(A) A list of references for the context for the work I am doing:
(1) Kaehr, B.; Shear, J. B. Mask-Directed Multiphoton Lithography. J. Am. Chem. Soc. 2007, 129 (7), 1904–1905.
Summary: This paper demonstrates the mask-directed multiphoton lithography method developed by the Shear lab. In this approach, electronic mask objects are used to promote protein cross-linking to create biomaterials where single motile bacterium can be incubated.
(2) Nielson, R.; Kaehr, B.; Shear, J. B. Microreplication an...
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...based system developed to specifically identify E. coli labeled with fluorescent dyes. This reference will be used as an example of bacterial optrode-based sensors that has been developed.
(B) What has our group done so far to bring me to the work I am doing now?
• µ3D-printing developed by the Shear lab to create very dense bacterial communities and the electrochemical platform developed by the Stevenson lab – T-CUAs for sensing cellular communication. Pros/cons of each technique.
• µ-biosensor being designed by the Shear lab – sensing through an optical fiber. Construct an in vitro platform to transition to in vivo sensing.
• These analytical techniques will allow for studying very complex biological systems. It is important that we understand these systems --- host/pathogen interactions in chronic wounds.
(C) Summary of what I will talk about in the proposal.
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