Many people use mothballs for different reasons. Mothballs are helpful for many different causes, but health risks are also associated with mothballs. Using mothballs can repel moths and make things smell great, but can also cause hemolytic anemia. Mothballs are helpful in many ways, but there is one factor in mothballs, Naphthalene, that can cause health issues. Mothballs have many features that are beneficial to people. One feature of mothballs is keeping a house or building free of pest. Mothballs
LAB 2: OSMOSIS, OSMOTIC PRESSUE, AND HEMOLYSIS Dierdra Renfroe Biology 340-002 Lab Partners: Ale Sanchez, Luke Brown, and Abby Fox September 15, 2016 INTRODUCTION Erythrocytes, or what are commonly known as red blood cells (RBC) within our bodies are constantly being faced with a changing environment. Tonicity is referred to as the concentration of solutes, permeable and nonpermeable, as well as the concentration of water both influencing the water that will come and goe through
processes, and to control both their intracellular and extracellular volume. In this cell volume experiment, one tested how different concentrations of sodium chloride (NaCl) solutions and osmolality affected the rate of absorbance and the percentage of hemolysis. When a solution has a higher NaCl concentration on the inside as compared to the outside, then it is a hypotonic solution. In this case, the red blood cells or erythrocytes can hemolyze (swell and burst). However, when the NaCl concentration is
Introduction The genus Listeria contains six species, two of which are considered pathogenic: Listeria monocytogenes and Listeria ivanovii. L. monocytogenes is the only species that is associated with listeriosis in humans (1). L. monocytogenes is a Gram-positive rod, facultative anaerobe; at room temperature, it exhibits tumbling motility due to peritrichous flagella (1). It has an optimal growth temperature of 32-35oC, but L. monocytogenes can grow between 0-45oC, pH 4.4-9.4, and a water activity
move into the cell. This solution was transparent after roughly 20 seconds, further validating that hemolysis had taken place. The isotonic and hypertonic solutions resulted in a turbid appearance, indicating no hemolysis (Meiselman et al.
Many steps were taken in order to narrow down and figure out which bacterium unknown 413 is. One of the first step taken in identifying unknown 413 was to do a gram stain. The gram stain, which was purple, resulted in gram-positive cocci. The gram-positive eliminated all the possibilities of unknown 413 being any gram-negative bacteria such as Escherichia coli, Enterococcus faecalis etc. The cocci morphology result eliminated any possibilities of it being any bacteria that are a rod. This left five
In lecture, we learned that hemolysis is what occurs when a blood cell comes into contact with a hypotonic solution. Also, that hemolysis is defined as the swelling and rupture of the blood cells. In lab, we tested how distilled water reacts with the blood cells; as we watched the cells, we could see them starting to swell and burst one by one. When we observed this, we knew right away that it was hemolysis, and that distilled water was hypertonic to the cells. When
Introduction: This report details the steps taken and processes used to discover the identity of the unknown organism given on Tuesday, November 28th, 2017. With all of the knowledge and skills gained over the semester, in class and in lab, unknown organism 6C was able to be positively identified. The objective of these labs was to successfully utilize the tests and procedures taught during the course to correctly identify the organism and to be able to explain the reasoning behind the tests used
clumped, and beaded cultures. 48 hour cultures of patients’ blood specimens with G. vaginalis were reported as mostly gram-positive (Catlin, 1992). G. vaginalis is beta-hemolytic on media containing human or rabbit blood but not on sheep blood agar. Hemolysis is improved by anaerobic incubation. Catlin (1992) reports that many different media have been used to isolate G. vaginalis from clinical specimens. The most successful media pos... ... middle of paper ... ...acteria and mycoplasmas. Additional
1. What are some of the other symptoms of TB disease not mentioned above? Other symptoms of TB disease are: fever, sweating, difficulty eating, fatigue, and weight loss. 2. In the U.S., Certain populations have a disproportionate rate of TB. Which populations are these? There are 4 states that have the highest rate of TB including, California, Texas, New York, and Florida. The races that have the highest rate of TB are respectively: Asia, Hispanic and African-American. Also, the rate of TB is
The hemolysis molar concentration for NaCl and glucose is 0.05 M. Adding red blood cells to an isotonic solution, there will be no isotonic pressure and no net movement. The isotonic solution leaves the red blood cells intact. RBC contain hemoglobin which absorbs light, hemoglobin falls to the bottom of the tube and no light is absorbed. Determining the isotonic concentration of NaCl and glucose by finding the lowest molar concentration. In contrast to isotonic molar concentration, hemolysis can
Microbiological characteristics Streptococcus pneumoniae is a gram-positive coccus. Usually they are found in pairs of cocci, or diplococci, but they may also occur in short chains or singly. When cultured on blood agar they demonstrate alpha hemolysis. They are non motile organisms. Virulence Virulence is caused by the chemical composition of the capsule. There are over 90 serotypes of S. pneumoniae which causes great difficulty when trying to develop a vaccine for this bacterium. The capsule
In order to understand the plasmolysis and hemolysis experiments we must first know what osmosis is. Osmosis is the movement of water from particles through the plasma membrane from an area of higher concentration to an area of low concentration. Both animal and plant cells undergo osmosis. The results of the cells vary due to the movement of water from high to low concentrations. Hypotonic, isotonic and hypertonic solutions all results with in the cell. In the animal blood cell experiment, the cell
“The purpose of the Act is to provide a consistent legislative framework for issues relating to whole body donation and the taking, storage and use of human organs and tissue.” (“Human tissue act - explanatory notes,” 2004) Back Ground The human tissue act, first was idealized in 1999 to 2000, based on the events at Bristol Royal Infirmary and the Royal Liverpool Children’s Hospital (Alder Hey). In this case organs and tissues were removed from deceased children without proper consent from the parents
Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November 2000) The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated
Results Observing under 1000X magnification, the gram stain depicts purple bacilli, as seen in Figure 1. Figure 2 depicts growth of a light brown, opaque bacteria on a Phenylethyl Alcohol Agar (PEA) plate. As seen in Figure 3, the blood agar plate shows brown growth surrounded by a very thin clear area. Figure 4 shows an endospore stain of the gram-positive unknown. It depicts green endospores surrounded by pink bacilli. Figure 5 depicts a slant of gram-positive isolate, which appears as an opaque
Erythrocytes are naturally a biconcave disc, which results in a larger membrane surface to volume ratio than a sphere shaped disc. These cells have the strength and flexibility needed to survive for 120 days in circulation. Their peripheral proteins stabilize the membrane and are responsible for their shape. These proteins include sprectrin, actin, ankryn, and band-4-protein. Peripheral proteins are attached to the red blood cell (RBC) membrane. Ankyrin-1 stabilizes the membrane by linking beta spectrin
INTRODUCTION The purpose of the microbiological experiment was to applying different types of techniques and methods learned in microbiology lab to a bacteria so that it may be accurately and precisely identified. “The words accurately and precisely are not the same in microbiology, accurately has the meaning of if repeated the same answer continues to be the result, precisely has the meaning of the correct answer.” “Microbiological experimentation often involves test that determine the ability of
Monitoring the TAT is one of the most important part of laboratory services. It is considered as the key performer indicator and many clinicians used it to judge the quality of a laboratory. In many critical care laboratories unsatisfactory TAT is a major source of complaints and it directly delays the patient treatment process. To improve the TAT in critical care laboratories, implementing the appropriate corrective measures are always important to maintain the quality assurance and provide the
discs. Over time when passing through the spleen pieces of the membrane are removed, causing the red blood cells to become round in shape, hence the term Spherocytosis (Seattle Childrens). When red blood cells enter the spleen the cells undergo hemolysis. Hemolysis in hereditary spherocytosis results in the interplay of an intact spleen and an intrinsic membrane protein defect (Medscape). The breakdown of red blood cells is called hemolytic anemia (Wint Carmella). A normal red blood cell can live up to