Enzymes That Have A Catalytic Action Essay

Enzymes That Have A Catalytic Action Essay

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Enzymes are proteins that have a catalytic action in living organisms. A catalyst is a chemical compound that increases the rate of reaction “…without increasing the amount of products involved and without themselves being altered during the reaction”. Therefore, as enzymes are not altered during the chemical reaction, they are reusable. However, enzymes do not increase the amount of final product, they only increase the rate of reaction. For a biochemical reaction to take place a substrate must bind to the active site of an enzyme. A substrate is a molecule that an enzyme acts upon. The enzyme and the substrate have unique three-dimensional shapes, specific for their function, which allows binding of complementary enzymes and substrates. Binding occurs at the active site of the enzyme, which is complementary in shape to the substrate. If the shape of the active site is changed, and is no longer complementary, the substrate will be unable to bind to the enzyme effectively. Enzyme function may be affected by many factors, including pH, temperature and the presence of chemical inhibitors. The main factor being tested in this investigation is the concentration of hydrogen peroxide on the enzyme catalase.

〖2H〗_2 O_2 □(→┴catalase )〖2H〗_2 O+O_2

hydrogen peroxide□(→┴catalase ) water+oxygen

Equation 1: Decomposition of hydrogen peroxide into water and oxygen by catalase

Catalase is an enzyme that decomposes hydrogen peroxide into water and oxygen as seen in Equation 1. Catalase is found in animal cells and abundantly in the liver cells. Catalase helps to control the levels of hydrogen peroxide in the body. Hydrogen peroxide is a strong oxidiser and, if hydrogen peroxide levels are not controlled, it can damage the cells and the c...

... middle of paper ...

... peroxide
hot water bath
chopping board

Precisely cut 21, 1cm x 1 cm x 1 cm cubes using a ruler and scalpel
Using a 10mL measuring cylinder, measure out 3 x 10mL of 10% hydrogen peroxide solution
Add the three samples of the 10mL of hydrogen peroxide into three different 250mL measuring cylinders
Add three drops of detergent to each of the 250mL measuring cylinder
Gently swirl the cylinders and place them into a 35 degree hot water bath
Put the cylinders into the hot water bath until the inside temperature matches the temperature of the water bath
Place a piece of liver into each of the three cylinders
After three mins, take the cylinders out and observe
Every two minutes, measure the volume of foam produced in mL and record on a table
Repeat steps 2-9 using hydrogen peroxide concentrations of 0%, 1%, 2%, 4%, 6% and 8%.

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