INTRODUCTION The purpose of the microbiological experiment was to applying different types of techniques and methods learned in microbiology lab to a bacteria so that it may be accurately and precisely identified. “The words accurately and precisely are not the same in microbiology, accurately has the meaning of if repeated the same answer continues to be the result, precisely has the meaning of the correct answer.” “Microbiological experimentation often involves test that determine the ability of an organism to use or produce some chemical, or to determine the presents or absence of a specific organism in a sample.” The scientific methods that were performed aided in the precise identity of the bacteria. Since September we have studied different types of bacteria in lab on Tuesdays and Thursdays, along with the readings in the Laboratory manual. Many of these organism we have come into contact with a one time or another and continue to do so. Identification of these bacteria is necessary because everything we do such as eating, drinking, cleaning, and healthcare, just to name a few, effects all living things ???? The …show more content…
Proteus vulgaris is a rod-shaped Gram-negative chemoheterotroph bacterium. The size of individual cells varies from 0.4~0.6μm by 1.2~2.5μm. P. vulgaris possesses peritrichous flagella, making it actively motile. It inhabits the soil, polluted water, raw meat, gastrointestinal tracts of animals, and dust. The invader P. vulgaris has numerous factors including fimbriae, flagella, outer membrane proteins, lipopolysaccharide, capsule antigen, urease, immunoglobulin A proteases, hemolysins, amino acid deaminases, and, finally, the most characteristic attribute of Proteus, swarming growth, enabling them to colonize and survive in higher organisms (Rozalski et al.1997 and Emody et
The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated at room temperature, the second at 30 degrees Celsius, and the third at 37 degrees Celsius. By placing each plate at a different temperature optimal growth temperature can be predicted for both species of bacteria.
In this lab project, the microbiology students were given 2 unknown bacteria in a mixed broth each broth being numbered. The goal of this project is to determine the species of bacteria in the broth. They had to separate and isolate the bacteria from the mixed broth and ran numerous tests to identify the unknown bacteria. The significance of identifying an unknown bacteria is in a clinical setting. Determining the exact bacteria in order to prescribe the right treatment for the patient. This project is significant for a microbiology students because it gives necessary skills to them for future careers relating to clinical and research work.
They can be found anywhere and identifying them becomes crucial to understanding their characteristics and their effects on other living things, especially humans. Biochemical testing helps us identify the microorganism present with great accuracy. The tests used in this experiment are rudimentary but are fundamental starting points for tests used in medical labs and helps students attain a better understanding of how tests are conducted in a real lab setting. The first step in this process is to use gram-staining technique to narrow down the unknown bacteria into one of the two big domains; gram-negative and gram-positive. Once the gram type is identified, biochemical tests are conducted to narrow down the specific bacterial species.
The purpose of this laboratory is to learn about cultural, morphological, and biochemical characteristics that are used in identifying bacterial isolates. Besides identifying the unknown culture, students also gain an understanding of the process of identification and the techniques and theory behind the process. Experiments such as gram stain, negative stain, endospore and other important tests in identifying unknown bacteria are performed. Various chemical tests were done and the results were carefully determined to identify the unknown bacteria. First session of lab started of by the selection of an unknown bacterium then inoculations of 2 tryptic soy gar (TSA) slants, 1 nutrient broth (TSB), 1 nutrient gelatin deep, 1 motility
Jennifer Ackerman's main focus in her article The Ultimate Social Network, is that of the functions concerning bacteria within humans. Although scientists have had presumptions about humans being proficient in governing their body’s innermost structure, they soon come to recognize the sophistication of our inner space which holds an extensive plethora of bacteria and other microorganisms that lie within each and every one of us. Moreover, scientists' new and emerging view of how the human body operates, and the cause of increasing present-day diseases (i.e. obesity and different autoimmune disorders) are uncovered by analyzing effects of certain microbe species in our bodies. By italicizing on points such as the above, in conjunction with bacteria's genetic variations, and modern computing technology, the author proves that scientists are quickly progressing with the characterization the most prevalent species of microbes, which, in her opinion, is definitely paying off.
Proteus mirabilis is part of the Enterobacteriaceae family. It is a small gram-negative bacillus and a facultative anaerobe. Proteus mirabilis is characterized by its swarming motility, its ability to ferment maltose, and its inability to ferment lactose. P. mirabilis has the ability to elongate itself and secrete a polysaccharide when in contact with solid surfaces, making it extremely motile on items such as medical equipment.
Bacteria play a large role in our health, the environment, and most aspects of life. They can be used in beneficial ways, such as decomposing wastes, enhancing fertilizer for crops, and breaking down of substances that our bodies cannot. However, many bacteria can also be very harmful by causing disease. Understanding how to identify bacteria has numerous applications and is incredibly important for anyone planning to enter the medical field or begin a career in research. Having the background knowledge of identifying an unknown bacteria may one day aid healthcare professionals diagnose their patient with a particular bacterial infection or help researchers determine various clinical, agricultural, and numerous other uses for bacteria.
I identified the genus and species of an unknown bacterial culture, #16, and I applied the following knowledge of morphologic, cultural and metabolic characteristics of the unknown microorganism according to the laboratory manual as well as my class notes and power point print outs. I was given an incubated agar slant labeled #16 and a rack of different tests to either examine or perform myself; the tests are as follows: Gram Stain; Nutrient Gelatin Test; Carbohydrate Fermentation; Dextrose, Lactose and Sucrose; IMVIC tests; Citrate, Indole, Mythel-Red and Vogues Proskauer test; as well as a Urease and TSI Test. Materials and Methods/Results Upon receiving the Microorganism (M.O.) #16, I prepared a slide by cleaning and drying it. Then, using a bottle of water I placed a sterile drop of water on the slide and used an inoculating loop, flame sterilized, I took a small sample of the unknown growth in my agar slant and smeared it onto the slide in a dime sized circle and then heat fixed it for ten minutes.
An understanding of the scientific method is important in the microbiology laboratory. The scientific method is known in the microbiology world to be the steps that are followed by scientists to answer specific questions about the world. Scientists who work in laboratories especially a microbiology laboratory use the scientific method. The purpose of the scientific method is to construct an accurate, reliable, consistent representation of the world. The scientific method involves steps such as asking a question, doing background research, constructing a hypothesis, testing your hypothesis by doing an experiment, analyzing your data and drawing a conclusion, and reporting your results. Using the scientific method is to clearly do an experiment
...standing the nature of relationship between the residing microbes inside human cells and about their function is very important to put an end to this war and to live in peace with the natural organisms that are benefitting human body and their survival has become our primary importance.
This may sound like the beginning of a horror movie, but it isn’t. It is actually a very basic description of a very broad subject: microbiology.
If certain types of bad bacteria were growing in your home and scientists knew how to keep that bacteria from spreading quickly, it could help people with serious health conditions. B. Background Information When you hear the word bacteria, the first thing that might come to mind is a germ. Although there can be many harmful types of bacteria, it is an essential part of life. Bacterium is all over the world and can be found in anything. Bacterium is one of the earliest life forms on Earth, and it plays a big role in how the Earth was developed.
Microbes are major key components in both are homes and industrial food preparation. There are number of lactic acid which is a form of bacteria which is a large group of beneficial bacteria used in certain foods while they are getting prepared such as yogurt, cheese, sour cream, butter milk and other type of fermented milk products. Things such as vinegars are produced by bacterial acetic acid fermentation. Yeast is also major use in the making of beer and wine and also for the leaving of breads. This also involves fermentations to convert corn and other vegetable carbohydrates to also make beer, wine or gasohol but also bacteria is the agents of are other foods. Other fermented foods will include things such as soy sauce, olives and cocoa. (Microbes and human life, 2013) Single cell proteins are known as dried cells of microbes which are used in protein supplement shacks. They are also called “novel food” and “minifood”. The production of this requires micro-organisms which then serve as the protein source and then the substrate which is biomass which they grow on them. There are a number of both these sources that we are able to use for the production of single cell protein (SCP). The micro-organisms used belong to the following groups of Algae, Fungi and bacteria. (Slide Share, 2012)
Knowledge is power when discussing the classes you have taken in college and how it affects your everyday life. Microbiology is one of those important classes where although for a majority of majors you do not have to take it and therefore will not learn the information discussed in it, other majors such as biology and nursing require you to take it. They will require you to take it because you will encounter some of the information being taught in it in the work field. As a nurse practitioner I have worked in the field with many patients who were diagnosed with Leprosy, Escherichia coli, Salmonella and other bacterial diseases. All of these diseases that I have encountered in patients were ones I was familiar with due to learning about them as a student in microbiology at CSUB. My children plan on