Amelogenesis Imperfecta can be described as a disorder that causes a rare abnormal formation of the enamel (Swootleg, 2007). The Enamel component of teeth is generally comprised of mineral, which is regulated by various proteins within the enamel matrix. People who are diagnosed with Amelogenesis Imperfecta have dentition with abnormal color yellow, brown or grey. There are three main types of amelogeneis imprefecta. They are: Hypocalcified Amelogenesis, persons afflicted with this type of amelogenesis have hypomineralization of crystallites which can be caused by defective crystallite nucleation and subsequent mineralization; in hypoplastic amelogenesis imperfecta due to apposition. Hypomaturation amelogenesis imperfecta refers to defects in the crystallite formation during the maturing phase of development of enamel (C J. W., 1992). Hereditary and genetics has been known to be a common factor for the disease, which involves a number of allelic and non-allelic mutations. Molecular studies have identified that significant genetic heterogeneity within the X-linked forms have been determined (Lagerstrom, 1990).
Through other investigations it has shown that there are differences in the biochemical composition of enamel in the types of amelogenesis imperfecta. This includes the findings of abnormal protein content within the composition of enamel in Amelogenesis imperfecta teeth in comparison to normal enamel. (C. Witkop, 1976). The purpose of this experiment was to further investigate the enamel proteins in various types of amelogenesis imperfecta and to fully deduce if amelogenin was retained in the fully developed amelogenesis imperfect enamel. The primary Biochemical method used in this study was the SDS-Page Electrophoresis ...
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Digestion of the haemolytic and non-haemolytic cells allowed for easier identification of fragments during electrophoresis analysis. Lane 12 in figure 3 show the size markers of SPP1 digested with EcoR1 while lanes 6 and 7 show samples of pK184hlyA and pBluescript digested with EcoR1 and Pst1. Lane 4 was loaded with plasmid DNA from haemolytic cells digested with EcoR1 and Pst1 while lane 5 was loaded with EcoR1 and Pst1 digested DNA from non-haemolytic cells. There was a lack of technical success in both lanes due to no bands appearing in lane 4 and only a single band appearing in lane 5. Theoretically, two bands should appear in both lanes after successful to allow for fragment identification. A possible explanation for the single, large fragment in lane 5 is that successful digestion did not take place and the plasmid was only cut at one restriction site leaving a large linear fragment of plasmid DNA. The absence of bands in lane 4 could be because there was not enough plasmid loaded into the lane. Another possibility could be that low plasmid yield as obtained when eluting the experimental samples in order to purify it. Lanes 8 and 9 belonged to another group and show technical success as two bands were present in both the haemolytic (lane 8) and non-haemolytic (lane 9) lanes. If the
Jones, D. J., Munro, C. L., & Grap, M. J. (2011). Natural history of dental plaque accumulation
Hypophosphatasia is a rare genetic bone disorder characterized by osteoblast hyperactivity and bone remodeling with loss of, or incomplete, mineral deposition. It is comparable to osteomalacia and rickets, but maintains a unique set of characteristic identifiers (Mornet 2008; Brickley and Ives 2008). Also called, Rathbun’s Syndrome, hypophosphatasia can be autosomal dominant or autosomal recessive depending on the individual. Severe forms are usually transmitted as autosomal recessive with a recurrence risk of 25 percent, while milder forms can be transmitted as either autosomal dominant or autosomal recessive, with between 25-50 percent recurrence rates (Mornet 2008). Hypophosphatasia has an incidence rate of 1 in 100,000 live births (Brickley and Ives 2008; Wendling et al. 2001). In half of all cases, the condition is fatal.
Cagli NA, Hakki SS, Dursun R, et al. (Dec 2005). "Clinical, genetic, and biochemical findings in two siblings with Papillon-Lefèvre Syndrome". J. Periodontol. 76 (12): 2322–2329. doi:10.1902/jop.2005.76.12.2322. PMID 16332247
-Reilly Philip. Is It In Your Genes. Cold Spring Harbor Laboratory Press. 2004: 223-228. Print
(Binder, 386). Other collagen-containing extraskeletal tissues, such as the sclerae, the teeth, and the heart valves are also affected to a variable degree. OI has a "common feature of bony fragility associated with defective formation of collagen by osteoblasts and fibroblasts." (Smith, 1983, 13) This disease, involving defective development of the connective tissues, is usually the result of the autosomal dominant gene, but can also be the result of the autosomal recessive gene. Spontaneous mutations are common and the clinical presentation of the disease remains to be quite broad. (Binder, 386)
The article Poor Teeth was written by Sarah Smarsh with the goal in mind being to shed light on the issue between upper and lower class society in a particularly concrete way. Teeth and dental health are an easy thing for people to imagine in their head because everyone has a set whether they’re white and shiny or black and rotted. This makes it easy to draw a comparison between people that care for their teeth and those who don’t. However, access to dental knowledge and services which the lower class often times doesn’t have is very different between the poor and the rich. While the rich stroll through life showing off their perfect glossy white rows of teeth, there are less privileged people out there with barren mouths whose weak pale gums
Osteogenesis imperfecta is classified by type. In 1979, Dr. David Sillence developed this classification system. Sillence’s system is based upon method of inherit...
Osteogenesis imperfecta (OI), also known as brittle bone disease, is a rare genetic disorder with the main characteristic being that the bones break very easily, usually for no apparent reason. The major cause of osteogenesis imperfecta is a mutation in the genes that produce collagen. Collagen is the main protein that works toward the production of connective tissue. Individuals with this disorder will produce less collagen than needed, which causes the bone development to be endangered. This could result in bone deformities. There are four types of osteogenesis imperfecta, and in all four types you will see bone fragility with multiple fractures and bone deformities.
"The Species of the Secondary Protein Structure. Virtual Chembook - Elmhurst College. Retrieved July 25, 2008, from http://www.cd http://www.elmhurst.edu/chm/vchembook/566secprotein.html Silk Road Foundation. n.d. - n.d. - n.d.
Albuquerque, C., F. Morinha, J. Requicha, T. Martins, I. Dias, H. Guedes-Pinto, E. Bastos, and C. Viegas. "Canine Periodontitis: The Dog as an Important Model for Periodontal Studies." The Veterinary Journal 191.3 (2012): 299-305. University of Michigan Dearborn Library Catalog. Web. 18 Mar. 2014.
A 39 years old male adult attended for a regular 6 monthly dental check up and routine scaling. The patient reports to suffer from anxiety, he is a teacher, a non-smoker, non-drinker and a regular dental attender.
Dental plaque is broadly classified as supragingival or subgingival based on its position on the tooth surface toward the gingival margin. Supragingival plaque is found at or above the gingival margin. Therefore, it can be further differentiated into coronal plaque, whi...
If you have diabetes, you probably know that uncontrolled blood sugar levels can negatively affect various organs in your body, including the heart, kidneys, nerves and eyes; however, did you know that inconsistent blood glucose levels can also lead to periodontal disease? Periodontal disease often leads to dental pain which can make chewing difficult. There is also the possibility of tooth loss.
It is noticed that science develops in splashes, each significantly advancing our understanding of the nature. The 19th century was the time of rapid development of microbiology. In the search for secrets of nature science has reached extremely high and low energies as well as large and small length scales. Still until recently we knew very little about one of the most complex aspects of life – microbes and infection. Now we are at the dawn of efflorescence in molecular biology, which provides an excellent basis for application into several areas of biology like cancer and virology . Revelations of molecular biology become extremely crucial, as they provide insights into the way molecules work.