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Sample lab report biology macromolecules
Sample lab report biology macromolecules
Sample lab report biology macromolecules
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Benedict, Biuret, Iodine and brown paper bag tests were conducted in order to identify various macromolecules which might be present in the two unknown substances given. Qualitative data was gathered on the bases of clarity, viscosity, odor, state of matter and most importantly color change. The data gathered was then complied in table two of this lab. Table 2 represents the data compiled for unknown substances #6 and #7. When substance #6 was tested for the presence of protein, it did change color from blue to yellow, however this color change indicated a negative result. According to the results gathered in Table one, Part one of this lab, a color change from blue to light purple indicates a positive result; not blue to yellow. When Iodine
The purpose of the Unknown White Compound Lab was to identify the unknown compound by performing several experiments. Conducting a solubility test, flame test, pH paper test, ion test, pH probe test, conductivity probe test, and synthesizing the compound will accurately identified the unknown compound. In order to narrow down the possible compounds, the solubility test was used to determine that the compound was soluble in water. Next, the flame test was used to compare the unknown compound to other known compounds such as potassium chloride, sodium chloride, and calcium carbonate. The flame test concluded that the cation in the unknown compound was potassium. Following, pH paper was used to determine the compound to be neutral and slightly
Introduction: Someone in a restaurant has suddenly fallen ill and a mystery powder has been discovered with the victim. As the chief investigator, your duty is to identify the mystery substance through a lab. In this lab, it will consist of five known compounds and one unknown compound. Your job is to distinguish which one out of the five substances is the mystery powder. To figure out the mystery matter you will have to compare their physical and chemical properties and match them with the appropriate compound. Furthermore, you will compare their reactions with water, universal indicator, vinegar and iodine solution to discover what the mystery powder is.
To uncover organic compounds like carbohydrates, lipids, proteins and nucleic acid, by using tests like Benedict, Lugol, Biuret and Beta Carotene. Each test was used to determine the presents of different organic molecules in substances. The substances that were tested for in each unknown sample were sugars, starches, fats, and oils. Moreover, carbohydrates are divided into two categories, simple and complex sugars. Additionally, for nonreducing sugars, according to Stanley R. Benedict, the bond is broken only by high heat to make make the molecules have a free aldehydes (Benedict). As for Lipids, there are two categories saturated and unsaturated fats. One of the difference is that saturated fats are mostly solids and have no double bond (Campbell Biology 73). The Beta Carotene test works by dissolving in a lipid, thus giving it color to make it visible. Moreover, proteins are made out of amino acids that are linked by a polypeptide bond (Campbell Biology 75). The purpose of this experiment was to determine whether an unknown class sample or food sample had any carbohydrates, lipids, or proteins in it. The expected result of the lab was that some substances would be present while other would be absent.
Before being placed in the ice bath, the product appeared to have a golden tint to it and was fairly fluid. After the ice bath, the product remained fairly fluid, however, it appeared to take on a more cohesive droplet form and the color was less pigmented. In addition to this, the bromine test performed at the end of the investigation yielded slightly varying results. The color change identified in the case of the bromine/dichloromethane and added 5 drops of olive oil turned out to change the original orange/red color of the bromine/dichloromethane to a clear and unpigmented liquid. The addition of hydrogenated olive oil (product) to the originally orange/red colored bromine/dichloromethane ended up turning clear similar to the addition of olive oil, however, there appeared to be a slightly yellow tint to the resulting liquid as
Experiment #3: The purpose of this experiment to test the chromatography of plant pigments the alcohol test strip test will be used.
Secondly, the protein test was held by using the Biuret Reagent to indicate whether the substance contains proteins. The positive test result will show the color change to pink or purple. When Biuret was added into the substance, its colour
This line graph shows how some phenotypes were more successful than others. This is an accurate representation of natural selection. The dark blue, pink, and orange phenotypes became extinct before the experiment was even finished. The successful phenotypes were green, purple, and yellow.
When I dropped iodine to the leaf where I split off from the plant which had waited in red light, its color changed into blue. However, when I dropped iodine to the leaf where I split off from the plant which had waited in green light, its color stayed the same. It is because the plant waited in red light could make more photosynthesis than the other one, so it has more glucose and an iodine solution makes substance’s color blue if it has starch. It is used to test the presence of starch.
The hypothesis stated that when the spinach extract was placed on the polar paper chromatography the pigment would separate into different pigments on the...
= Before conducting the experiment I would conduct a simple test for the protein by placing a sample of the albumen into a test tube and add biurett reagent. This contains copper (II) sulphate and sodium hydroxide.
Macromolecules are large molecules that are made up of many smaller molecules and can be found in living things. They are formed through polymerization. Polymerization occurs when smaller molecules (monomers) join together to create larger molecules (polymers). Macromolecules are sorted by their chemical composition into the groups: carbohydrates, lipids, nucleic acids and proteins all of which are essential for living things to. The carbohydrates and lipids are needed for energy. But, carbohydrates are the main source of energy for living things, while, lipids mainly stores the energy. Proteins are used to build and maintain bones including muscle and
LAB REPORT 1st Experiment done in class Introduction: Agarose gel electrophoresis separates molecules by their size, shape, and charge. Biomolecules such as DNA, RNA and proteins, are some examples. Buffered samples such as glycerol and glucose are loaded into a gel. An electrical current is placed across the gel.
0.498 • Plate II o Solution 5: Beverage A Rf: 0.519 o Solution 6: Beverage B Rf: 0.535 o Solution 7: Beverage C Rf: 0.3 Permanganate Test Substance Tested Observations Rxn? Fresh Aqueous Aspartame Stayed purple No Solution 4 Brown w/ precipitate Yes Solution 6 Brown w/ precipitate
At last, the evidence and observations are recorded and are sent on the processing path to analytical testing. To take note, “[f]orensic analysis is concerned not only with the recognition and identification of unknown substances, but also with the individualization and reconstruction of a variety of evidence, events, or conditions” (Zonderman, 1990, p. xiv). The preliminary rounds
We took pictures of each other’s data once finished with the lab. For the paper chromatography, students began by grinding 5g of spinach along with 2g of anhydrous magnesium sulfate. Students added hexanes and acetone as specified by the lab protocols. Once, the solvent was a dark green color, we placed it in a centrifuge and transfer the liquid portion of the solution into a test tube. Throughout this portion of the experiment, students used weighting paper as a funnel poring the indicated solution as stated by the protocol, for instance pouring silica gel and sand into the column. After, we poured about 3ml of Hexanes into the column, making sure not to let the column dry. We then added, spinach extract to the column—after, we added about 1ml of hexanes. Adding hexanes caused the solution to gain a yellow colored band. We added hexanes until the yellow band reached the bottom of the column, thus began to collect all the yellow pigment into a test tube. Once the elutant become colorless, we once again placed a waste basket under it. Finally, we collected the green pigment into another test tube by a 70%/ 30% mixture and a bit of acetone. Once the two colored bands were collected, we obtained the wavelengths of each colored band using the