how well nurses and other health care professionals are adhering to the standards of appropriate and effective hand hygiene practices.
There can never be enough emphasis on its teaching in school, communities, colleges, hospitals, clinics etc. There are a lot of journal articles, books and research material available to support this easy prevention technique. Rachel Barrett and Jacqueline Randle (2008) in their journal, Hand hygiene practices: nursing students perceptions, state that “Hands of healthcare workers and patients are therefore a primary cause for the transmission of infections by both endogenous and exogenous spread” (Para. 1). This is a relevant point which must be highlighted in every study material and teaching plans by the tutors in healthcare settings. Without proper hand hygiene it not possible to decrease the ill effects of infections spread through contact. The authors further discuss that other standard precautions should be kept in mind as those are important too. For example, while doing dressing change or assessing the pressure ulcer it is crucial to wear gloves as hand washing alone would not be enough to protect from contamination. However, the main point to remember is that gloves should not be used as a substitute for hand
Comment on class result with respect to differences in filter types, differences in filter assemblies, and overall on the confidence you would have in using this type of sterilisation process in preparation of pharmaceutical products. List the factors that may cause contamination during filtration. (20 marks)
Animal Experimentation. Ed. Susan C. Hunnicutt. Detroit: Greenhaven Press, 2013. At Issue. Rpt. from "Avoiding Animal Testing: Advances in Cell-Culture Technologies are Paving the Way to the Complete Elimination of Animals from the Laboratory." The Scientist (Nov.-Dec. 2011). Opposing Viewpoints in Context. Web. 16 May 2014.
· If spilt on clothes or skin, wash with water to prevent harm to the
• Using mouth, nose, and eye protection during procedures or tasks that may cause splashes or sprays of blood or body fluids.
Plants are grown under controlled and sterile conditions which reduce the risk of being exposed to pests, pathogens and diseases.
In conclusion, my overall impression is that this article is very useful to understand that cross-contaminations can be control by the use of effective hand hygiene. It is not only washing hands between patients or sanitizing only because our hands looks clean, it is a matter of knowing when and why we are risking patients if inappropriate technique is used, in few words: knowledge, commitment and responsibility.