Gram Staining: Preparation Of Gram Staining

 GRAM STAINING

 Gram staining is common techniques consumed to distinguish two huge groups of bacteria depend on their various cell wall contents. The gram staining procedure differentiates among gram negative and gram-positive groups by colouring these cells violet or red. Gram positive bacteria stain violet because they having thick layer of peptidoglycan in their cell wall. Which contain the crystal violet these cells are stain with, instead of gram negative bacteria stain pink or red, which is characteristic of a thinner peptidoglycan cell wall. Which does not contain the crystal violet while the de colouring process.

 Gram staining involve in 3 process: staining with water soluble dye known as crystal violet. Decolorization, and counter

 (AppliChem product no. A0556) at 56°C. When the stain is dissolved completely take out the solution from heater. (Preferably, disperse at room temperature over night.

 Cover the container with parafilm in order to prevent contamination by moisture)

 Clear the solution by using whatman filter paper, after the solution became to room temperature

 Collect the filtered solution in a clean and dry brown glass bottle. Age the solution at least 2-3 days before using it first time.

 Store leishman’s stain solution at room temperature in tightly sealed bottles, protected from light and heat. Don’t store the solution near acid containing bottles.

 If stored correctly Leishmen’s stain solution is stable for approximately 3

You have to transfer him to the stem cell bank. By using your knowledge in regenerative stem cell theory, outline the stages of cellular differentiation which give rise to specialized cell types and identify a range of different cell types further to that relate the structure of specialized cell types to tissue function.

Everything in the body made up of cells (ex: hair, muscles, bones, fingers, organ even blood etc..) All these cells are consisting of same genetic material DNA. But these DNA material is totally different from each and every one else. That’s why it makes everyone unique individual characteristic. So that cells having ability to contain the same information.

Cellular