Antibiotic Resistance Of E. Coli Essay

Antibiotic Resistance Of E. Coli Essay

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Antibiotics play a vital role in decreasing the death rate among humans with contagious diseases as they are chemicals produced by bacteria with the ability to control the growth of another bacteria. Yet, natural selection plays a major role in stimulating drug-resistant traits among bacteria. One of these bacteria is Escherichia coli, a normally harmless bacterium found in the digestive tracts of humans and animals. However, pathogenic variations of this bacterium may cause gastroenteritis, meningitis, and other extra intestinal infections (Tadesse et al., 2012). An increased use of antibiotics may cause the harmless E. coli to acquire this resistance and easily transfer it within the intestinal ecosystem, thus resulting in the decreased effectiveness (Olorunmola et al., 2013). E. coli can also have several antibiotic resistances, which makes it even more difficult to treat the resistant strains of pathogenic bacteria. It is important to study the increasing antibiotic resistance of E. coli because it is the most commonly recovered bacterial pathogen from hospital patients with urinary tract infections (Domínguez et al., 2002).
Penicillin, ampicillin, and streptomycin disks were used to study E. coli antibiotic resistance. From these past studies, it is hypothesized that different antibiotics will result in varying zones of inhibition. If E. coli comes in contact with penicillin, ampicillin, and streptomycin, then the largest zone of inhibition will be from contact with ampicillin.

Materials and Methods
A tryptic soy agar plate was prepared. Four equal sections were drawn onto the bottom of the plate and numbered 1 to 4 in a counterclockwise formation. Then, a sterile swab was inserted into the bacterial culture and transferred...


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...eriment shows that different antibiotics exhibit different rates of effectiveness on bacteria. An ideal study would include a larger number of other antibiotics to experiment with in order to strengthen the prediction that smaller zones of inhibition will stem from bacteria with higher resistance to certain antibiotics. A future experiment could include testing the three antibiotics with a gram-positive bacterium, such as Staphylococcus aureus, to see if the difference in composition of the cell wall could affect its antibiotic resistance. One other study could involve being more specific and finding out exactly which parts of the bacterium are causing the antibiotic to not become effective. These future experiments may eventually result in the specific targeting of pathogenic bacteria, so that harmless bacteria that may actually assist the body will not be attacked.

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