Analyzing Bacterial Strains of Bite Mark Victims

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Specific Aims:

In the United States, it is estimated that 63% of cases are solved, however that leaves 37% that are left unsolved. In violent crimes alone, the U.S. suffered over 1 million cases, of which almost half were left cold (FBI, 2010). A number of unsolved cases remain that way because of the lack of evidence, such as DNA at the crime scene. I intend to use the bacterial strains found in bite marks of victims to match the marks to the biter by comparing the strains found in each location. The bacteria amplified, using Polymerase Chain Reaction, and allow for a comparison between the various strains that should be found. From this, I will be able to suggest that bacteria found in wounds at the scene of a crime can be used to determine suspects.

There are three main points that need to brought into perspective The first is to test the saliva from each bacteria to determine that their bacterial strains aren’t the same genotype. The main bacterial type found in the human mouth is streptococcus (Kennedy 2011); assuming that is true in the cases of the volunteers, it is assumed that the bacterial strains of streptococcus found on the teeth and in saliva will be transferred to the skin of the victim as well. Though streptococcus is typically the most predominant strain found in the oral cavity, for the purpose of this study, the most predominant bacteria found in the volunteers of the experiment is what will be used in the results. The second is that the bacteria will survive long enough on the skin and survive the duration of the experiment. The last aim is to perform PCR and see if the bacteria, once amplified can be matched back to the person who it came from.

With these three points, I hope to prove that bacteria lef...

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Peipert, J.F. and Domagalski, L.R. (1994) Epidemiology of adolescent sexual assault. Obstet Gynecol 84, 867–871.

Pretty, I. A. and Sweet, D. (2000) Anatomical location of bite marks and associated findings in 101 cases from the United States. Journal of Forensic Sciences, 45, 812-814.

Rahimi, M., Heng, N.C.K., Kieser, J.A. and Tompkins, G.R. (2005) Genotypic comparison of bacteria recovered from human bite marks and teeth using arbitrarily primed PCR. Journal of Applied Microbiology, 99, 1265-1270.

Rothwell, B.R. (1995) Bite marks in forensic dentistry: a review of legal, scientific issues. J Am Dent Assoc 126, 223–232.

U.S. Department of Justice, FBI (2010) Crime in the United States.

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