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Recombinant dna essay
General paper discussion on genetic engineering
General paper discussion on genetic engineering
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In the 2012 American superhero film The Amazing Spider-Man, the protagonist Peter Parker gets bitten by a genetically altered spider, giving him incredible and enhanced spider-like super powers. The arachnid’s DNA crosses with Parker’s human genome, bestowing him with these abilities. His powers came from a fantasy interpretation of recombinant DNA. In the real world, recombinant DNA can be formed by cloning molecules which allow it to possess the genetic material from multiple sources. This type of cross species genetics is used throughout the world today to artificially select desired traits in “genetically modified organisms” (GMOs). Genetic engineering is at its most advanced state so far right now, letting us intentionally incorporate …show more content…
The birth of genetic engineering and recombinant DNA began in Stanford University, in the year 1970 (Hein). Biochemistry and medicine researchers were pursuing separate research pathways, yet these pathways converged to form what is now known as biotechnology (Hein). The biochemistry department was, at the time, focusing on an animal virus, and found a method of slicing DNA so cleanly that it would reform and go on to infect other cells. (Hein) The medical department focused on bacteria and developed a microscopic molecular messenger, that could not only carry a foreign “blueprint”, or message, but could also get the bacteria to read and copy the information. (Hein) One concept is needed to understand what happened at Stanford: how a bacterial “factory” turns “on” or “off”. (Hein) When a cell is dividing or producing a protein, it uses promoters (“on switches”) to start the process and terminators (“off switches”) to stop the process. (Hein) To form proteins, promoters and terminators are used to tell where the protein begins and where it ends. (Hein) In 1972 Herbert Boyer, a biochemist, provided Stanford with a bacterial enzyme called Eco R1. (Hein) This enzyme is used by bacteria to defend themselves against bacteriophages, or bacterial viruses. (Hein) The biochemistry department used this enzyme as a “molecular scalpel”, to cut a monkey virus called SV40. (Hein) What the Stanford researchers observed was that, when they did this, the virus reformed at the cleaved site in a circular manner. It later went on to infect other cells as if nothing had happened. (Hein) This proved that EcoR1 could cut the bonding sites on two different DNA strands, which could be combined using the “sticky ends” at the sites. (Hein). The contribution towards genetic engineering from the biochemistry department was the observations of EcoR1’s cleavage of
When a new GMO (Genetically Modified Organism) is released into commercial markets, severe testing is required. Years of feeding tests on animals with similar diets to humans must be performed, along with a chemical analysis of the product and various tests on the host plant or animal itself to see if the mutation is stable (“Evaluating Safety: A major undertaking”). However, humans can’t just be dissected and dissolved to find out if a genetic treatment works. Animal testing has been suggested, but not only is that amoral, it isn’t reliable. While similarities exist between animal and human working DNA (DNA that expresses a physical trait) (Marder), there is still a difference of roughly fifteen-million base pairs between a human and the closest animal counter part (“What Are Our Closest Animal Relatives?”). After including non-working DNA (dormant or “junk” DNA that may code for a trait but is not in use), which accounts for 80% of our entire genome (Marder), differences begin to accumulate, making animals an unreliable test subject, leaving human beings as the only viable test
Viruses are the smallest, simplest living things, smaller than bacteria, and the cause of some of the deadliest diseases known to humanity. They are composed chiefly of nucleic acid wrapped in a coat of protein and are able to multiply only from within living cells. As with all other organisms, the virus depends for its ability to obtain energy and carry out the other processes necessary to sustain life, upon its stock of DNA, the hereditary material that makes up the genes, the "instructions" that determine the traits of every living organism. What is interesting about viruses, however, is that their genetic stock is very meagre indeed, so much so that reliance upon it alone cannot enable them to survive. Nonetheless, viruses do persist from one generation to the next, as if they were alive. How this is managed, as it clearly is in both plants, animals and human beings, bears importantly upon the ways in which "life", at least in the case of viruses, may legitimately b...
Genetic engineering has been around for many years and is widely used all over the planet. Many people don’t realize that genetic engineering is part of their daily lives and diet. Today, almost 70 percent of processed foods from a grocery store were genetically engineered. Genetic engineering can be in plants, foods, animals, and even humans. Although debates about genetic engineering still exist, many people have accepted due to the health benefits of gene therapy. The lack of knowledge has always tricked people because they only focused on the negative perspective of genetic engineering and not the positive perspective. In this paper, I will be talking about how Genetic engineering is connected to Brave New World, how the history of genetic engineering impacts the world, how genetic engineering works, how people opinions are influenced, how the side effects can be devastating, how the genetic engineering can be beneficial for the society and also how the ethical issues affect people’s perspective.
A GMO is a plant or animal that has been genetically engineered with DNA from bacteria, viruses, or other plants and animals. Most of the combinations which are used could not possibly occur in nature on its own. The intention of the process is to create a new beneficial trait such as creating its own pesticide or make it immune to herbicides. This would allow the crop such as Bt co...
DARPA (Defense Advanced Research Projects Agency) is currently working on a project on upgrading soldiers’ physical abilities, the soldiers of the future will be genetically modified into super soldiers capable of some usual super feats. Such abilities includes making the soldiers able to run at Olympic without special training, carry huge weights, be able to go for days without food or sleep, immunity to pain, faster healing and ability to regrow broken or lost limbs. This technology is a study involves genetic engineering. Genetic engineering means the change and modification of the characteristics of an organism by controlling its genetic material. The genetic material can be a part or group of genes, a whole or group of DNA molecules, a fragment of DNA or the entire genome. (BBC) The genes contain the information of what we look like, our h...
Genes are, basically, the blueprints of our body which are passed down from generation to generation. Through the exploration of these inherited materials, scientists have ventured into the recent, and rather controversial, field of genetic engineering. It is described as the "artificial modification of the genetic code of a living organism", and involves the "manipulation and alteration of inborn characteristics" by humans (Lanza). Like many other issues, genetic engineering has sparked a heated debate. Some people believe that it has the potential to become the new "miracle tool" of medicine. To others, this new technology borders on the realm of immorality, and is an omen of the danger to come, and are firmly convinced that this human intervention into nature is unethical, and will bring about the destruction of mankind (Lanza).
Watson, J. D., Gilman, M., Witkowski, J., Zoller, M. (1992). Recombinant DNA. New York: W. H. Freeman and Company.
Science and technology are rapidly advancing everyday; in some ways for the better, and in some, for worse. One extremely controversial advance is genetic engineering. As this technology has high potential to do great things, I believe the power genetic engineering is growing out of control. Although society wants to see this concept used to fight disease and illness, enhance people 's lives, and make agriculture more sustainable, there needs to be a point where a line is drawn.
In this day and age, Genetically Modified Organisms (GMOs) have become a topic of large interest in the media. GMOs are defined as an organism whose genetic structure has been altered by incorporating a gene that will express a desirable trait (Dresbach et al. al. 2013). Often times, these traits that are selected are either beneficial to the consumer or producer. Currently, GMOs are being created at a higher rate than ever before and are being used in the foods that we eat.
Human genetic engineering can provide humanity with the capability to construct “designer babies” as well as cure multiple hereditary diseases. This can be accomplished by changing a human’s genotype to produce a desired phenotype. The outcome could cure both birth defects and hereditary diseases such as cancer and AIDS. Human genetic engineering can also allow mankind to permanently remove a mutated gene through embryo screening as well as allow parents to choose the desired traits for their children. Negative outcomes of this technology may include the transmission of harmful diseases and the production of genetic mutations. The benefits of human genetic engineering outweigh the risks by providing mankind with cures to multiple deadly diseases.
The Double Helix tells a tale of fierce competition, perseverance, and scientific innovation as we follow James Watson and his cohort Francis Crick on their quest to discover the secret to life, the structure of deoxyribonucleic acid. Although already fascinated with DNA, Watson struggled with finding chemistry exciting enough to learn it in depth. He had studied birds in college and thereby managed to avoid any formal chemistry or physics courses. As he later pursued a PhD in biochemistry, he realized he could put it off no longer and attempted to learn organic chemistry at Indiana University. However, after a mishap in the lab, he was encouraged instead to study nucleic acid chemistry with Herman Kalckar in Copenhagen. There, his mind strayed from his work and he began doing unauthorized research in the lab of Ole Maaløe, studying phages. Herman stopped teaching Watson after going through a divorce with his wife, and sent Watson off to a scientific conference in Naples. Although he was bored by many of the lectures, Maurice Wilkins’s talk about X-ray diffraction fascinated Watson. He was struck by an X-ray diffraction picture of DNA that Maurice presented and was determined to study the acid. He later got to know more about Maurice’s colleague, Rosalind Franklin, who was proud, stubborn, and very difficult to work with. Watson greatly admired the lecture given by the renowned Linus Pauling, who had discovered the structure of the alpha-helix and was thought of as the leader in DNA research in the scientific world.
The Use of Recombinant DNA Technology Recombinant DNA technology is the technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism.(1) This is the process of using recombinant DNA technology to enable the rapid production of human protein from a single gene of insulin. Firstly the single gene required must be isolated. This can be done three ways: Either by working backwards from the protein- Finding the amino acid sequence for the protein needed, the order of bases can be established using known genetic code. New DNA can be made from this sequence of bases resulting in artificial gene made from complementary DNA.
Discoveries in DNA, cell biology, evolution, and biotechnology have been among the major achievements in biology over the past 200 years, with accelerated discoveries and insight’s over the last 50 years. Consider the progress we have made in these areas of human knowledge. Present at least three of the discoveries you find to be the most important and describe their significance to society, health, and the culture of modern life. DNA (deoxyribonucleic acid) is a self-replicating molecule or material present in nearly all living organisms as the main constituent in chromosomes. It encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses.
Since school days the terms like cell, genes, DNA, protein intrigued me a lot and helped me in realizing the dream of pursuing a career in Biotechnology field that has been making many remarkable achievements. The passion towards biological sciences made me to take B.TECH in Biotechnology in Dr. M.G.R. University.
In the early years of biotechnology,amateur biotechnologists, had no idea their ideas would snowball into incredible modern discoveries. Early discoveries included the processes of fermentation, pasteurization, and zymotechnology. The products of these processes are wine, cheese, and beer; all of which contributed significantly to the gross national product of their respective countries. However, it wasn’t until the discovery of the structure of DNA by Watson and Crick in 1953, that genetics and biotechnology would be linked together to explain so many of these discoveries.