Intermediate Inorganic Lab

The sample was poured in the Buchner funnel and a about 5 mL of water was in the flask to get the last few of crystal out of the flask. After the crude aspirin dry in the beaker it weighed about 2.575 without the beaker. The final experiment, was purification. The crude aspirin was tranfer into a 125 mL Erlenmeyer flask with 3 mL of ethanol and warm on the hot plate until the aspirin dissolve. Fiver portions of about 3 mL of water was added in increments and cooled in an ice bath for about 10 minutes.

Repeat this process for a total of 10 teaspoons. Second Test In a 100ml beaker place 50mls of water, measure the temperature of the water and record this initial temperature onto a table. Set the timer and add one teaspoon of Ammonium Nitrate to the water, stir this continuously until the Ammonium Nitrate has dissolved.

(1999). Freeze-dried avocado mesocarp (0.05 to 0.10 g) was mixed with 10 mL 80% (v/v) ethanol and homogenized for 1 minute. Thereafter, the mixture was incubated in an 80oC water bath for 60 min to extract the soluble sugars. Subsequently the mixture was kept at 4 ºC overnight. After centrifugation at 12000 g for 15 min The mass of each fruit was weighed before cold storage using a weighing scale.

Briefly, 0.1 ml of the sample extract was added to 7.5 ml of distilled water, 0.5 ml of Folin Phenol reagent, 1 ml of 35% sodium carbonate solution and was diluted to 10 ml with distilled water. The mixture was shaken well, kept at room temperature for 30 min and absorbance was measured at 725 nm. Gallic acid was used as a positive control. Values were expressed in terms of gallic acid equivalent (mg/g of extracted compounds).The assay was carried out in triplicates and expressed as mean±SD. Thin layer chromatography and Bioautography: The seed extract with a concentration of 1mg/ml was spotted on the TLC plates.The solvent system used was acetone and hexane with various ratios.

Another six test tubes were labeled with different pH and enzyme source Mammal or Fungal Amylase. Add 0.5ml of amylase into the test tubes, and 0.5ml of pH buffer to each test tube. Allow the tubes to equilibrate for five minutes, then transfer a few drops of the starch solution from each pH treatment add them into the first row of wells on the spot plate. This serves as a control to make sure that the breakdown of starch is not affected by pH. Then add two drops of iodine reagent to each of these wells and wait one minute, then add 0.5ml of amylase to each of the appropriate tubes.

By comparing the polarity indexes, you can tell what solvent will separate your dots of product on your TLC plates the best (Skoog 761). Experimental Section The equipment needed for this experiment is as following: 100 mL round bottom flask, stir bar, water condenser, hot plate, UV light, Pasteur pipet, ice bath, separatory funnel, rotary evaporator, evaporating dish, cotton for filtering, and UV-vis spectrometer. The chemicals required are: 0.1 g. of H2TTP made previously, 20 mL N,N-dimethylformamide, 0.16 g. hydrated copper acetate, distilled water, 75 mL dichloromethane, hexane, toluene, ethyl acetate, dichloromethane, and acetone for TLC plates. Procedure 1. Measure out 0.1 g. of the H2TTP made previously into a 100 mL ... ... middle of paper ... ...aporates off the gel leaving open sites where polar molecules can bond.

Then add 0.8g NaOH to the cold Alclorite solution. Cool the NaOH/Alclorite solution to -10°C in the freezer. Grind 1.5 g phthalimide to a fine powder. Now add this to the cold (-10°C) Alclorite solution, plug the flask and mix with magnetic stirrer (or shake). The phthalimide will dissolve within 5 minutes.

Experimental Setup: Fermentation Broth Analysis Fermentation broth was diluted by a 1:5 dilution to 40 grams. After dilution the sample was centrifuged at 4000 rpms for 5 min. The supernatant was filter sterilized using .22micron filters. After filtering, the sample was analyzed using HPLC with a BioRad Aminex HPX-87H Column. Using this method, it was able to be determined the amount of Maltose, Glucose, Fructose, and Maltotriose in each sample.

Two quantities of distilled water were then added to this mixture to make it cool even further, which were 41 drops and 30 milliliters. After cooling for some time, this beaker was placed into an ice bath in order to start the crystallization process. A glass rod was used to scratch around the bottom and the sides to catch all of the crystallized Aspirin that was being formed during this whole process. Then, by using a Buchner funnel and filter paper, which was placed on top of the flask connected to a water aspirator with rubber tubing, the excess liquid was removed from the just scraped Aspirin crystals when the Aspirin was placed on the filter paper. Using a medicine dropper, the Aspirin crystals on the filter paper were washed with distilled water just so that any non-pure substances were removed from the crude product.

Also for mucilage extraction, Cactus stems were removed skin and cubed (1 cm3). Samples were homogenised (20% w/v) in distilled water. The slurry was centrifuge for 10 min at 4,500 rpm and the supernatant precipitated in ethanol and finally dried (Sáenz et al., 1992). Preparation of liposomal oil Multilamellar vesicles were prepared according to the thin film hydration method (Gortzi et al., 2006). Lipid solution was prepared by dissolving 5 mg/ml of phosphatidylcholine, 1 mg/ml cholesterol and 0.1 mg/ml essential oil in 3 mg/ml chloroform.