Analysis Of Gel Electrophoresis

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Introduction In the 1800s, forensic made some progress such as recording the first use of questioned document analysis, use of toxicology (arsenic detection) during jury trial, use of photography to identify criminals and finding evidences at the crime scene for documentation, and recorded its first use of fingerprints to help find potential witnesses or suspects for a crime. (Anonymous) In the 1900s, the first forensic science curricula were established by Swiss Professor R. A. Reiss in 1902, making it the first step towards adding forensic science as an academic class. Only till the 1930s did universities start offering class and degree for police science. During the 1950s, the University of California at Berkeley established one of the …show more content…

This causes the DNA fragments to move through the gel depending on their sizes. With this, the DNA fragments will show a sample that will determine how large they are to one another. Gel electrophoresis uses a horizontal gel-like slab. These gels are made of polysaccharide called agarose, which is dry, powdered flakes. When the agarose is heated in a buffer, it makes the gel form solid, slightly squishy gels. (Dickey, J. L. 2012) At one end of the gel, there is square shaped space that is called wells, this is where the DNA sample will be placed. Before the DNA samples are added, the gel must be added to the well of the gel box. One end of the box is hooked to a positive electrode and the other a negative electrode. When active, the DNA samples will start to migrate from the positive to the negative showing a difference with the DNA samples with some fragments that are larger and some that are smaller. (Mader. S. S. 2016) The experiment is to determine who is the suspect that caused the crime scene? There are two crime scenes that happened with two different suspects. They will be labelled as Crime Scene 1 and Crime Scene 2, while the two suspects will be labelled as Suspect 1 and Suspect 2. By using gel electrophoresis, this will determine who happened to be at the crime scene. First, is to put the DNA sample into the well of the gel box, make sure not to push it to deep or the DNA sample will be messed up. Next is to let it sit for a couple minutes till the gel is done moving. Then place the gel in lights to show the

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