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List the importance of enzymes in living organisms
application of enzymes in medicine pdf
ROLES OF ENZYMES IN INDUSTRIES, IN ANALYTICAL BIOCHEMISTRY, IN MEDICINE AND
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Life Science ORT Papain Enzyme Mitchell Fieldgate Introduction: Enzymes in general are very interesting to learn from and are fundamental in carrying out processes in various organisms. Enzymes are proteins that control the speed of reactions, they help quicken the rate of the reaction and also help cells to communicate with each other. There are 3 main groups of enzymes, first are the metabolic enzymes that control breathing, thinking, talking, moving, and immunity. Next are the digestive enzymes that digest food and normally end with –ase, there are 22 known digestive enzymes and examples of these are Amylase, Protease, and Lipase. The final group are the Food or plant enzymes which is what my enzyme that I’m studying falls under. Papain gets its name because it comes from papaya fruit, its main purpose is to break down proteins and break peptide bonds however it is not only used in the Papaya fruit and has many external uses. It was also very helpful in the 1950s when scientists were trying to understand enzymes. It also helps us to this day understand Protein structural studies and peptide mapping. Without enzymes, reactions in the body would not happen fast enough and would tarnish our way of life which is why it is vital that we study and learn from them. Literature Review Enzymes as mentioned before help speed up reactions, they generally work by bonding to a substrate, this bonding occurs at the active site. This link then forms a different molecule which will benefit its respective process. Every enzyme has its own optimum pH level to work under, if too low the enzyme will be very slow. However if too high the enzyme will then denature and be obsolete. This is why it is important to know the optimum pH level for whatev... ... middle of paper ... ... still prevalent in enzyme research. The limitations to my research were that I only tested it on one type of protein which was gelatine even though the enzyme is more well known for its involvement in tenderizing meat. Another limitation is that I couldn’t test the strength of each in my experiment and could only check the consistency of each beaker. If I could test the strength of each beaker then my results would have been more conclusive and given me a better idea of how well the papain enzyme broke down the proteins. A recommendation would be that a more thorough experiment should be carried out in order to get a better picture of how well the papain enzyme can break down proteins. However my experiment did give me sufficient results as there was some evidence of the proteins in the gelatine getting broken down and therefore my research question was answered.
Catalase is a common enzyme that is produced in all living organisms. All living organisms are made up of cells and within the cells, enzymes function to increase the rate of chemical reactions. Enzymes function to create the same reactions using a lower amount of energy. The reactions of catalase play an important role to life, for example, it breaks down hydrogen peroxide into oxygen and water. Our group developed an experiment to test the rate of reaction of catalase in whole carrots and pinto beans with various concentrations of hydrogen peroxide. Almost all enzymes are proteins and proteins are made up of amino acids. The areas within an enzyme speed up the chemical reactions which are known as the active sites, and are also where the
Enzymes are biological catalysts, which are proteins that help speed up chemical reactions. Enzymes use reactants, known as the substrates, and are converted into products. Through this chemical reaction, the enzyme itself is not consumed and can be used over and over again for future chemical reactions, but with the same substrate and product formed. Enzymes usually only convert specific substrates into products. Substrates bind to the region of an enzyme called the active site to form the enzyme/substrate complex. Then this becomes the enzyme/products complex, and then the products leave the enzyme. The activity of enzymes can be altered based on a couple of factors. Factors include pH, temperature and others. These factors, if they become
Purpose: The purpose of this lab is to explore the different factors which effect enzyme activity and the rates of reaction, such as particle size and temperature.
In this experiment, in the first part, the best concentration of enzyme was determined by recording the absorption over time. In the second part, the best concentration was selected from the previous experiment which was C and the optimum pH was determined.
Pearson Baccalaureate: Standard Level Biology Developed Specifically for the IB Diploma describes enzymes as “protein molecules which act as catalysts for reactions. As catalysts, the real function of enzymes is to lower the activation energy of the reactions that they catalyze” (Ward, Tosto, McGonegal, & Damon, 2007). Enzymes are globular proteins that have an overall 3D structure. (George, 2014).
Enzymes help sustain life. They speed up chemical reactions by lowering the activation energy required to start a chemical reaction. For digestion, enzymes catalyze the chemical reaction and this helps break down the carbohydrates, protein and fats that are present. Enzymes operate better under certain conditions and operate not so well under some conditions. According to the background information read they usually work best with temperatures, substrate and product concentrations, salinity and pH. Different enzymes work different in
Enzymes are biological catalysts, chemical reactions. Enzyme may act are called substrates and molecules called enzyme converts these into different products. Enzyme are used commercially, for example, synthesis of antibiotics. The study of enzyme is called enzymology.
The efficiency of enzymes depends on a number of factors, such as temperature, pH and the concentration of the enzyme just to name a few. This experiment was conducted to show the effects of different pH levels on the activity of the catalase enzyme, the data obtained in this experiment supports the initial hypothesis of the experiment which states that the catalase enzyme will function optimally at a pH of 7 and efficiency will decrease as the pH of solutions moves further away from 7. This is true because in the experiment the test tube containing the distilled water which has a pH of 7, produced the highest amount of foam out of the four test tubes. The efficiency of the reaction can be determined by the amount of foam (foam = oxygen, which is the result of the breaking down of the hydrogen peroxide molecule into water and oxygen) produced, or the level of the fizzing that takes place when hydrogen peroxide is added. The pH of a solution that an enzyme has to function in is very important because if the pH turns out to be too low or too high the enzyme will denature making it unable to function, however, the pH of a solution may be far from the neutral level of 7 but the enzyme might not fully denature which means that the enzyme catalase would not lose its catalytic ability completely whereas a fully denatured enzyme’s shame will have been altered completely causing the active site and substrate to not fit together the way they’re supposed to. In certain reactions, the active site and enzyme substrate are still able to fit together which means that the reaction will continue to take place but at a very low rate, this seemed to be the case in one of the test tubes during the experiment. The foam produced in the react...
Enzymes are catalytic proteins that speed up biochemical reactions. Most enzymes are composed of proteins, while in some cases they are composed of RNA molecules called ribosomes. Enzymes are highly specific. Each enzyme catalyzes only one chemical reaction.
We need enzymes in order to survive, without enzymes some reactions would be too slow to keep you alive. Enzymes help cells communicate with each other to keep things under control in the cell. The purpose of this experiment is to understand the role of enzymes in maintaining life and to be able to identify and explain various factors that affect enzyme functions for example the
Enzymes are types of proteins that work as a substance to help speed up a chemical reaction (Madar & Windelspecht, 104). There are three factors that help enzyme activity increase in speed. The three factors that speed up the activity of enzymes are concentration, an increase in temperature, and a preferred pH environment. Whether or not the reaction continues to move forward is not up to the enzyme, instead the reaction is dependent on a reaction’s free energy. These enzymatic reactions have reactants referred to as substrates. Enzymes do much more than create substrates; enzymes actually work with the substrate in a reaction (Madar &Windelspecht, 106). For reactions in a cell it is important that a specific enzyme is present during the process. For example, lactase must be able to collaborate with lactose in order to break it down (Madar & Windelspecht, 105).
Enzymes are an important part of all metabolic reactions in the body. They are catalytic proteins, able to increase the rate of a reaction, without being consumed in the process of doing so (Campbell 96). This allows the enzyme to be used again in another reaction. Enzymes speed up reactions by lowering the activation energy, the energy needed to break the chemical bonds between reactants allowing them to combine with other substances and form products (Campbell 100). In this experiment the enzyme used was acid phosphates (ACP), and the substrate was p-nitrophenyl phosphate.
“Enzymes are proteins that have catalytic functions” [1], “that speed up or slow down reactions”[2], “indispensable to maintenance and activity of life”[1]. They are each very specific, and will only work when a particular substrate fits in their active site. An active site is “a region on the surface of an enzyme where the substrate binds, and where the reaction occurs”[2].
Enzymes are protein molecules that are made by organisms to catalyze reactions. Typically, enzymes speeds up the rate of the reaction within cells. Enzymes are primarily important to living organisms because it helps with metabolism and the digestive system. For example, enzymes can break larger molecules into smaller molecules to help the body absorb the smaller pieces faster. In addition, some enzyme molecules bind molecules together. However, the initial purpose of the enzyme is to speed up reactions for a certain reason because they are “highly selective catalysts” (Castro J. 2014). In other words, an enzyme is a catalyst, which is a substance that increases the rate of a reaction without undergoing changes. Moreover, enzymes work with
Purpose: This lab gives the idea about the enzyme. We will do two different experiments. Enzyme is a protein that made of strings of amino acids and it is helping to produce chemical reactions in the quickest way. In the first experiment, we are testing water, sucrose solution, salt solution, and hydrogen peroxide to see which can increase the bubbles. So we can understand that enzyme producing chemical reactions in the speed. In the second experiment, we are using temperature of room, boiling water, refrigerator, and freezer to see what will effect the enzyme.