Investigating How Quickly Amylase Breaks Down Starch With Varying Temperatures
Instructions List
I gathered the equipment shown below. I put 3 drops of iodine in each
chamber of the spotting tile. I boiled the kettle and mixed together
water from the kettle with water from the tap so the water reaches the
correct temperature. The water was put into a beaker. 5ml of starch
was put into a test tube. The test tube was placed into the beaker.
When the water in the beaker was at the required temperature the stop
clock was started. After one minute 1ml of amylase was put into the
test tube with the starch using a syringe or pipette. As soon as the
amylase and the starch had mixed a sample was taken from the test tube
using a pipette and mixed with the droplets of iodine in one of the
chambers of the spotting tile. The experiment was repeated many times
for different temperatures ranging from 20 degrees Celsius to 60
Celsius. The temperature goes up 5 degrees every time.
Fair Test
I will make sure that I measure the correct amounts of starch and
amylase each time. I will always put 3 drops of iodine in each chamber
of the spotting tile, no more, no less. I will clean out the test tube
and spotting tile thoroughly each time I repeat the experiment. I will
always measure the temperature of the water in the beaker accurately
with a thermometer.
Equipment List
Diagram
Ÿ A beaker
Ÿ A bottle of starch
Ÿ A bottle of amylase
Ÿ A bottle of iodine
Ÿ A test tube
Ÿ 2 spotting tiles
Ÿ Kettle
5. A second test tube was then filled with water and placed in a test
3.) Divide your 30g of white substance into the 4 test tubes evenly. You should put 7.5g into each test tube along with the water.
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How Amylase Concentration Affects the Rate of the Starch Concentration In this piece of coursework, I have to carry out an investigation to find out how amylase concentration affects the rate of starch. Enzymes are biological catalysts that speed up the chemical reaction that goes on inside living things. An enzyme acts on substrate and may do its job inside or outside the cells. However, the rate at which enzymes work are affected by the following factors/variables: Concentration:
I blanked it with 2 cm³ water, 1 cm³ amylase and 3 drops of iodine.
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the fact that the starch was left out so long. Being left out for a
being used up, for that is a property of a catalyst is, it speeds up a
and a fall in temperature will slow them down. In many cases a rise in
repeat the process 3 times in total to ensure a fair test. At the end
I prepared two large test tubes each should have an inch of KOH pellets on the
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it seemed to be closer to test tube 1 therefore I estimated it to be
== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method = == = =