When double stranded DNA is heated in a dilute salt solution, its two strands separate because of the breakdown of complementary base pairing (melting). This strand separation is called denaturation. The temperature at which the two complementary strands separate is called the melting temperature ‘Tm’, and is affected by the percentage of G.C base pairs, ion concentration of the solution, presence of destabilising compounds like urea, and the pH of the solution (Lodish et al, 2004, p. 105).
... middle of paper ...
...ected to a probe. These techniques can be used to distinguish between alleles that vary even by single nucleotides (“Nucleic acid hybridization assays”, 1999, Ch. 5).
Nucleic acid hybridisation is used in many routine experiments in the molecular biology laboratory, making it an indispensable requirement in genetic engineering and molecular biology.
“Fluorescent Probes”, n.d. piercenet.com. Retrieved August 2, 2011 from http://www.piercenet.com/browse.cfm?fldID=4DD9D52E-5056-8A76-4E6E-E217FAD0D86B
Lodish, H. et al., 2004. Molecular Cell Biology. W. H. Freeman, New York.
“Nucleic acid hybridization assays”, 1999. Retrieved August 2, 2011 from http://www.ncbi.nlm.nih.gov/books/NBK7567/
Nussbaum, R. L., 2004. Thompson and Thompson genetics in medicine. Elsevier Health Sciences, n.a.
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