Ph Level Lab

Figure shows the pH levels that were predicted before measuring the actual pH of the different types of samples using the pH paper.

The pH level of water, 0.1 M NaCl, Skim milk, and 0.1 M PBS is measured and once the acid, 0.1 M HCL was added to these samples the pH level was measure again to determine which buffer was the most effective.

: The enzyme activity was measured for tubes 3 through 6 over the 20 minutes that they were exposed to 22 degrees Celsius room temperature, 4 degrees Celsius in ice and 50 degrees Celsius in water bath.

The enzyme activity was measured for tubes 4 through 8 depending on their pH level.

Results:

The purpose of this experiment was to gain more knowledge on the pH levels and the enzyme activity.

The pH of vinegar was 3, skim milk had a pH of 7, pH of apple juice was 4, the sprite had a pH of 4, the pH of bleach was 10, tap water had a pH of 7, pH of distilled water was 5, the pH of 10mM HCl was 2, 1.0mM HCl had a pH of 5, and the pH was 6 for 0.001mM HCl (fig.1). We wanted to know how buffers can minimize the changes in an environment’s pH. The water had a pH of 5 and after 0.1M HCl was added to the water it had a pH of 2. The pH of 0.1M NaCl had a pH of 10 and once 0.1M HCl was added the pH went down to 2. Skim milk had a pH of 7 and after the acid 0.1M HCl was added the pH was 6. The pH of 0.1M PBS had a pH of 7 and after the acid was added it had a pH of 4 (fig

The pH level was measured from all 10 tubes before the catalase solution was added to the tubes. When the catalase solution gets added to the tubes, the outcome of this solution is that bubbles are seen during this process. The bubbles are considered the oxygen production of the catalase solution. The bubbles were scaled depending on how strong the bubbles were seen. The tubes that had an oxygen production of 5 were numbered 5 through 10. Tubes 1 and 4 had no bubbles. The oxygen production of tubes 2 and 3 had a number of 1. The enzyme activity was compared to the pH level of tubes 4 through 8 to determine the activity bases on the liquids these tubes have (fig