Purpose:
Experiment #1: The purpose of this experiment is to investigate the effects of baking soda and light intensity on the rate of photosynthesis of green spinach leave through the observation of floating disk.
Experiment #2: The purpose of this experiment is to test which colors work best and float while under a different light source.
Experiment #3: The purpose of this experiment to test the chromatography of plant pigments the alcohol test strip test will be used.
Procedure:
Experiment 1: Oxygen Production
I added ½ tablespoon of baking soda to 4 cups of water. I added a small drop of liquid soap to the water and stirred to mix. I used the end of a straw and cut out 20 circles of spinach leaves. I pulled the plunger completely out of the syringe and put the leaf circles into the syringe. Next I pushed the plunger back in. I used the syringe to suck up the baking soda water until the syringe was about ¼ full of liquid. I placed my finger over the end of the syringe and pulled back on the plunger as far as I could without pulling the plunger out. I repeated this step three times. All the leaf circles sunk to the bottom of the liquid. I placed the spinach into a clear glass with about 2 inches of baking soda solution. I blocked out all light. I set the lamp with a compact florescent light bulb. I placed the glass in front of the lamp. I counted the number of circles that floated after each minute for 20 minutes (positive control). I created a negative control by not placing compact florescent light bulb and not placing the glass in front of the lamp. I counted the number of circles that are floating. I repeated the experiment with fresh circles and used regular water plus soap for all steps instead of baking soda and soa...
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... has more CO2 than the negative controls. There will be more spinach circles in the red food coloring than the green and blue food coloring. My hypothesis was supported for both experiments.
To continue the experiment further I can use a different source of light to test the intensity of light. For the food coloring experiment, I could use a different food color such as yellow or orange to test.
Experiment 3:
The red pigment and the green pigment will follow the alcohol higher on the coffee filter than the yellow pigment. There will only be chlorophyll left in the spinach leaf, the yellow leaf will contain chlorophyll and xanthophyll & the red leaf will contain chlorophyll, carotene, and xanthophyll. My hypothesis was supported.
To continue this experiment further, I could use frozen chopped spinach leaves, or substitute the rubbing alcohol for methyl alcohol.
In this experiment I will investigate the affect in which the light intensity will have on a plants photosynthesis process. This will be done by measuring the bubbles of oxygen and having a bulb for the light intensity variable.
Theodor’s experiment was created in part to learn which wavelengths (colors) of light were most effective in carrying out photosynthesis and to prove that it occurs in chloroplast. The experiment was inspired by Theodor’s observation of aerobic bacteria. Theodor observed that aerobic bacteria would move towards the chloroplasts of green algae. Theodor hypothesized that the reason why the bacteria moved toward the chloroplasts was because the organelle generated oxygen via photosynthesis. If photosynthesis occurs in chloroplasts, then the bacteria would aggregate on the chloroplasts producing the most oxygen. Theodor’s experiment was essential because it demonstrated that chloroplasts were the site of photosynthesis. Furthermore,
The lab experiment did prove that Crayola colors are not of pure substances but rather a mixture of specific color compounds needed to produce said colors. The experiment also proved that different color pigments have different densities (the lighter ones moved up the filter paper with the water, while the heavier ones adhered to the filter paper longer.)
The experiment was conducted using carbon dioxide to see how it affected the rate of photosynthesis in spinach leaves. Carbon dioxide should increase the rate of photosynthesis because there will be more carbon dioxide, a reactant in the photosynthesis formula.
The Effect of Light Intensity on the Rate of Oxygen Production in a Plant While Photosynthesis is Taking Place
= > [CH2O} + O2 + H2O, This shows that when the light intensity is increased the rate of reaction will be more quicker he only anomalous result there was, is the one in the 100 watt result the reading after 5 minutes is anomalous because it does not follow the predicted pattern of increasing in the production of gas because it is lower I know from my own knowledge of photosynthesise that when the light intensity is increased the rate of reaction will be more quicker because many plants and trees photosynthesise quicker in stronger light and photosynthesise slower in dimly lit places. The chlorophyll absorbs light energy and enables it to be used by the plant for building up sugar. The overall effect is that energy is transferred from sunlight to sugar molecules.
Planning Firstly here is a list of equipment I used. Boiling tubes Weighing scales Knife Paper towels 100% solution 0% solution (distilled water) measuring beakers potato chips Cork borer. We planned to start our experiment by doing some preliminary work. We planned to set up our experiment in the following way.
The test tubes were labeled. Each of the Erlenmeyer flasks were filed with approximately 225 mL of regular tap water. The two of the aquatic plant Elodea densa was obtained. Each plant was submerged in a solution of 0.5% solution of sodium bicarbonate in a test tube, and approximately 0.5 cm was cut off the stem. A rubber stopper, containing an attached pipette, and syringe and need was placed over each test tube. Then, the stopper was gently pushed into each test tube, making sure no air bubbles were present. Each of the Erlenmeyer flasks containing the test tubes was then placed in front of a lamp. After being calibrated in front of the lamp for 10 minutes, the reading was taken for 40 minutes. Finally the lamp was turned off. Following the experiment, the results were entered into an Excel spreadsheet. Using the online t-test software “graphpad”, an unpaired t-test was generated. In this investigation the light intensity, and temperature, and aquatic plant (Elodea densa) were constant, and thus where the control variables. (Rush,
Materials used in the experiment included 5-7 g of the potato tissue, 50ml of 2.0M phosphate buffer coffee filter and guaiacol dye.
In this experiment, we will look at the concentration of sodium bicarbonate in the light reaction of photosynthesis. This will be carried out using three different colored filters to identify the effect on the light reaction of photosynthesis. Since, photosynthesis is the process whereby light energy is captured by plant, algal, or bacterial cells and is used to synthesize organic molecules from carbon dioxide and water. Although, photosynthesis is atmospheric carbon dioxide is removed and transformed into oxygen and carbohydrates. Elodea is an aquatic plant of a genus that includes the ornamental waterweeds. Sodium bicarbonate is commonly known as baking soda. On the other hand, there are four primary photosynthetic pigments found in the chloroplast
The LED light will make the photosynthesis work even faster so the spinach leaves will probably go up faster than normal. I think that the glucose will help the rate of photosynthesis because it will improve the plant in growth and give the plant more energy. Variables Independent Variable Dependant Variable 3 Control Variable Concentration of Glucose The Rate of Photosynthesis LED light source The distance of the light from the
The question we examined was how are photosynthesis and cellular respiration related? My hypothesis stated that if bromothymol blue, carbon dioxide, water and an Elodea plant are placed into test tube that is exposed to sunlight, then the bromothymol blue would change color which means that the plant has produced oxygen. When Bromothymol Blue is the color blue that indicates that oxygen is present. When Bromothymol Blue is the color green that signifies carbon dioxide is present. On Day 1, the Bromothymol Blue in all the test tubes was a orangish-yellowish-brown color. However, when we examined the Bromothymol Blue on day 2, in the control group, the color stayed same, a orangish-yellowish-brown. In the light test was bluish green and in the
Potassium permanganate test is performed to see the presence of alcohol in this experiment. Five solutions were tested and out of all of them, aqueous methanol and solutions 4 and 6 changed color during the test, meaning that those solutions contained alcohol. Fresh aqueous aspartame and water do not contain alcohol, which is why we saw no color change. Solutions 4 and 6 were hydrolyzed aspartame and diet soda, respectively, under different conditions, and methanol is a product of hydrolysis.
I picked the science experiment elephant toothpaste this year, because it seems interesting and fun. It is also a way to test if the removal of oxygen in these experiments will actually work. I have four different experiments. Two out of the four experiments I am testing, is to see if there is a different reaction time and size between yeast and potassium permanganate and the percentage of the hydrogen peroxide. My third and fourth are tests of elephant toothpaste is to see if adding cornstarch has a color reaction to the potassium iodide to make it look like it is “glowing”, or if the yeast and fluorescent dye will make it look “glowing”. I think the elephant toothpaste that uses 50% hydrogen peroxide and potassium permanganate will have the
An Experiment to Investigate the Effect of Light Intensity on the Rate of Photosynthesis. Introduction Photosynthetics take place in the chloroplasts of green plant cells. It can produce simple sugars using carbon dioxide and water causing the release of sugar and oxygen. The chemical equation of photosynthesis is: [ IMAGE ] 6CO 2 + 6H20 C 6 H12 O 6 + 6O2 It has been proven many times that plants need light to be able to photosynthesize, so you can say that without light the plant would neither photosynthesize nor survive.