Penicillin, ampicillin, and streptomycin disks were used to study E. coli antibiotic resistance. From these past studies, it is hypothesized that different antibiotics will result in varying zones of inhibition. If E. coli comes in contact with penicillin, ampicillin, and streptomycin, then the largest zone of inhibition will be from contact with ampicillin.
Materials and Methods
A tryptic soy agar plate was prepared. Four equal sections were drawn onto the bottom of the plate and numbered 1 to 4 in a counterclockwise formation. Then, a sterile swab was inserted into the bacterial culture and transferred...
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...eriment shows that different antibiotics exhibit different rates of effectiveness on bacteria. An ideal study would include a larger number of other antibiotics to experiment with in order to strengthen the prediction that smaller zones of inhibition will stem from bacteria with higher resistance to certain antibiotics. A future experiment could include testing the three antibiotics with a gram-positive bacterium, such as Staphylococcus aureus, to see if the difference in composition of the cell wall could affect its antibiotic resistance. One other study could involve being more specific and finding out exactly which parts of the bacterium are causing the antibiotic to not become effective. These future experiments may eventually result in the specific targeting of pathogenic bacteria, so that harmless bacteria that may actually assist the body will not be attacked.
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