PCR is a technique to amplify as little as a single or as many as ten copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. This method relies on thermal cycling, consisting of enzyme repeated heating and cooling cycles aimed at replication of DNA. Primers containing sequences complementary to the target region along with the DNA polymerase are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is used as a template for replication. As a result the polymerase chain reaction can be extensively modified to perform a wide array of genetic manipulations.
In Chemistry, medicine and genetic analysis 2006, (RSC, Advancing the Chemical Sciences) S...
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...ines for cloning PCR.
Cloning, as we know is a very complex, complicating topic. But once we know the fundamentals of science, various ideas come out and research begins. Researchers today are making PCR more effective in the amount of genetic material produced, becoming cheaper, and testing new methods of cloning with the application of PCR.
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Brown, Tom. "Chemistry, medicine and genetic analysis." RSC Advancing the Chemcial Sciences (2006).
Costa, G. L., A. Grafasky, and M. P. Weiner. "Cloning and analysis of PCR-generated DNA fragments." Genome Research 3 (1994): 338-45.
"Polymerase chain reaction -." Wikipedia, the free encyclopedia. Web. 19 Jan. 2010.
Zhou, Ming-Yi, and Celso E. Gomez-Sanchez. "Unversal TA Cloning." Molecular Biology 1st ser. 2 (2000): 1-7.
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