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Bioluminescence apologia marine biology modue 14
Marine bioluminescence essay
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Being able to see in the dark often does not come easy. For humans, we typically need to find a flashlight, and more times than not, find new batteries to power the device. For animals, it can be different. Some animals may see fine and night, and some animals have large eyes like owls that capture lots of light, and some animals use other senses to gather information about their surroundings. We humans on the other hand are left fumbling for candles when the power is out for any length of time. But there are some life forms that have a completely different approach – bioluminescent life forms. Bioluminescence life forms make their own light and carry it around in their bodies. This paper will address bioluminescence and try to explain it. The first point to know about bioluminescence is that there are many things still unknown about bioluminescence. Sometimes you have to seek a philosopher’s opinion on a subject so complex. Aristotle makes a valid introduction to the meaning of bioluminescence when he suggested, "Some things which are neither fire nor forms of fire seem to produce light by nature."
Bioluminescence is the biochemical emission of light by living organisms. It is a chemical reaction that burns fuel and releases light that barely produces heat- a cold light. Bioluminescence serves the three basic purposes of "finding food, finding mates, and defending against predators," says Edie Widder, co-founder, president and senior scientist at the Florida-based Ocean Research and Conservation Association (ORCA). Up to 80% of our oceanic life has the ability to produce light, and although bioluminescence is rare on land, there are still species such as fireflies, earthworms, and even bacteria that can light up thems...
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...tical profiles of bioluminescence and other physical properties. Recently, the case Lab and Cyril Johnson of the UCSB Physics Electronic Shop has developed a small version of the bathyphotometer which is an underwater vehicle. These instruments are developing every day and getting more complex, helping us understand the mysteries of the glowing creatures.
The impact of this technology and the growing research will influence and modify the future of what we now know of bioluminescence. Even today, there are more scientists creating new, more complex instruments and finding more data that we did not have access to before. There is still many things that we do not know about bioluminescence, and in the future all of these questions will be answered with the right technology and advancing research. Until then, we can enjoy nature’s flashlights - batteries not included.
Enhanced green fluorescent protein (EGFP) was originally isolated from a bioluminescent jellyfish called Aequorea victoria. As suggested by the name, this protein fluoresces green when exposed to light in the ultraviolet range. The ultimate goal of the following experiment was to successfully create a pET41a(+)/EGFP recombinant plasmid that was transformed into live E. coli cells. The success of this transformation could be evaluated based on whether EGFP’s fluorescence properties were displayed by the colony in question. The protein’s fluorescence properties “triggered the widespread and growing use of GFP as a reporter for gene expression and protein localization in a broad variety of organisms” (Ormo, et. al., 1996). Although EGFP and GFP differ for a few amino acids that make EGFP’s fluorescence mildly stronger, the basic principle that such a protein allows for the evaluation of transformation success remains intact.
al. (1994) explain that a complementary DNA for GFP produces a fluorescent product when expressed in E. coli cells as the expression of GFP can be used to monitor gene expression and protein localization in living things. In this experiment, the heat shock method will be used to deliver a vector (plasmid) of GFP to transform and grow E. coli bacteria. Four plates containing Luria Bertani (LB) broth and either –pGLO or +pGLO will have E. coli bacteria added to it. The plate containing –pGLO (no pGLO) and LB will show growth as ampicillin will be present killing bacteria but no glowing because no arabinose will be present for glowing to be activated, the same result will be seen in the plate containing +pGLO, LB and ampicillin.
A spectrum is a group of light wavelengths that are ordered in relation to their wavelength length. The electromagnetic spectrum consists radio waves, microwaves, infrared, visible, ultraviolet, X-rays and gamma rays. (1)Specifically, this lab looks at the visible light part of the spectrum because one of the colors in the visible light spectrum is shine through the sample. The visible light spectrum consists of colors of red, orange, yellow, green, blue, indigo, and violet. The color chosen to be shine through the sample is affected by the color of sample when mixed with the indicator Ammonium Vanadomolybdate (AMV). The color on the color wheel that is opposite of the solution’s color is the color that is shined through the
The very symbol of life – the elemental force of the Sun – is rendered
In 1895, Professor Wilhelm C. Roentgen, a German physicist, was working with a cathode ray tube, much like our fluorescent light bulb. The tube consisted of positive and negative electrodes encapsulated in a glass envelope. On November 8, 1895, Roentgen was conducting experiments in his lab on the effects of cathode rays. He evacuated all the air from the tube and passed a high electric voltage through it after filling it with a special gas. When he did this, the tube began to give off a fluorescent glow. Roentgen then shielded the tube with heavy black paper and discovered a green colored fluorescent light could be seen coming from a screen located a few feet away from the tube.
The plasmid pGLO contains the GFP gene which can only be activated while in the presence of arabinose. The plasmid also contains an ampicillin resistance gene, which explains why bacterial cells transformed with pGLO can survive when ampicillin is present. GFP is a protein that is found in jellyfish, and is known for emitting a green fluorescent light. In vitro, it is able to fluoresce this green color by releasing photons from being in an excited energy state. The energy was provided by a UV light at the end of this experiment. Jellyfish can fluoresce this green on their own because in vivo, GFP works with another protein that allows the GFP to fluoresce without being in an excited energy
The glass doors gently moved aside at Heath’s presence with a calm whirring hum. With it being a horrendously bright day outside, Heath found the muscles around his eyes relaxing with a sigh when he finally didn’t have to shield his vision from the sun any longer. Fluorescent lights were miles better than sunlight, naturally – fluorescent lights did not burn flesh, they did not bring stinging pain to the eyes, and they did not pound heat relentlessly into the ground in the same unforgiving way that the sun did.
The absorption of light in the form of photons through the thylakoid membrane into the lumen is the first step of photosynthesis. This photons absorbed through the lumen go through photochemical reduction in which they are absorbed into pigments such as chlor...
This experiment synthesized luminol (5-Amino-2,3-dihydro-1,4-phthalazinedione) and used the product to observe how chemiluminescence would work. The starting material was 5-nitro-2,3-dihydrophthalazine-1,4-dione, which was, after addition of reaction agents, refluxed and vacuum filtered to retrieve luminol. Using two stock solutions, we missed our precipitated luminol with sodium hydroxide, potassium ferricyanide, and hydrogen peroxide, in their respective solutions, in a dark room, to observe the blue light
The retina contains rods and cones which detect the intensity and frequency of incoming light and, in turn, send nerve impulses to the brain.
Polman, H., Orobio De Castro, B. & Van Aken, M. A.G. (2008). Experimental Study of the
Perales, J. C., Verdejo-García, A., Moya, M., Lozano, O., & Pérez-García, M. (2009). Bright and dark
An inspection of the modern animal phyla will reveal that eyes are just as diverse as they are complex. Some organisms like the rag worm have pigmented cup eyes while other like he box jellyfish have two lens eyes and two pairs of pigment pit eyes. To account for the diversity in eye structure, we must first examine the eye ‘prototype’, the original structure that was acted upon by evolution. The simplest organ that can be considered an eye is composed of a single photoreceptor cell and a single pigment cell, without any lens or other refractive body (Arendt, 2003). Such organs are know as eyespots, and...
Life according to scientists is “the condition that distinguishes animals and plants from inorganic matter, including the capacity for growth, reproduction, functional activity, and continual change preceding death, also the way of life of a human being or animal.”("Life,”) In order for one to have life, one must have the nine characteristics to be considered a living thing. These nine characteristics are; all living things are made up of cells, living things are able to reproduce, living thing use energy, maintain homeostasis, respond and adapt to the environment, grow and develop, have a life span, evolve over time, and are interdependence. All of the nine characteristics have one thing in common, something that is needed for all living things to use, even if they do not know it. This beautiful thing that all living things should value is photosynthesis. Without photosynthesis there wouldn’t consist humans, animals, insects, and most importantly life!
Light can be classified as a form of electromagnetic radiation, which includes visible light. The ‘light’ commonly referred to in everyday life belongs in this category. The electromagnetic spectrum includes other types of radiation such as gamma rays, radio waves and cosmic rays, all of which possess distinct wavelengths, frequencies and energy levels. These forms of electromagnetic radiation are not visible to the human eye but can be perceived by selected species of animals, such as bees. Figure 1 below displays the electromagnetic spectrum and provides a basic insight into the respective characteristics of different forms of radiation.