Catechol Oxidase Lab

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What is Catechol Oxidase? By: Jettica Williams BIOL 1107 Lab October 03, 2016 Prepared for Miss Fulford Introduction: During this lab we took a more in depth look at the results of catechol oxidase activity. Biological catalysts that speeds up chemical reactions and are inexhaustible are called enzymes. The substrate is the material that is being acted on by the enzyme. When it comes down to enzymes, they perform better. Enzymes work better due to the fact that they can bind to the change state better than the substrate. As this happens it can lower the beginning energy triggering the speed of a reaction to quicken. Catalysis is very crucial. Catalysis makes chemical reactions occur quicker than they would without any assistant. …show more content…

Above in table 4.1 the results are shown. Test tube 2 was the only tube that had a change in color. The reason that test tube 2 was the only one that changed in color was because a reaction that was produced by catechol oxidase. Tube 2 was the only tube that had potato extract and catechol this is why the reaction occurred. The potato extract and catechol was not present at the same time in the other tubes and that is the reason they had no change. In the second experiment 4.2, the effects of inhibitors on the enzyme catechol oxidase were observed. Phenylthiourea (PTU) was the specific inhibitor that was used. The phenylthiourea inhibits catechol oxidase by combining with copper that is a cofactor for the enzyme. The first test tube had the same amount of catechol and phenylthiourea. The reaction from the first test tube didn’t take place. In the second test tube there were 2x as much catechol as phenylthiourea. The reaction took place, but at a slower pace. There was no phenylthiourea in the third test tube, which made allowed the reaction to be accomplished fully. Works

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