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While the witches in the play provide the motivation for Duncan’s betrayal, they never instruct Macbeth to take his cousin’s life, yet Lady Macbeth does. Also, while Macbeth physically stabs his leader, it is only because of the instruction and manipulation of his wife. From as soon as she knows of the prophecies of the witches, Lady Macbeth is convinced that the king must die. Lady Macbeth is the most responsible for the death of King Duncan.