The Purpose of the Chemical Reaction Lab was to examine and measure the chemical reaction of H-C-L, hydrochloric acid and CuCl2, Copper chloride solution to determine different types of reactions, within specific elements and compounds; Aluminum foil, Al aluminum shot, Ammonium hydroxide, Magnesium, Silver nitrate, Sodium carbonate, Sodium bicarbonate, Sodium hydroxide, and Zinc. By completing the trials, the data displayed chemical changes. These changes were observable in changes of substances, such as formation of precipitate, change in color, release of gas, and difference of temperature.
The bacteria tested positive for being a facultative anaerobe, because the entire tube appeared cloudy. The bacteria grown everywhere in the test tube, meaning that my unknown bacteria can produce ATP in the presence of oxygen and it can also switch to fermentation in the absence of oxygen. A negative result would show a stub line after incubation, because the bacteria did not move. They can only move if they have flagella. A feather out or cloudy appearance of the test tube identifies a positive mobility result. After conducting the gram stain test, we still needed to confirm that the bacterium was truly a gram negative or gram-positive bacterium. The MacConkey media was used to grow gram-negative bacteria and inhibit the growth of gram- positive. The bile salts and crystalviolet ingredients are selective against gram-positive bacteria; it can also be used to differentiate between gram-negative bacteria. The MacConkey media allowed Providencia stuartii to grow in the test plate. I also did a lawn on the MSA plate, but the bacteria did not grow because MSA is selective against gram-negative. There is 5% salt in MSA limited the growth of gram- negative and Coliforms, such as E-coli. The second test that was done is the oxidase. This test was done to detect whether or not the organism is aerobic or not. To perform this test, I took a sterile
3. Drop tablet of Alka-Seltzer into the cold beaker of water and time length of reaction with a stopwatch
Experiment: First prepared a well plate with the appropriate amounts of distilled water, HCl, and Na2S2O3 in each well according to the lab manual. The well where the reaction
The ZnO crystallizes in a few forms which are wurtzite, zinc blende and rocksalt as shown in Figure 2.4. The wurtzite structure is the most thermodynamically stable phase under ambient temperature and also very common. The zinc blende ZnO geometry can be stabilized only by growth on cubic lattice substrates and the rocksalt structure (NaCl structure) can be obtained at relatively high pressures using ultrasonic wave velocity measurements of up to 10 GPa. (Morkoc).
This research includes information about Alka-Seltzer and it’s correlation with chemical reactions. Many people use the chemical reaction of Alka-Seltzers to heal themselves. Heat and cold can strongly affect the speed of a chemical reaction. This science project will determine the effect heat has on the reaction between a seltzer tablet and beakers of water. The temperatures will be extremely cold, cold, room temperature, hot, and boiling water.
The first step of the experiment that occurred is to take the two microcentrifuge tubes and label the first +pGLO and the second –pGLO. Next the micropipetter was set to 250 microliters and 250 microliters of calcium chloride was added to each microcentrifuge tube, using a fresh tip if it came into contact with the solution, then the tubes were put on ice until the bacterial was obtained. Next our group placed an E.coli colony into each of the tubes using a sterile loop and spinning the loop so that the bacteria was all in the solution, be sure to use a different sterile loop for each tube. Then we took a third clean sterile loop and placed it into the solution of the desired
In this experiment, we learned of effects and evidence of chemical change. We started the experiment with observance of a chemical reaction between the previously present 1.0 M of copper nitrate and the added 1.5 M of sodium hydroxide. When mixed with a stirring rod, this created a blue milky liquid known as sodium nitrate. The solution was then put into 50.0 ml of boiling water. This produced a reaction in the sodium nitrate, the reaction being a change from a blue milky liquid into a brown muddy liquid. The products of the chemical change being copper oxide and water. After a cooling process where there was no observed change, 1.5 M of hydrochloric acid was added to the present solution in the test tube. Once mixed with a clean stirring rod, the solution became transparent with the products of the change being copper chloride and
All equipment’s were collected along with lab coat and safety goggles. The lab bench was cleaned with disinfectant and paper towel. The Bunsen burner was switched on and the loop was flamed and used to smear bacteria from the petri dish. Once the slide was prepared. It was then passed through a Bunsen burner three times. Crystal violet was added to the slide and incubated for one minute. The slide was rinsed with a water for at least five seconds to remove crystal violet. Gram's iodine was poured into the slide for one minute as this secures the crystal violet to the bacterial cell wall. The slide was rinsed with an alcohol for three seconds and then washed with water. The alcohol used will decolorize the sample if it is Gram negative, it
The purpose for this procedure is for students to familiarize themselves with how different types of properties can lead up to identifying substances. For the safety of this experiment be sure to always have your goggles on and tightly secured, due to any splattering of chemicals or glass breakage. Under no circumstance should any chemical be near your mouth. In this procedure students will use four methods that will help identify what is their unknown substance. Once all methods are done its data will provide physical properties of numerical values which students will compare to the list that will lead to identifying their pure substance. The four methods in this experiment are finding the density (g/mL), the solubility, the melting point
The experiment was to investigate what are the products of a chemical reaction, more specifically, what iron compound is formed. A chemical reaction is anything that has had a color change, the formation of a solid, bubble, or a temperature change. In an oxidation-reduction reaction, charges of molecules are going to change. The first balanced equation was Cu〖SO〗_4+Fe(s)→Fe〖SO〗_4+Cu. The second balanced equation was 2Cu〖SO〗_4+3Fe(s) →3〖Fe〗_2 (〖SO〗_4 )_3+Cu. Given the two different chemical formulas, the theoretical yield was found to determine how much copper would be left over after the reaction by using the balanced chemical equations and stoichiometry. With the iron being the limiting reagent, we knew that the excess of copper product
The purpose of this investigation was to test the hypothesis that if a solution is more concentrated, then the reaction time of a chemical reaction including that solution would decrease because there would be less water molecules in the way of the two combining solutions. The results of this investigation do support the hypothesis.
The results of this experiment showed that 75% of the predicted outcome was correct, which left 25% to be incorrect. Based on the predicted outcomes, 3 out of the 4 (LB/amp/ara +, LB -, LB/amp +) petri dishes resulted as predicted. The results show growth in the LB/ara plate but, the prediction was for no bacterial growth to occur. Because of the ampicillin and arabinose mixup, this plate had bacterial growth. The antibiotics were not present in the agar to prevent the growth of bacteria. This error in the lab resulted in a somewhat of a flawed experiment, even though the majority of the plates turned out as expected.
Each group of bacteria was labeled with a different number. The objective was to find what three bacteria’s. Each student had obtained by doing different types of experiments. The first step was to streak the three methods in an aseptic technique. The two gram negative bacteria’s were to be T streak with one Helton Enteric, one streak of Mac Conkey, and one streak with Eosin methylene blue agar also known as EMB. The procedure was done just as it in the laboratory manual indicated. All the test tubes and plates were label and placed into the incubate to test for isolation for the next 48 hours. However, for the gram positive bacterium there was a streak with one Phenyl enthol Blood agar and also placed in the incubator for 48
In this lab the group studied the effect of temperature on rate of chemical reaction