Comparing Free to Immobilised Amylase Enzyme in Its Catalysis Rate

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Comparing Free to Immobilised Amylase Enzyme in Its Catalysis Rate Method: First of all, the Immobilised enzymes need to be made. The method used to create these immobilised enzymes would be Micro encapsulation. This means that the enzyme used, in this experiment being Amylase, is encapsulated inside Sodium alginate. The enzyme was believed to act quickly, so the enzyme would have to be slightly diluted in order to get a good range of results. !0cm³ of Amylase will be added to 20cm³ of Sodium alginate, and then mixed. Using a pipette, the Sodium Alginate - Amylase mix is dropped into Calcium Chloride, and this will form the beads of Immobilised enzymes needed for the experiment. As soon as Sodium Alginate touches Calcium Chloride, it solidifies and this traps the enzyme inside. The good thing is that the beads are semi permeable, so the substrate can penetrate the bead and get to the enzyme. As I'm sure you are well aware, enzymes do not get used up in a catalysis reaction, so the beads can be used again and again to our benefits. The Immobilised enzymes will be kept in water to prevent cracking and drying out and about 110 beads will be made. The free enzyme will not take so long to prepare. The enzyme itself, Amylase had a concentration percentage of 0.125%, which is quite strong, to my knowledge. Judging from previous experiments, this has been known to work quickly, so small time intervals will be used in order to get a wide range of results. In order to keep this experiment a fair test, the free enzyme must be diluted to the same extent as the Immobilised enzyme, so 10cm³ of enzyme was added to 20cm³ of distilled water. M... ... middle of paper ... ... of enzyme in the 2cm³ as they may have been 36 beads instead of 37. Its possible that it could have got infected with bacteria, and this would have bred in the test tube, seeing as it was just over optimum. The bacteria could have got in the way of the enzyme and substrate and caused the reaction to take longer then necessary. Again I say that it is a possibility. Human error may also have caused the anomaly. Maybe too much starch was added to the test tube, or too much Buffer solution. The only way to get rid of this is to repeat the experiment 3 times and then take an average. This would have been done if given more time. Overall, the free enzyme works quicker than the immobilised enzyme at optimum conditions and the immobilised enzyme works better than the free at higher temperatures, exceeding optimum.

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