Gel Electrophoresis Lab Report

LAB REPORT
1st Experiment done in class
Introduction:
Agarose gel electrophoresis separates molecules by to their size, shape, and charge. Biomolecules such as DNA, RNA and proteins, are some examples. Buffered samples such as glycerol and glucose are loaded into a gel. An electrical current is placed across the gel. The current moves the molecules towards the cathode or anode. The speed of the moving molecules depends on the size, shape, and charge. The properties of the gel will definitely affect the movement. Small molecules are expected to move easily and faster thru the pores.

Materials and Methods:
Experiment:
1st step to make the gel: pour distilled water and agarose in a beaker.
2nd step heat the mixture: Make sure the agarose dissolves. Wait until it boils and when you are going to transfer the mixture, wear gloves to avoid getting burnt. Transfer the mixture into a removable gel tray.
3rd create wells: put a comb template in middle of the tray; wait until the mixture becomes solid. After,
Discussion:
This experiment demonstrated the ability of agarose gel electrophoresis to separate the mixture of dyes into their individual components by the application of a combination of dyes to the same sample well. The experiment effectively demonstrated that the dyes where different in structure, energy, and composition. Most of the dyes where negatively charged at neutral pHs and only one with positive charge. The positive charge one moved an opposite direction compared to the other dyes.

MLA citation:
#101, Edvo-Kit. Edvo-Kit #101 (n.d.): n. pag. Web.

2nd Experiment done in class