Escherichia coli (E. coli) is defined as a gram negative, rod-shaped, facultative anaerobic bacterium that consist of hundreds of different serotypes and is highly versatile. These serotypes range from strains that are harmless and play a vital role in maintaining intestinal function, to strains that contain pathogenic properties that infect the human body and cause distinct signs and symptoms. Pathogenic Escherichia coli strains are capable of causing three common clinical diseases that include
Escherichia coli (E. coli) is a member of the Enterobacteriaceae family of organisms. It is a non-spore forming, facultative anaerobic, gram negative rod capable of growing on a variety of media and, similar to other members of the Enterobacteriaceae family, contains the enterobacterial common antigen. Most E. coli are part of the normal flora of the human gastrointestinal tract, however some strains are pathogenic and capable of causing clinical disease. Epidemiologic classification of E. coli is based
Gentamycin, Neomycin, and Streptomycin on the Escherichia coli Bacteria Introduction: Escherichia coli, commonly known as E. coli, is a bacterium that is associated with food poisoning. Both in the medical community and the general public there are growing concerns about the health dangers that are associated with Escherichia coli. One major area of concern is its apparent resistance to certain core antibiotics. The bacterium Escherichia coli, is found in both foods and lakes. In terms of food it
E. Coli is a class of bacteria that is often found in the intestines of humans beings and animals. Most strains of E. Coli are harmless but they are few that can cause intense stomach pain, vomiting and diarrhea. In severe cases, it can lead to kidney failure. The Taco Bell E. Coli outbreak began in November 2006 in New Jersey. This outbreak sickened seventy-one individuals mainly in the NorthEast. Taco Bell originally blamed the E. Coli outbreak on the green onions they were currently serving; this
E. Coli Infection E. coli are bacteria that can cause an infection in various parts of your body, including your intestines. E. coli bacteria normally live in the intestines of people and animals. Most types of E. coli do not cause infections, but some produce a poison (toxin) that can cause diarrhea. Depending on the toxin, this can cause mild or severe diarrhea. This condition can spread from one person to another (contagious). Toxin-producing E. coli can also spread from animals to humans.
are achievable to reduce the risk of outbreaks. E. coli O157:H7 is frequently found on the hide, hoofs and in the digestive tracts of cattle. As a result of this, meat can potentially be contaminated during the slaughter and processing of the meat. Since E. coli O157:H7 contamination can cause serious human illness, it is critical to reduce this risk during slaughter and processing. Background • Escherichia coli, usually referred to as E. coli, is a large group of bacteria that is commonly found
Escherichia coli (E. coli) E. coli is a bacteria that normally lives inside the intestines of humans. There are many different strains some of which, cause human infections. E. coli infections are normally started through cross contamination with feces or stool of human or animals. E. coli is able to case Urinary Tract Infection because it resides around the anus. Since the anus and the urethra are so closely connected in women, this allows for direct transmission of E. coli. The virulence factor of E. coli
Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths, pH, and in the Presence of an Antibiotic Abstract The purpose in this experiment of growth dynamics of E. coli in varying media was to determine which media produces the maximum number of cells per unit time. First a control was established for E. coli in a 1.0x nutrient broth. This was used to compare the growth in the experimental media of 0.5x and 2.0x, nutrient broths; nutrient broths with an additional 5
E. Coli Growth in Succinate Versus Glucose Introduction All forms of life need a source of energy. This source of energy is carbon. (Manual.) Microscopic organisms are no exception to this rule. Organisms such as bacteria reproduce through binary fission, a process where the organism or cell grows and splits to produce two new daughter cells. In order for cells to grow and reproduce they must have an adequate supply of nutrients. Cells such as E. Coli can acquire nutrients through glycolysis or
2003, Lipps 2008 B). Our solution is to create a universal antivenom is modify a strain of Escherichia coli to produce LT-15 (Lipps & Lipps 2005). We will insert the LT-15 gene into a plasmid with a promoter, then transform E. coli with the plasmid and grow the recombinant strain. (Cawood 2013, Cohen et al. 1973, Huang et al. 2012, Lipps 2002 B, Lodish et al. 2000, Muyrersa et al. 2001). These E. coli can then be grown and harvested industrially for LT-15. This is a novel project because this would be
Escherichia coli from fecal material in newborns. Although it was initially thought to be a commensal organism, fifty years later scientists noticed that E. coli was in fact the cause of diarrhea in infants. E. coli is a gram-negative rod bacteria and a facultative anaerobe. This means that it can live with and without the presence of oxygen (1). Their preferred temperature in which they grow is 37 degrees. This is the equivalent to our body temperature, making our bodies very hospitable for E. coli growth
The purpose of the lab was to transform E.coli using the plasmid pRFP to promote the expression of antibiotic resistance as well as expression of the red fluorescent protein (RFP). The hypothesis was that if the transformation was successful, then the bacteria would express RFP because the arabinose would activate the plasmid’s red fluorescent protein, and show growth because pRFP allows E.coli to grow even in the presence of an antibiotic. The plasmid was combined with a sample of E.coli through
that are well known. However, anyone is susceptible to infection, healthy or not. I am healthy person physically and mentally. However, a healthy person can still be susceptible to infection. I was infected by the bacteria Escherichia coli, more commonly known as E. coli. While infected by a pathogen my body went through all of the periods of illness because the bacteria had made it through four out of five components that made it lethal. It led to a major kidney infection. There are five aspects
E. Coli 0157, written by Mary Heersink, is a nerve-racking, adrenaline-filled story of a mother's experience with a then unknown deadly bacteria. The book brings up many reactions in its readers, especially the questioning of the practice of doctors in hospitals. The reader's knowledge base of scientific procedures in emergency centers was widened as well as the knowledge of how to the human body reacts to different agents in its system. For Mary Heersink, all is good. And all that is not good can
The Ethics of Genetically Modified Food Production As human technological innovation proceeds into the twenty-first century, society is faced with many complex issues. Genetic engineering and cloning, encryption and information security, and advanced weapons technologies are all prominent examples of technological issues that have substantial moral and ethical implications. Genetic engineering in particular is currently a very volatile subject. One important aspect of this field is GMO or Genetically
Introduction Background Information and Research: Inserting a gene from the Aequorea victoria jelly fish into the DNA of rabbits, pigs, and mice genetically modifies them to glow-in-the-dark. The production of specific genes are coded by genes. This particular type of jelly fish naturally glows in the dark because a gene coded for a green fluorescent protein (GFP). The goal of genetically modifying organisms is to have the modified organism produce a protein that has been coded by the inserted gene
non-natural ingredients. Interestingly enough, a news report last week on TV indicated that organic produce, which is more expensive than regular produce, may actually be more dangerous to consume because it is fertilized with manure that contains E. Coli bacteria. There may be a consumer backlash against organic products if they are shown to pose a danger to the consumer. Complex technology, has also been a key marketplace feature of the fourth era of the consumer movement. Through the Internet, it
Ligation of EGFP into pET41a(+) vector transformed into E. coli cells followed by PCR amplification of extracted DNA plasmid for success evaluation along with gel electrophoresis at each step. Introduction Enhanced green fluorescent protein (EGFP) was originally isolated from a bioluminescent jellyfish called Aequorea victoria. As suggested by the name, this protein fluoresces green when exposed to light in the ultraviolet range. The ultimate goal of the following experiment was to successfully
being used in this test include: • 6 Mueller-Hinton agar plates • 14 cartridges of antimicrobial drugs • three automatic dispensers • two 1mL pipettes and pipette pump • broth culture of Staphylococcus aureus and Escherichia coli • spreading rod soaking in ethanol • two forceps soaking in ethanol • marking pen • ruler • antimicrobial sensitivity chart To start off this lab you will 1. Label the plates wit...
The most common infection involving Proteus mirabilis occurs when the bacteria moves to the urethra and urinary bladder. Although Proteus mirabilis mostly known to cause urinary tract infections, the majority of urinary tract infections are due to E. coli. One-hundred thousand cfus per milliliter in the urine are usually indicative of a urinary tract infection. Urinary tract infections caused by P. mirabilis occur usually in patients under long-term catherization. The bacteria have been found to move