The Resistance Of Bacteria And The Cell Number / High Density Populations

The Resistance Of Bacteria And The Cell Number / High Density Populations

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Bacteria are social microorganisms that demonstrate group behaviors such as the ability to communicate by producing, releasing, detecting and responding to small signaling molecules. This process is termed quorum sensing (QS). It allows a population of bacteria to monitor the environment for the presence of other bacteria and to modulate the population’s behavior in response to the quantity of microbial species present in a neighboring community. Although there has been significant effort toward understanding QS, few studies have explored this complex biological process in low-cell-number/high-density populations. Such studies are of great medical significance since numerous infectious diseases are initiated by small bacterial populations arranged into dense clusters. The Shear lab has developed a µ3D-printing technology to pattern responsive micro-enclosures allowing us to study bacteria in controlled 3D environments. Here, we propose the use of this method in combination with two analytical sensing techniques: a custom-built optrode-based µ-biosensor and transparent carbon ultramicroelectrode arrays (T-CUAs). By using these approaches, we will be able to observe bacterial responses to different chemical environments in real time.

(A) A list of references for the context for the work I am doing:

(1) Kaehr, B.; Shear, J. B. Mask-Directed Multiphoton Lithography. J. Am. Chem. Soc. 2007, 129 (7), 1904–1905.

Summary: This paper demonstrates the mask-directed multiphoton lithography method developed by the Shear lab. In this approach, electronic mask objects are used to promote protein cross-linking to create biomaterials where single motile bacterium can be incubated.

(2) Nielson, R.; Kaehr, B.; Shear, J. B. Microreplication an...

... middle of paper ...

...based system developed to specifically identify E. coli labeled with fluorescent dyes. This reference will be used as an example of bacterial optrode-based sensors that has been developed.

(B) What has our group done so far to bring me to the work I am doing now?
• µ3D-printing developed by the Shear lab to create very dense bacterial communities and the electrochemical platform developed by the Stevenson lab – T-CUAs for sensing cellular communication. Pros/cons of each technique.
• µ-biosensor being designed by the Shear lab – sensing through an optical fiber. Construct an in vitro platform to transition to in vivo sensing.
• These analytical techniques will allow for studying very complex biological systems. It is important that we understand these systems --- host/pathogen interactions in chronic wounds.

(C) Summary of what I will talk about in the proposal.

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