Purpose
The purpose of this lab was to see firsthand the diffusion of a substance across a selectively permeable membrane. Diffusion is the movement of molecules from an area of high concentration to an area of lower concentration until both concentrations are equal, or as you could more professionally call it, equilibrium. This concept is one that we have been studying in depth currently in Biology class.
Procedure
We separated into groups of two each, making sure that we had the following materials for the lab: graduated cylinder, plastic sandwich bag, starch solution, twist tie, 500-mL beaker, iodine solution, and a pair of goggles and an apron (for the person handling the iodine solution).
Next we would put the starch solution, the right amount of course, into the plastic sandwich bag and secure it with the twist tie.
Following this, one person in our group would put on the protective gear (goggles and apron) to handle the iodine solution carefully. We would pour 250 mL of water into a 500-mL beaker, following with 15 drops of iodine.
After this we would place the s...
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
The procedure of the lab on day one was to get a ring stand and clamp, then put the substance in the test tube. Then put the test tube in the clamp and then get a Bunsen burner. After that put the Bunsen burner underneath the test tube to heat it. The procedure of the lab for day two was almost exactly the same, except the substances that were used were different. The
For the lab experiment of mixing the diffusion and exposure solution, we are going to test if the pigment is released in the exposure solution. We used materials as follows: Obtaining a beet, we punched out 2 cm long beets using a cork borer. Then we wash the beets in running water, after that we prepared 10 test tubes each containing different pH solution.
Specifically for the lab discussed we were to determine whether sucrose of RO H20 was of higher or lower concentration. We were able to determine permeable membranes were depending on the concentration of the solution or solvent. In this case we learned that solution and solvents of lower concentration allow for greater flow through the semi-permeable membrane, which supports the idea of diffusion. We learned that sucrose solution allowed for greater diffusion than RO H20. Also chloride ions and aluminum allowed for diffusion whereas starch and sulfate ions did not. We also learned Elodea in NaCl solution underwent a process called plasmolysis where the cells
While completing this lab we used four liquids. We used water, milk, orange juice, and soda. To hold the liquids we used two q-tips. We used two petri dishes, one was to hold the pill bugs while they were not being used in the
1) Measure out 200cm3 of water using a measuring cylinder and use a small amount of this to make a paste with 2g of soluble starch.
1. Put about 400 ml of water and only 5 to 6 ice cubes into a 600-ml beaker.
When a cell membrane is said to be selectively permeable, it means that the cell membrane controls what substances pass in and out through the membrane. This characteristic of cell membranes plays a great role in passive transport. Passive transport is the movement of substances across the cell membrane without any input of energy by the cell. The energy for passive transport comes entirely from kinetic energy that the molecules have. The simplest type of passive transport is diffusion, which is the movement of molecules from an area of high concentration to an area of lower concentration. Diffusion
In diffusion, particles move from an area of high concentration to an area of low concentration until they reach equilibrium. The direction of diffusion is called the concentration gradient. An example of diffusion would be if a person sprayed air freshener in a room. The particles would move from the area of high concentration to the rest of the room, so after a few minutes, people can smell it from the other side of the room. Osmosis is the diffusion of water across a semipermeable membrane; the molecules move from an area of high water concentration to an area of low water concentration until they reach equilibrium. Water flows in the direction to dilute. For example, a person’s cells have more salt than a poot -- it has a higher concentration of solutes. Water from the pool will flow into the body faster than the water from the cells comes out, so the cell will swell up. Both diffusion and osmosis do not require no
Then I sat the tie on the counter to the left of the towel and grab two slices of bread from the bag with my right hand. I then lay the bread down on the towel flat, side by side. Next, I hold the open end of the bread bag with my right hand and spin the bottom with my left. Then I hold the twisted part, pick up the twist tie with my right hand and tie the bread up. I lay the bread down on the counter and grab the `jar of peanut butter. I hold the jar in my left hand and unscrew the lid with my right. After unscrewing the lid, I lay it down on the counter and pick up the knife by the handle. Once the knife was in hand I scoop out some peanut butter onto my knife. I then spread it evenly onto one slice of bread until completely covered and is about an eighth of an inch thick. I repeat these steps on the other slice and sat the peanut butter jar down. Then, I grasp the edge of the paper towel with my thumb and forefinger of my left hand and wipe the peanut butter off from the bottom to the tip. Afterward, I place the knife back on the
To determine if the number of candles lit and how much the water rises underneath beaker is related.
Diffusion and osmosis in living cells are greatly effected by factors such as solution concentration, temperature, and surface area of the cell. This was studied using agar cubes of different sizes, dialysis tubing, and potato cores to demonstrate how the different factors affect cells. The process of cellular respiration can be altered due to temperature differences. Enzymes also greatly affect the molecular processes that occur. Enzymes are denatured at variety of pH and causes inactivity of the enzyme in the reactions.
Analyze each fraction by spotting 10 times with capillary tubes on a TLC plate, which is exposed to iodine vapor for 15 minutes.
Diffusion is the movement of a substance from an area with a high concentration to an area that is less concentrated (Houtman, A., et .al.,2015). However, osmosis is a special type of diffusion where water moves across a semi-permeable membrane. Understanding enzymatic activity is important when analysing the breakdown of sucrose. Enzymes are important when it comes to the function of cells. Enzymes are protein molecules that have the ability to speed up chemical reactions (Houtman, A., et.al., 2015). Invertase is an enzyme that breaks down sucrose into fructose and glucose (WOU Biology, 2018). When the large sucrose molecule is broken down into fructose and glucose, they become small enough to move through semi-permeable membranes. My hypothesis is if we put invertase in a solution with sucrose, it will catabolize it, and the glucose will diffuse across the membrane so we should get less of a rise in the osmometer. I predict that the solution that has more of a rise in the osmometer will be the one with just sucrose, the green solution.
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and