Macromolecules In the Macromolecules lab we tested for different thing such as reducing sugar, starch, and protein. In the reducing starch experiment we tested three different macromolecules. Each macromolecules was tested for sugar and how we indicated how much sugar was in each macromolecules was because the color would change. Some of the colors it would change to would be light blue (no sugar), light green (a trace of sugar), yellow (low sugar) Orange (moderate), red orange (high). Seven tubes were tested that had 10 drops of a different liquid such as onion juice, potato juice, sucrose, glucose, distilled water, reducing sugar, and starch solution. In the Iodine starch test water, starch, milk, tofu, and apples was tested. The blue indicated that there was starch and yellow indicating there is no starch. In the biuret test for protein …show more content…
Set up tubes; labels 2. Add 1 mL of each sample to be tested. Make sure you stir the solution before pipetting it into your tube. 3. Add 2 mL of Benedict`s reagent to each tube 4. Place the tubes in a baker of boiling water for 3 minutes. 5. Remove the tube and allow them to cool 6. Examine each tube and record the solution color in the table 7. Rinse out the tubes; use the same labels for the iodine test Iodine Test for Starch 1. Set up tubes and label 2. Add 1 mL of each sample to be tested. Make sure you stir the solution before pipetting it into your tube 3. Add 7-9 drops of iodine (IKI) to each tube. DO NOT heat 4. Examine each tube and recorded the color in the table Procedure for the Biuret Test for Proteins 1. Set up tubes; label 2. Add 2 mL of 2.5% sodium Hydroxide (NaOH0 to each tube 3. Add 2 mL of each sample to be tested 4. Add 3-5 drops of Biuret reagent to each tube, mix well and let settle for 2 minutes. 5. Examine each tube and recorded the
this is the best volume to use as it is about ¾ of a test tube full,
We then took 1ml of the 10% glucose solution again using the glucose rinsed pipette and added it to test tube 1, we then filled the H2O rinsed pipette with 9ml of H2O and added it to test tube one; making 10ml of 1% solution.
The independent variable for this experiment is the enzyme concentration, and the range chosen is from 1% to 5% with the measurements of 1, 2, 4, and 5%. The dependant variable to be measured is the absorbance of the absorbance of the solution within a colorimeter, Equipments: Iodine solution: used to test for present of starch - Amylase solution - 1% starch solution - 1 pipette - 3 syringes - 8 test tubes – Stop clock - Water bath at 37oc - Distilled water- colorimeter Method: = == ==
When I dropped iodine to the leaf where I split off from the plant which had waited in red light, its color changed into blue. However, when I dropped iodine to the leaf where I split off from the plant which had waited in green light, its color stayed the same. It is because the plant waited in red light could make more photosynthesis than the other one, so it has more glucose and an iodine solution makes substance’s color blue if it has starch. It is used to test the presence of starch.
2. Put the test tube inside a beaker for support. Place the beaker on a balance pan. Set the readings on the balance to zero. Then measure 14.0g of KNO3 into the test tube.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The Benedict’s solution tested a very weak positive and showed a color of greenish-yellow. The weak positive means that the amylase enzymes were starting to get denatured because of the temperature change and lose their ability to produce simple maltose sugars. The enzyme is starting to lose its shape which affects its ability to perform the chemical reaction. So, the amylase was not able to fully break down all the starch into simple maltose sugars, only some of the starch. Since some of starch was broken down, there was some maltose present.
Put a label on each test tube. With a pencil, number each test tube from one to twelve.
The Benedict's Test is used to test the presence of simple sugars in a sample. If sugars are present, a color change will occur from blue to red. However, although the Benedict's test shows the presence of sugars, it cannot accurately determine the concentration of sugar in a sample solution. In our method, we added specific concentrations of glucose to the Benedict's test to use as a chart to estimate the glucose concentration of an unknown solution X. Although this gives a rough estimate of the concentration, it is very inaccurate. For example, the mystery solution X was a pale orange color, which was between the colors in my first and second test tube.
5.) One at a time, place your test tubes in the water bath and heat the first test tube to 25 , the second to 50 , the third to 75, and the last to 100 degrees c. Remeber to stir with your stirring rod every so often.
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =
* Size of potatoes * Diameter of each potato tube * Time in sugar solution We need to make sure in both experiments the fair test lists are used and the procedures are carried out. This needs to be done otherwise my results will not be accurate and will look odd. Method: Firstly we got out all our equipment.
2nd step heat the mixture: Make sure the agarose dissolves. Wait until it boils and when you are going to transfer the mixture, wear gloves to avoid getting burnt. Transfer the mixture into a removable gel tray.
Begin collecting samples with the pure hexane. Keep adding hexane so that the silica gel column does not run dry. Collect one 20 ml sample. Repeat with 90:10 hexane and collect 4 20-mL bottles. Repeat with 80:20 hexane and collect 2 20-mL samples.
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and