In this experiment, we determined the isotonic and hemolytic molar concentrations of non-penetrating moles for sheep red blood cells and measured the absorbance levels from each concentration. The results concluded that as the concentration increased the absorbance reading increased as well. A higher absorbance signifies higher amounts of intact RBCs. The isotonic molar concentration for NaCl and glucose is 0.3 M. The hemolysis molar concentration for NaCl and glucose is 0.05 M. Adding red blood cells to an isotonic solution, there will be no isotonic pressure and no net movement. The isotonic solution leaves the red blood cells intact. RBC contain hemoglobin which absorbs light, hemoglobin falls to the bottom of the tube and no light is absorbed. Determining the isotonic concentration of NaCl and glucose by finding the lowest molar concentration. In contrast to isotonic molar concentration, hemolysis can be determined by finding the …show more content…
However not in sucrose, the RBCs were semi-permeable. RBCs diffuse in the water around five minutes, but in glycerol RBCs diffuse in fifteen minutes. Several factors are involved that affect the rate at which the RBCs diffuse, could have been because of the size, polarity, or the charge of the molecule. Urea is the carbonic acid found in urine, blood, and lymph; it is formed in the liver from amino acids and ammonia. It is important that urea is permeable because the amount of urea in the body is essential because it helps undergo waste product. Glycerol is combination of sugar and alcohol. This solution is an important component for storage of fats that are ingested into the body as food, this one good reason why glycerol is permeable. Sucrose however has low permeability which is why sucrose has a slow rate of diffusion and glycerol and urea on the other hand has fast rates of
Data from Table 1. confirms the theory that as the concentration of glucose increases so will the absorbance of the solution when examined with the glucose oxidase/horseradish peroxidase assay. Glucose within the context of this assay is determined by the amount of ferricyanide, determined by absornace, which is produced in a one to one ratio.1 Furthermore when examining the glucose standards, a linear calibration curve was able to be produced (shown as Figure 1). Noted the R2 value of the y = 1.808x - 0.0125 trend line is 0.9958, which is statistically considered linear. From this calibration curve the absorbance values of unknowns samples can be compared, and the correlated glucose concentration can then be approximated.
In Blood In Blood Out is a drama directed by Taylor Hackford, and starring Damian Chapa (Miklo), Benjamin Bratt (Paco), and Jesse Borrego (Cruz), produced by Hollywood Pictures. The film was based off everyday life in East Los Angeles, from the 1970’s through the 1980’s. Damian Chapa stars as Miklo in the film, a Mexican-American who wanted to be accepted, not by his skin but for the Mexican within him. Benjamin Bratt (Paco) was the older cousin of Miklo, who learned his lesson throughout the movie and changed his ways. Jesse Borrego (Cruz) is the step-brother of Paco who was a talent artist, who ended up turning to drugs because of back problems caused by a rival gang incident.
Haemolytic colonies were classified by a white ring around the patched colony, indicating that haemolysis of the blood agar occurred. Conversely, non-haemolytic colonies were classified by a lack of a white ring, which indicated that no haemolysis took place.
Three hundred and thirty-four years later in the future, Carl Landsteiner, a Viennese doctor, performed a very simple experiment with blood in 1901. During his experiment, Landsteiner noticed "clotting in some samples of mixed blood and not others". (Tucker, 10) Landsteiner separated his samples into three groups: A, B, and C, according to how they clotted in his experiment. Today, the blood type C is known as type O blood. When Landsteiner was grouping these blood types, he happened to look over type AB. AB occurs in about 3 percent of the population. Later in 1907, two researchers, Jan Jansky in Czechoslovakia and William Lorenzo Moss in the United
Thyroid and metabolism hormones play a large role in the daily lives of all living species. Thyroid hormones regulate the metabolism and the metabolism is responsible for maintaining a specific range for the biochemical reactions that occur in the body (Martini 2014). The most important hormone for metabolic maintenance is thyroxine (T4). This hormone also plays a large role in body heat regulation. It is produced by the pituitary gland and secreted by the thyroid gland. The thyroid releasing hormone (TRH) must trigger the thyroid stimulating hormones (TSH) to release thyroid hormones to the thyroid gland. These hormones are under control of the hypothalamus, or main neural control center. Propylthiouracil (PTU) is a medication used to treat
In William Golding’s Lord of the Flies, blood gives the boys feelings of power and control through violence. For instance, after the hunters successfully kill a pig, Jack “giggled and flicked them [his hands] while the boys laughed at his reeking palms. Then Jack grabbed Maurice and rubbed the stuff [blood] over his cheeks” (135). Maurice is not a leader on the island and he is entirely comfortable with being a follower and not making decisions for himself. Jack, on the other hand, has more power in island politics and Maurice could arguably be his subordinate; therefore, the smearing of the blood over Maurice’s face is Jack demonstrating his control over him. Another example of the use of blood as a symbol of control is when a lightning storm
The slight differences in the way their atoms are arranged give them slightly different properties. These are shown below: α-glucose: β-glucose: Galatose: Fructose: [IMAGE] [IMAGE] The main function of monosaccharide is that they are able to move through bodies, gut walls and therefore important as a source of energy. All other carbohydrates have to be converted to monosaccharides before energy can be released and its is due to it’s small size they are very soluble and it is the form of monosaccharides that all carbohydrates are carried in the blood.
Wallace Terry has collected a wide range of stories told by twenty black Vietnam veterans. The stories are varied based on each experience; from the horrific to the heart breaking and to the glorified image of Vietnam depicted by Hollywood. Wallace Terry does not insinuate his opinion into any of the stories so that the audience can feel as if they are having a conversation with the Vietnam Veteran himself. Terry introduces the purpose of the book by stating, “ Among the 20 men who portray their war and postwar experiences in this book. I sought a representative cross section of the black combat force.”(p. XV) Although the stories in this book were not told in any specific order, many themes became prominent throughout the novel such as religion, social, and health.
In the article, Blood Red Night, author Lauren Tarshis' point of view is focused mostly on the destruction that the fire caused. I know this because most of the ideas that she conveys in the text use words that have to do with destruction. For example, in the section "A Choking Fog", the author states, "The blazes burned hundreds of acres of forestland and incinerated hundreds of homes and shops in nearby communities." This sentence uses several words such as blazes and incinerated which illustrate for the reader the destruction that happened during the peshtigo fire. Another example of when the author mentions the destruction that the Peshtigo fire caused was in the section "Sheets of Flame" when she states, "By morning, more than a billion
...s a component monomer of starch. As a monomer as opposed to a polymer, it is much smaller and would thus be able to cross the plasma membrane. However, glucose is a larger solute than the component ions of salt, thus meaning that simple diffusion would not be sufficient. Instead, facilitated diffusion would be needed to transport the glucose. However, in the dialysis tubing, there is no facilitated transport like there is for the plasma membrane. Thus, the glucose may pass through the dialysis tubing, but it would not be due to transport, but the artificial enlargement of the passages in the dialysis tubing. Water would move freely inside and outside of the cell, however, because there is a greater solute concentration inside the cell, the water would diffuse through osmosis into the cell model, increasing the final mass of the dialysis tubing and causing cytolysis.
Hemoglobin is liable to participating in harmful reactions with other oxidized substances and thus creates unstable products. In this experiment they were trying to see if they can reduce the number of free radicals being created during certain steps of the process to produce blood substitutes. They measured the amount of oxy hemoglobin then measured how much reacted with the nitrate depending on what other substance it was paired with to test their hypothesis. The amount of the oxy hemoglobin as well as the nitrate influences the reaction time and how
The Cell, the fundamental structural unit of all living organisms. Some cells are complete organisms, such as the unicellular bacteria and protozoa, others, such as nerve, liver, and muscle cells, are specialized components of multicellular organisms. In another words, without cells we wouldn’t be able to live or function correctly. There are Animal Cells and Plant Cells. In Biology class the other day we studied the Animal Cell. We were split into groups of our own and we each picked a different animal cell slide to observe. My group chose the slide,'; Smeared Frog Blood ';.
The overall purpose of the experiments in this lab was to introduce the function and structure of the plasma membrane, describe the workings of diffusion and osmosis, and to demonstrate how different factors such as particle size, temperature, and space of diffusion area affect the rate of diffusion. With the results from the experiments, it can be concluded that all of the factors listed do affect diffusion, and there may be many more than are unaccounted for.
Red Blood Cells contain hemoglobin molecules to help bind to oxygen to bring to other tissues. Without this function, cells would not be able to go through the process of cellular respiration and can only survive a short time. Red Blood Cells are also able to carry bicarbonate as a waste product and carry a variety of hormones to communicate between organs.
fluid for you. You can check the count on a hemacytometer if you want, but no one does