Cytoskeleton is a structure that help in maintaining cell shape, cell motion, cell division and transport of vesicles. There are three different protein filaments that make up the cytoskeleton, which are microtubules, intermediate filaments and actin filaments. In term of their functions, microtubules are the structure that support the cytoplasm as it is the major components of cilia and flagella. Contain alpha and beta tubulin, however, alpha tubulin is found in the trachea and esophagus. The trachea and esophagus have three major layers, inner layer (mucosa), middle layer (submucosa) and outer layer (muscularis). In this experiment, three groups prepared the slides, however each group did different treatments. Group one and two did antibody staining, group 1 treated their slide with the antibody of alpha tubulin, while group two treated their slide with BSA blocking solution. We are group three treated our slide with hematoxylin and eosin (nuclei stain not an antibody staining). The purpose of this experiment for our group was to see the fine structures of trachea and esophagus. The results of this experiment were as stated in figures 1-8, we could identify the trachea, esophagus and other tissues.
Introduction:
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Trachea is part of the respiratory system; its main role is to deliver gas to and from the bronchi and lungs. On the other hand, esophagus is part of the digestive system that functions in transferring the food from the mouth to the stomach. The trachea consists of pseudostratified epithelium tissues, while the esophagus consists of stratified squamous epithelium tissues. The antibodies that were used in this experiment produced as a protective action of the immune system that were stimulated by antigens. Proteins contain many antigenic determinates, which can be used to detect a specific protein subunit such as alpha
The immune system is made up of a network of cells, tissues, and organs that work together to protect the body, and it defends the body from “foreign invaders.” Immunity can be divided in two three different defenses, and these are defined as first, second and third lines of defense. The first line of defense for the immune system is the primary defense against pathogens entering the body from the surface in order to prevent the start of disease and infection. Some examples of the first line of defense is the skin, protecting the external boundaries of the body, and the mucous membranes, protecting the internal boundaries of the body. Although the skin and mucous membranes work on the internal and external boundaries, they both release chemicals
mucous layer of the organs in the digestive tract by means of enzymes and acids.
Obesity a risk factor in which excess body fat accumulates and can have negative effects on your health. Here we identify how the hormone insulin reacts in 3T3-L1 fibroblasts and its role on adipogenesis. Adipogenesis is the development of fat cells from pre adipocytes. Insulin is an important factor in the differentiation of 3T3-L1 pre adipocytes to mature adipocytes. Oil Red O (ORO) is used to demonstrate the presence of lipids in each different treatment. A spectrophotometer is used to get the optical density of liquid at the different insulin concentrations. One factor CREB is revealed from preadipocytes to mature adipocytes. By demonstrating how insulin triggers transcription factors. When cells are insulin induced CREB is activated in differentiation. Insulin increased the rate of differentiation and the amassing of triglycerides in 3T3-L1 cells . Insulin was able to induce adipogenesis by observing cell morphology and optical density of liquid from ORO stain. Insulin at 1 µg/ml had the optimal rate of differentiation compared to the other insulin concentrations. Morphology of cells changed significantly from Day 0 to Day 7 at 1 µg/ml and appeared larger and
Planaria are one of many free-living flat worms that can be found in marine, aquatic, and terrestrial environments. Certain characteristics of planaria worms include an acoelomate body, a gut with no anus, lack of a blood vascular system, and a simple nervous system. The main reason as to why planaria are subjected to many studies is because of their unique ability to regenerate. Regeneration is the ability to re-grow lost body parts that may have been cut off. This is possible because the organism has the ability to form a blastema, which is an accumulation of undifferentiated cells, at the site of the wound. Regeneration is capable of occurring at various degrees throughout the animal kingdom. This unique process would never be able to be seen in human beings. Humans and other mammals
Healthy lung tissue is predominately soft, elastic connective tissue, designed to slide easily over the thorax with each breath. The lungs are covered with visceral pleura which glide fluidly over the parietal pleura of the thoracic cavity thanks to the serous secretion of pleural fluid (Marieb, 2006, p. 430). During inhalation, the lungs expand with air, similar to filling a balloon. The pliable latex of the balloon allows it to expand, just as the pliability of lungs and their components allows for expansion. During exhalation, the volume of air decrease causing a deflation, similar to letting air out of the balloon. However, unlike a balloon, the paired lungs are not filled with empty spaces; the bronchi enter the lungs and subdivide progressively smaller into bronchioles, a network of conducting passageways leading to the alveoli (Marieb, 2006, p. 433). Alveoli are small air sacs in the respiratory zone. The respiratory zone also consists of bronchioles and alveolar ducts, and is responsible for the exchange of oxygen and carbon dioxide (Marieb, 2006, p. 433).
Unlike microtubules, actin filaments are globular chains made of a single monomer, which is called globular actin (G-Actin). Actins is somehow similar to tubulin in which actin subunits have binding site for a nucleotide, but it is mainly Adenosine Triphosphate (ATP), not GTP as in tubulins. Polymerization of actin filaments is also similar to microtubules polymerization in which assembly of subunit in head-to-tail orientation to create polarity. Actin filaments consist of two parallel helical protofilaments, or F-actin. In contrast with microtubules, actin filaments are the thinnest among cytoskeletal filaments, with a diameter of 5-9 nm, that’s why they are also known as ‘microfilaments’. Localization of actin filaments
Aim: The aim of this assignment will be to research the basic structure and function of Human Lungs and the respiratory condition known as asthma, and how its effects on the human lungs, looking at the causes and treatments used to prevent and treat the illness.
which make up the sweat glands in the skin and the mucus also lines the passageway inside the lungs,
All organism contain cells which need to produce energy. The way these cells produce energy is a process called cellular respiration. Cellular respiration is how these cells produce energy by turning glucose (C6H12O6) and oxygen (6 O2) into energy, water (H2O), and carbon dioxide (CO2 ). However, this leaves a large question, what factors affect cellular respiration? To test this we will use respirators to measure the lost oxygen of germinating beans and dry beans in room temperature water and cold water. The results we all expected to see was the germinating beans at room temperature to use the most oxygen. This was based on the idea that if a bean is germinating it would require more energy to grow and would
Acute asthma exacerbation occurs when individuals are exposed to an environmental trigger. The disease of chronic asthma creates a medium for hyperresponsiveness. The bronchial environment comprises of inflammation and inflammatory mediators. Consequently, when exposed to an antigen, “increased cellular permeability, smooth muscle contraction, and increased mucus production” occur (Huether & McCance, 2012, p. 689). The ...
Person, A. & Mintz, M., (2006), Anatomy and Physiology of the Respiratory Tract, Disorders of the Respiratory Tract, pp. 11-17, New Jersey: Human Press Inc.
The respiratory system has six major areas that works together that allows the body to breathe, prevent choking, and not to allow harmful debris to enter the respiratory system are some of the functions that the respiratory system does. The respiratory system is divided into two regions, the upper and lower respiratory. The upper respiratory consists of the Nose (nasal) and Pharynx while the lower respiratory embodies the rest of the system which includes the Larynx, Trachea, Bronchioles and Lungs. The information provided will be done by three individuals the upper respiratory tract will be provided by Mr. David Brown, the lower respiratory tract will be given by Ms. Brianna Agee and the infectious diseases will be provided by Mr. Derrek Woods.
In the lungs gas exchange occurs to re-oxidize the blood. Air travels through the respiratory tract to reach the lungs and back up to be exhaled into the environment. At the termination point of the respiratory tract lays the alveoli. The alveoli have a sac-like structure. In biological systems, the structure and functions of components are related. The alveoli have a structure specialized for efficient gaseous exchange. In the structure of the alveoli (alveolus), it looks as if it has the form of a hollow cavity that is paired with elastic fibers...
LAB REPORT 1st Experiment done in class Introduction: Agarose gel electrophoresis separates molecules by their size, shape, and charge. Biomolecules such as DNA, RNA and proteins, are some examples. Buffered samples such as glycerol and glucose are loaded into a gel. An electrical current is placed across the gel.
The Cell, the fundamental structural unit of all living organisms. Some cells are complete organisms, such as the unicellular bacteria and protozoa, others, such as nerve, liver, and muscle cells, are specialized components of multicellular organisms. In another words, without cells we wouldn’t be able to live or function correctly. There are Animal Cells and Plant Cells. In Biology class the other day we studied the Animal Cell. We were split into groups of our own and we each picked a different animal cell slide to observe. My group chose the slide,'; Smeared Frog Blood ';.