Peroxidase Lab

Research on the Optimal Environment for Peroxidase to Survive with a focus on Temperature, pH, and Concentration
Introduction
Enzymes play a major role in virtually every single ecosystem as a conductor of reactions. They are important because without them reactions that need to take place in these cells would occur to slowly for DNA replication to occur as well as other reactions. The enzyme chosen for this experiments was peroxidase. “Peroxiredoxins (Prx), regulate the intracellular concentration of H2O2 by reducing it in the presence of an appropriate electron donor.” (Choi et al.). Enzymes are organic molecules that are made up of a string of carbons oxygens and hydrogens. The main component of bonding in these strings of molecules is

The experiment will test the reactivity of various proteins, and such is determined by the change in color of the solution. The change in color of this solution occurred due to the addition of the guaiacol dye that reacted alongside the peroxidase. First, the amount of peroxidase solution used in each test needed to be constant, so we had to determine the optimum amount of solution to use. It was important to use accurate and precise data, so pipets were used to make sure the data was consistent. Through aligning seven test tubes, each with varying amounts of the peroxidase (.5mL, 1mL, 2mL) we found at which point the peroxidase is the most productive. By adding each solution to a spectrophotometer, we were able to determine the point at which the enzyme was the most absorbent, which in this case was 2mL, and this was carried though the whole

Eleven clean test tubes were obtained, and reagents with the exact amount of buffer, hydrogen peroxide, peroxidase, and guaiacol were all mixed and spread out over the eleven test tubes in 5 segments. The Buffer and the peroxidase were in one tube while the hydrogen peroxide and the Guaiacol were in another. These were then exposed to water baths of varying interval temperatures at 4, 22, 45, 60, 100 Celsius. Once the solutions had reached their specified temperature, they were mixed together and immediately were tested with the spectrophotometer. The results were recorded in the lab notebook and were measured in