Chain Reaction

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  • Polymerase Chain Reaction

    999 Words  | 4 Pages

    cloning is viewed at a genetic molecular level, where a piece of DNA is copied on a large-scale by genetically copying tens to hundreds of thousands of identical DNA fragments. Researchers are developing new methods of cloning by using polymerase chain reaction (PCR). PCR was introduced in the 1980s and in recent years Kary Mullis won the Nobel Prize in Chemistry for his invention of PCR. Today, Scientists today are researching the various sub-fields of cloning, using PCR, in new ways using terminators

  • Polymerase Chain Reaction

    912 Words  | 4 Pages

    PCR (Polymerase Chain Reaction) is the quick and easy method of making unlimited copies of any fragment of DNA. Since it’s first introduction ten years ago, PCR has very quickly become an essential tool for “improving human health and human life (TPCR)”. Medical research and clinical medicine are profiting from PCR mainly in two areas: detection of infectious disease organisms, and detection of variations and mutations in genes, especially human genes. Because PCR can amplify unimaginably tiny amounts

  • Polymerase Chain Reaction

    538 Words  | 3 Pages

    The first PCR reaction was accomplished with only reverse primers, with this program: Reverse primer (50 pmol), 20 ng/l template, and 69°C annealing temperature in 12 cycles, followed by another PCR reaction with forward and reverse primers for 29 cycles, and equal amount of primers (10 pmol). The PCR product was extracted from agarose gel after gel electrophoresis using gel extraction kit (Qiagen Company) and ligated in gWiz plasmid (Aldevron) using T4 ligase (Intron, Korea) between SalI and EcoRV

  • Polymerase Chain Reaction Lab

    728 Words  | 3 Pages

    Title: Polymerase Chain Reaction Simulation Propose: The propose of this lab was to understand how by running a gel electrophoresis on a batch of DNA we are able to see how many approximately cycles it has gone through. Methods: Casting the Agarose Gel In this experiment .8% solution was used. By using a 250ml flask the buffer solution was prepared. Using the equation to make enough solution for the entire lab class the equation had to be multiplied by four. The contents of this equation were added

  • Hamlet - Revenge A Chain Reaction

    872 Words  | 4 Pages

    Hamlet Revenge: A Chain Reaction     In the play Hamlet, by William Shakespeare, the theme of revenge is repeated numerous times throughout the play and involves a great deal of characters. Of these characters, eight are dead by the end of the play by result of murder which was initiated through revenge. Shakespeare uses the revenge theme to create conflict among many characters.     Shakespeare uses the revenge theme to create conflict between Hamlet and Claudius

  • What is the Polymerase Chain Reaction (PCR)

    1027 Words  | 5 Pages

    The polymerase chain reaction or PCR for short can be used to create many copies of DNA. This allows the DNA to then be visualized using a dye like ethidium bromide after gel electrophoresis. The process has been refined over the years, however the basic steps are similar. The first is to denature dsDNA through heating to ~96 °C. This separates the two strands of DNA. The exact temperature to be used can be calculated with Tm = 4oC x (no. of G & C) + 2oC x (no. of A & T). Tm is the melting point

  • Malala Yousafzai 's Chain Reaction of Influence

    630 Words  | 3 Pages

    Malala Yousafzai Malala Yousafzai stood up for herself, her education, and for her fundamental rights when confronted by a fearsome terrorists group. Malala created a chain reaction around the world bringing change, light, and hope to girls across the world. Malala was born July 12, 1997, in Mingora, Pakistan, Her father is Ziauddin Yousafzai. She became an advocate for girls’ education. When she was 14 years old, she got shot in the head by a Taliban. Malala and her family found out that a Taliban

  • EVALUATION OF MULTIPLEX POLYMERASE CHAIN REACTION USING TWO PRIMERS AND ITS COMPARISON WITH CONVENTIONAL ISOLATION TECHNIQUES FOR THE DIAGNOSIS OF...

    529 Words  | 3 Pages

    Patients and samples This study was approved by the Institutional Ethics Committee. After taking written informed consent of the subjects, total of 115 CSF samples were collected from patients more than 12 years of age irrespective of HIV status admitted to Nehru Hospital, Post Graduate Institute of Medical Education and Research (PGIMER). All the samples were subjected to Ziehl Neelsen (ZN) staining, conventional Lowenstein Jensen (LJ) culture, BACTEC Mycobacterial growth indicator tube (MGIT) liquid

  • EVALUATION OF MULTIPLEX POLYMERASE CHAIN REACTION USING TWO PRIMERS AND ITS COMPARISON WITH CONVENTIONAL ISOLATION TECHNIQUES FOR THE DIAGNOSIS OF...

    605 Words  | 3 Pages

    A total of 55 subjects were grouped into ‘confirmed’ TBM (n=9)’ and ‘suspected’ TBM (n=46) categories. The 60 control subjects included patients who presented with bacterial, viral, fungal or non infectious cause of CNS symptoms. The results of AFB smear, LJ and BACTEC culture and different PCR methods between cases and controls were compared (Table 3). Figure 1 shows the gel photograph of multiplex PCR (multiplex PCR is considered as positive when the band is present for MPB64 or IS6110 or both)

  • EVALUATION OF MULTIPLEX POLYMERASE CHAIN REACTION USING TWO PRIMERS AND ITS COMPARISON WITH CONVENTIONAL ISOLATION TECHNIQUES FOR THE DIAGNOSIS OF...

    868 Words  | 4 Pages

    multiplex PCR as compared to smear and culture. Moreover, using multiplex PCR appears to be a necessity for rapid diagnosis of TBM cases in a developing country like India. Our study suggests use of more than one targets (MPB64 and IS6110) together in a reaction thereby reducing the contamination rate and the possibility of false negative results in CSF samples of tuberculous meningitis patients particularly in countries with high burden of the disease and circulating M. tuberculosis strains lacking IS6110

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