Egg Albumen Experiment The purpose of this investigation is to establish which is the lowest concentration of Copper (II) Sulphate solution that will denature a sample of egg albumen (egg white) at room temperature. The base of the reaction is the globular protein (albumen) being denatured by a heavy metal (Copper (II)), the copper (II) reacts with the NH3 group causing it to denature, this means the proteins' secondary and tertiary structures are being altered and refolding into different shapes, this resulting in a change from the substance being clear to turning opaque.[1] As the concentration of the denaturants increases more folding and changing of shape will occur and therefore more denaturing will occur and at a faster rate. From this I can predict that that lowest concentration of the solution is approximately at 0.03m solution. The reason this is not lower, is that a lower concentration will have little effect on the protein. To conduct this experiment I will dilute the 0.1mol dm -3 using distilled water. The reason for choosing so is that I have found from previous experiments I have conducted during the year (namely food tests- protein with biuretts reagent) that distilled water is the best diluting agent as its chemical properties will not interfere with the reaction. The procedure for the experiment is as follows: Apparatus list ============== § Test tubes X 11 § 0.10 molar dm -3 Copper (II) Sulphate solution § distilled water § egg albumen from 3 eggs. § Syringe X 12 § colorimeter § tripod § 100ml beaker § Bunsen burner § test tube holder § safety glasses § gloves § test tube pen § test tube method ====== Before conducting the experiment I would conduct a simple test for the protein by placing a sample of the albumen into a test tube and add biurett reagent. This contains copper (II) sulphate and sodium hydroxide.
In the lab the isopods were observed in a way to where behavior and structures could be properly recorded. The isopods were revealed to two dissimilar scenarios, normal temperature water vs. warm temperature water, to calculate which environment was most preferred. In each distinct scenario ten isopods were placed ten a choice chamber, one side being normal temperature (26.7celsius) and the other being warm temperature (43.3 celsius) , and observed for a total of ten minutes with thirty second intervals which was when we recorded our observations. After observations, it was seen that normal conditions was the most preferred environment by the isopods. In the scenario the Isopods exhibited taxis behavior, which is behavior caused by factors such as light, temperature, water and such. Nothing physical, but rather environmental.
The essential points of the green-frosting are the concentration and absorbance value in each diluted which the process of serial dilution. The standard curve of Blue#1 and yellow #5 provide the equation of the trend-line in order to calculate the concentration in the diluted solution of the green frosting. The mole of dye in 100mL green stock solution, mole of dye in 5 gram and 1 gram of frosting, the Beer –Lambert Law, and the compare to amount desired by the company can be determined. The Beer-Lambert Law is the relationship between color and the concentration and equation A=Ebc. The “A” is absorbance, the “C” is a concentration in molarity, the “E” is a molar absorptivity and “b” is the path-length. The goal of the lab is to use the absorbance and the Beer-Lambert law to determine the amounts of blue#1 and yellow #5 in the green frosting.
Introduction: Within this experiment we wish to facilitate a greater understanding of the concepts of experimental design and quantifying techniques. Specifically, this lab will allow us to gain an enhanced understanding of the isolation of a protein using differential solubility, which allows us to separate and purify various proteins using high concentrations of a specific salt so that they may be studied in great detail. Last week we separated our desired protein using ammonium sulfate. Since we have already extracted the desired protein, we will begin quantifying the amount using the Bradford Protein Assay. Because it is a dye-binding assay, we will use the spectrophotometer to measure the absorbance of various dilutions of a protein: this will comprise our standard curve. We will then compare the absorbance of our extracted protein from l...
The article The Egg and the Sperm: How Science has constructed a Romance Based on Stereotypical Male-Female Roles by Emily Martin explains the social constructs of stereotypes and how they are central to our perception of the world around us. Culture is something that shapes how even biological scientists describe what they discover about the natural world. Furthermore, Martin takes a deeper look into the scientific accounts of reproductive technology.
During the incubation, in an Erlenmeyer flask, 1X Tris Acetate EDTA (1 mL) and powder agarose (0.4 g) were dissolved in dH2O (49 mL). Then the solution was microwave for 2 minutes and allowed to cool to room temperature. Then SafeRed concentrate (2.5 µL) was added to the solution and it was poured into the gel box and allowed to solidify.
to see when the x marked paper is not visible. I repeatedly did this 3
tube. Add 6 mL of 0.1M HCl to the first test tube, then 0.1M KMnO4 and